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The response of radish seedlings (Raphanus sativus L. subvar. radicula Pers.), as non-target plant, to various doses of glyphosate applied to root zone was studied in the experiment. The glyphosate was used at concentrations 0.1, 0.5 and 2.0 mM, and the study was conducted on seedlings grown in hydroponic cultures in controlled light and temperature conditions. In the experiment, roots of seedlings were exposed to glyphosate for 4, 7 or 14 days. In order to evaluate the effect of glyphosate, length and biomass of the seedling organs, as well as contents of anthocyanins in hypocotyls and cotyledons have been measured. Glyphosate applied to root zone had considerably higher impact on the growth of the primary root than shoot of radish seedlings. Short-term exposure to glyphosate led to the stimulation of growth and biomass organs of the radish seedling, but such treatment had no effect on the contents of anthocyanins in the cotyledons and hypocotyl. However, after longer exposure to glyphosate a decrease of anthocyanins content in the hypocotyl and its increase in the cotyledons was noted.
Forty safflower genotypes were grown under normal irrigation and drought stress. In the first experiment, the allelopathic potential of shoot residues was evaluated using the sandwich method. Each genotype residue (0.4 g) was placed in a sterile Petri dish and two layers of agar were poured on that. Radish seeds were placed on agar medium. The radish seeds were cultivated without safflower residues as the controls. The length of the radicle, hypocotyl, and fresh biomass weight and seed germination percentages were measured. A pot experiment was also done on two genotypes with the highest and two with the lowest allelopathic activity selected after screening genotypes in the first experiment. Before entering the reproductive phase, irrigation treatments (normal irrigation and drought stress) were applied. Shoots were harvested, dried, milled and mixed with the topsoil of new pots and then radish seeds were sown. The pots with safflower genotypes were used to evaluate the effect of root residue allelopathy. The shoot length, fresh biomass weight, and germination percentage were measured. Different safflower genotypes showed varied allelopathic potential. The results of the first experiment showed that Egypt and Iran-Khorasan genotypes caused maximum inhibitory responses and Australia and Iran-Kerman genotypes resulted in minimum inhibitory responses on radish seedling growth. Fresh biomass weight had the most sensitivity to safflower residues. The results of the pot experiment were consistent with the results of in vitro experiments. Residues produced under drought stress had more inhibitory effects on the measured traits. Safflower root residue may have a higher level of allelochemicals or different allelochemicals than shoot residue.
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The use of high quality sowing material is the fundamental condition for good yielding of crop plants. One of new, unconventional methods of seed quality improvement is low-frequency variable magnetic field. It has been found that this physical factor has a favourable effect on seed germination, emergence and growth of many crop plants. The objective of the study was to determine the effect of low-frequency magnetic field (LFMF) on the germination of radish seeds, with particular emphasis on old seeds. The experimental material were seeds of 7 lots of radish cv. ‘Mila’, with germination capacity of 66.5–92.5%. The age of the seeds was from 1 to 8 years. Seeds from all the lots were treated with variable magnetic field with frequency of 50 Hz, at 3 doses of magnetic induction as follows: 0 (control), 30 and 60 mT for 30 seconds. Then, the seed germination energy and capacity were determined, as well as the length of the hypocotyl and of the radicle of the seedling, dry weight of seedling, and emergence and mean time of emergence. The low frequency magnetic field exposure (30 and 60 mT) improved radish seed germination energy and capacity of every old seed lot. The increase of germination energy of old seeds amounted to 12.3–19.2%, and the increase of germination capacity was 5.8–10%. Magnetic field stimulation caused the increase of germination energy for 3 out of 4 seed lots of high quality but did not affect the improvement of germination capacity of those lots. No significant effect of magnetic field of seeds on the hypocotyl and radicle elongation was observed with the exception of 1 out of the 7 seed lots. For the 2 old seed lots out of the 3 total, magnetic field treatment caused the increase of emergence by 4.4–13.0%.
NADP-isocitrate dehydrogenase (NADP-ICDH) activity of radish (Raphanus sativus L.) seedlings pretreated with various plant growth regulators: KiN, GA, and ABA and different nitrogen sources viz. KNO₃, NH₄Cl and NH₄NO₃ in light and dark was investigated. ICDH activity was significantly higher in light than in dark; addition of different nitrogen sources reduced it to a greater extent in NO₃ supplementation. Among hormonal treatment only KiN showed slight promotion with KNO₃ and NH₄NO₃. On the other hand in light KNO₃ and/or NH₄NO₃ promoted ICDH activity and among hormones, KiN significantly promoted the activity in KNO₃ and NH₄NO₃ supplemented seedlings while ABA was effective in NH₄CL. It is suggested that in non-photosynthetic tissues, NADP-ICDH provides both reductant and carbon skeleton for glutamate synthesis.
Field experiments were carried out over 2002-2004 at the Experiment Station of the University of Technology and Agriculture at Mochełek, in the vicinity of Bydgoszcz, on a very good rye complex soil. High biomass yields were recorded for all the nonpapilionaceous plants researched, grown in stubble intercrop. Upon no nitrogen fertilization, sunflower showed to be most productive. The treatments which involved a high nitrogen dose (90 kg·ha-1) oil radish yielded significantly higher that phacelia. The plant produced also significantly more post-harvest residue mass than sunflower and phacelia, and also used nitrogen applied prior-to-sowing most considerably. An increase in the dose from 0 to 90 kg of nitrogen resulted in an increase in its accumulation in the plant biomass by 74.4 kg (82.7% of the nitrogen applied), as compared with the non-fertilized treatments. Sunflower used only 49.0 kg (54.4%), while phacelia – 48.2 kg (53.6%). The nitrogen fertilization applied in the experiments significantly increased the overground plant biomass yield of the crops researched. The greatest reaction to N fertilization was demonstrated by oil radish. The dry matter yield of this plant increased by 26.5% due to the application of 45 kg·ha-1, as compared with the yield collected from unfertilized objects. Increasing the nitrogen dose from 45 to 90 kg·ha-1 resulted in an 18.4% increase in the dry matter yield of radish. The effectiveness of nitrogen fertilization of sunflower was definitely lower and was, respectively, 14.6 and 7.0%. There was observed a relatively low infection with fungal pathogens in sunflower and phacelia, much higher in radish. A significant effect of the fertilization dose on the health status of the plant roots was found. Sunflower roots were most healthy when 45 kg·ha-1 was applied, and the most heavily infected when treated with 90 kg N·ha-1. Radish fertilization with nitrogen, irrespective of the dose, increased the root infection with fungi. There was found no clear effect of the nitrogen fertilization dose on the health status of phacelia roots. The pathogenic fungi isolated from infected roots of plants grown as intercrops were dominated by Alternaria alternata, as well as Fusarium spp., especially F. solani, F. avenaceum and F. culmorum.
Intergeneric hybrids between non-heading Chinese cabbage (Brassica campestris ssp. chinensis Makino; 2n = 4x = 40) and radish (Raphanus sativus L.; 2n = 4x = 36) were obtained through ovary culture and embryo rescue. Some hybrid embryos (0.11 per ovary) were produced, but only 4 of them germinated. As most hybrid embryos failed to develop into plantlets directly, plants were regenerated by inducing shoots on the cultured cotyledon and inducing roots on the root induction medium. All hybrid plants were morphologically uniform. They resembled the non-heading Chinese cabbage in the long-lived habit, the plant status, the vernalization requirement and the petiole color, while the petiole shape, leaf venation pattern and flowers were more similar to those of radish. Upon examination of the flowers, these were found to have normal pistil, but rudimentary anthers with non-functional pollen grains. The somatic chromosome number of F1 plants was 38. Analysis of SSR banding patterns provided additional confirmation of hybridity.
Changes in the thiocyanate content in hypocotyl-roots and leaves of radish were observed in a two-year field experiment. Six cultivars were tested: early radish (Rex and Ostergruss Różowa), Japanese radish (Tokinashi and Minowase Summer Cross F₁), and winter radish (Monachijska Biała and Murzynka). A significant diversification in thiocyanate content among cultivars, plant parts, harvest dates and observation years was found. Early cultivars contained the least amount of these compounds, Murzynka — the greatest. The content of thiocyanates in leaves was 3 – 5 times higher than that in hypocotyl-roots. The changes in the thiocyanate content during root growth showed a constant rising tendency in the case of the leaves of all cultivars and the storage organs of Murzynka.
The objective of this study was to evaluate the effect of different CO2 concentrations on carbohydrate, chlorophyll contents and net photosynthetic productivity in radish (Raphanus sativus L., cv. Zara) leaves. Plants were exposed to 350, 700, 1,500 and 3,000 ppm atmospheric CO2 concentrations over ten days. Day/ night temperature was 24°C/17°C and photoperiod was 16 h. Carbohydrate (fructose, glucose, sucrose and maltose) content analysis was performed using chromatographic methods. The content of chlorophyll was evaluated spectrophotometrically. The results showed that elevated CO2 increases total carbohydrate con­tent and changes in hexoses/sucrose ratio. A significant increase in chlorophyll content was only in 1,500 ppm treatment. Differences in photosynthetic productivity rate were within error margins. There was no ef­fect on carbohydrate and chlorophyll contents in radish leaves seven days after returning plants to ambient CO2, though higher photosynthetic productivity rate was in radish, previously grown under 700 ppm CO2. In summary, leaf carbohydrate contents affect the intensity of photosynthetic pigment synthesis.
An approach which could be used for quick searches for RAPD markers is described for groups of radish lines with certain morphological traits. The lines are characterized by various morpho-physiological abnormalities, including tumor formation (lines 12, 19, and 21) and non-terminal development of the flower meristem as a variant of tumor growth (line 6). We found four markers which differentiate tumor radish lines 12, 19, and 21 from the others, and two which differentiate line 6.
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