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Inhibition of raffinose family oligosaccharides and galactosyl pinitols breakdown delays germination of winter vetch [Vicia villosa Roth.] seeds

100%

Lahuta L. B., Goszczynska J.

Acta Societatis Botanicorum Poloniae

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2009

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tom 78

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nr 3

203-208

Beside RFOs, which are commonly present in legume seeds, seeds of some species contain galactosyl pinitols (GPs). These carbohydrates, like RFOs, have been hypothesized to constitute an important energy and carbon skeletal source during germination. To test this hypothesis we have applied a specific α-galactosidase inhibitor (1-deoxygalactonojirimycin, DGJ) to germinating winter vetch (Vicia villosa Roth.) seeds, containing more galactosyl pinitols than RFOs. The breakdown of RFOs but not that of GPs was completely blocked in both embryonic axes and cotyledons tissues, during the first 18 h of imbibition in DGJ. The inhibitor only decreased the rate of GPs degradation. The inhibitory effect of DGJ on GPs degradation was partially alleviated by addition of sucrose or galactose to DGJ solutions. After three days of germination in water, RFOs and GPs disappeared in axial tissues of seeds imbibed in water, galactose or sucrose. Eighteen-hour imbibition of seeds in DGJ drastically reduced germination, by ca 50%, during the first three days. The inhibitory effect of DGJ decreased during the next seven days of germination. The presence of galactose or sucrose in imbibition solution initially stimulated seed germination, but later this effect was not statistically significant. Our study provides clear evidence that galactosyl pinitols play an important role in early winter vetch seeds germination. Additionally, we suggest that galactosyl pinitols can replace RFOs as reserve material necessary for early germination.

2

Influence of synbiotics delivered in ovo on immune organs development and structure

100%

Slawinska A., Siwek M., Zylinska J., Bardowski J., Brzezinska J., Gulewicz K. A., Nowak M., Urbanowski M., Plowiec A., Bednarczyk M.

Folia Biologica

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2014

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tom 62

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nr 3

3

The effect of galactinol synthase activity on RFOs content in developing pea seeds (Pisum sativum L.) subjected to drying and cold

100%

Gojlo E., Szymczak M., Kondrat B.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2013

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tom 94

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nr 3

4

Differences in accumulation of soluble alpha-galactosides during seed maturation of several Vicia species

100%

Lahuta L. B., Gorecki R. J., Gojlo E., Horbowicz M.

Acta Physiologiae Plantarum

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2005

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tom 27

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nr 2

Composition and levels of soluble α-galactosides: raffinose family oligosaccharides (RFOs) and galactosyl cyclitols (Gal-C) in developing seeds were measured by high resolution gas chromatography (HRGC) method. The studies were performed on maturing seeds of several wild and cultivated Vicia species: Viciaangustifolia L.(commonvetch), Vicia craccaL. (bird vetch), Vicia grandiflora Scop. (large yellow vetch), Vicia hirsuta (L.) S.F.Gray (tiny vetch), Vicia sativa L. (garden vetch, spring-growing cultivar Kwarta), and Vicia villosa Roth (winter vetch). In all Vicia species similar patterns in the accumulation of RF Os were observed. Galactinol - the donor of galactosyl moieties in α-galactosides biosynthesis was present in the middle stage of seed development, before appearing measurable levels of RFOs. Accumulation of RFOs started parallel with seed desiccation process. At first accumulation of the raffinose, then few days later stachyose and finally verbascose was noticed. In the final stage of seed maturation the verbascose was the main soluble α-galactoside (up to 3 % of dry weight, V. sativa). Besides the RFOs seeds of three Vicia species (V. cracca, V. hirsuta, and V. villosa) accumulated D-pinitol and its α-galactosides (Gal-C). Mono-galactosylpinitols (similar to raffinose) appeared in these species 2-4 days after galactinol, di-galactosyl pinitol A (common name: ciceritol) and di-galactosyl myo-inositol were present several days later than raffinose, and accumulation of tri-galactosyl pinitol A (TGPA) began after accumulation of stachyose. Matured seeds of V. hirsuta contained much more RFOs than Gal-C, opposite to seeds of V. villosa, and V. cracca where concentration of Gal-C was 4-8-fold higher than RFOs. In V. cracca seeds RFOs were almost replaced by Gal-C. In seeds of V. cracca and V. villosa the level of D-pinitol was significantly higher, than the level of myo-inositol. Contents of both cyclitols declined rapidly at the beginning of seed desiccation, when accumulation of RFOs and Gal-C quickly ini creased. We suggest that α-galactosides of D-pinitol can substitute raffinose family oligosaccharides and play similar role during seed maturation and storage.

5

Different effects of soil drought on soluble carbohydrates of developing Lupinus pilosus and Lupinus luteus embryos

80%

Piotrowicz-Cieslak A. I., Michalczyk D. J., Adomas B., Gorecki R. J.

Acta Societatis Botanicorum Poloniae

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2007

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tom 76

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nr 2

The aim of this study was to compare the accumulation of soluble carbohydrates in embryos of two lupin species: cultivated Lupinus luteus (cv. Juno) and wild L. pilosus, developing on plants grown under normal soil humidity and soil drought. All analysed seeds accumulated soluble carbohydrates, including: monosaccharides, sucrose, cyclitols, galactosyl cyclitols and raffinose family oligosaccharides. Soil drought caused a nearly two-fold increase of soluble carbohydrate contents in both species. L. pilosus embryos however, responded to water deficiency by increasing the accumulation of cyclitols and galactosyl cyclitols, whereas L. luteus embryos enhanced accumulation of cyclitols and raffinose family oligosaccharides.

6

Products and biopreparations from alkaloid-rich lupin in animal nutrition and ecological agriculture

80%

Ciesiolka D., Gulewicz P., Martinez-Villauenga C., Pilarski R., Bednarczyk M., Gulewicz K.

Folia Biologica

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2005

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tom 53

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nr Suppl.

7

Composition of seed soluble carbohydrates and ultrastructural diversity of testa in lupins from the Mediterranean Region

80%

Piotrowicz-Cieslak A. I.

Acta Societatis Botanicorum Poloniae

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2005

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tom 74

|

nr 4

This article present a comparison of soluble sugar levels in seeds of Lupinus atlanticus, Lupinus cosentinii, Lupinus palaestinus and Lupinus pilosus, Lupinus hispanicus subsp. hispanicus and Lupinus luteus of Juno variety. Considering that sugars are accumulated in embryos, only the embryonic tissues were used for biochemical analyses. Additionally, the share of testa and embryo in seed tissues was evaluated. The seed-coat thickness was measured using scanning electron microscopy. The seed coat had the largest share in seeds of Lupinus pilosus and Lupinus palaestinus, and the least share in seeds of Lupinus hispanicus subsp. hispanicus and Lupinus luteus of Juno variety. In the seed of Lupinus pilosus the thickness of the seed coat was 1100 µm, while in Lupinus luteus it was 300 µm. The analysed Lupinus seeds accumulated from 73 mg/g d.m. (dry matter of seed embryo) to 155 mg of soluble sugars/g d.m. The highest quantity of oligosaccharides of the raffinose series was found in seeds of Lupinus luteus, while the lowest amount in seed of Lupinus palaestinus. Galactosyl cyclitols appeared in largest amount in seeds of Lupinus palaestinus and Lupinus pilosus, appropriately 41.93 and 33.75 mg/g dm. The lowest amount of galactosyl cyclitols appeared in Lupinus atlanticus, Lupinus cosentinii and Lupinus hispanicus.

Ograniczanie wyników

Inhibition of raffinose family oligosaccharides and galactosyl pinitols breakdown delays germination of winter vetch [Vicia villosa Roth.] seeds

Influence of synbiotics delivered in ovo on immune organs development and structure

The effect of galactinol synthase activity on RFOs content in developing pea seeds (Pisum sativum L.) subjected to drying and cold

Differences in accumulation of soluble alpha-galactosides during seed maturation of several Vicia species

Different effects of soil drought on soluble carbohydrates of developing Lupinus pilosus and Lupinus luteus embryos

Products and biopreparations from alkaloid-rich lupin in animal nutrition and ecological agriculture

Composition of seed soluble carbohydrates and ultrastructural diversity of testa in lupins from the Mediterranean Region