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Immunospecific nuclear proteins of colorectal tumors

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Szymczyk P., Krajewska W. M., Jakubik J., Berner A., Berner J., Brocki M., Rajczyk B., Kilianska Z.

Cellular and Molecular Biology Letters

1996

tom 01

nr 4

Electrophoretically specific nuclear proteins of human colon adenocarcinoma with mol. wt of 35-40 kDa were used as immunogen to produce rabbit antiserum. Expression of cancer-specific antigens was investigated by Western blot technique among nuclear proteins from normal and cancerous mucosa. Obtained antiserum crossreacted mainly with 36 kDa polypeptide in 23 of 26 (88,5%) colorectal tumor nuclear fractions but not with any of normal ones. It was also observed that this antiserum recognized 36 kDa antigen in 10 of 12 and 6 of 7 nuclear fractions from other cancers, ie. gastric and lung, respectively. In part of studied tumors antiserum crossreacted also with the antigens of 38 and 32-33 kDa. Expression of 36 and 32-33 kDa components seems to be correlated with colorectal cancer progression from A to B stage of disease according to the classification of Dukes. Immunoblot analysis revealed that cancer-specific 36 kDa polypeptide, mainly associated with nuclear compartment, can be also detected within 10P and 100P fractions of colorectal tumors.

Ograniczanie wyników

1 Cellular and Molecular Biology Letters

1 Berner A.

1 Berner J.

1 Brocki M.

1 Jakubik J.

1 Kilianska Z.

1 Krajewska W. M.

1 Rajczyk B.

1 Szymczyk P.

1 1996

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Immunospecific nuclear proteins of colorectal tumors