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Some nutritional constituents in bulbs of selected Allium cultivars

100%

Leja M., Kołton A., Kaminska I., Wyzgolik G., Matuszak W.

Folia Horticulturae

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2008

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tom 20

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nr 2

2

Changes in lactate, pyruvate and glucose levels in field hockey players under different training and match stimuli

100%

Podgorski T., Adrian J., Nowak A.

Trends in Sport Sciences

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2015

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tom 22

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nr 3

Introduction. Lactate, pyruvate and glucose levels are the most common biochemical markers used for controlling training loads and physical efficiency of athletes. Each stage of a field hockey training cycle requires activation of a different metabolite responsible for physical exercise. Aim of Study. The aim of the study was to determine the biochemical response of field hockey players to different types of exercise in a training cycle in comparison to a real match. Material and Methods. Ten male university team field hockey players took part in the study. The players were examined six times during an annual training cycle. The examination consisted of the following tests: treadmill test (twice), 13 km running, interval training, spinning, and a real field hockey match. During the tests preand post-exercise capillary blood from a fingertip was collected to determine the lactate (La), pyruvate (Pa) and glucose levels using enzyme methods. Furthermore, bioelectrical impedance analysis (BIA) was carried out four times during the annual training cycle. Results. Each exercise test increased the La and Pa concentrations, however, the glucose level was raised only during the treadmill tests. The 13 km running test and interval training results were statistically different. The most essential changes of La and Pa concentrations were noted between the treadmill tests and the match. Conclusions. The aim of field hockey training is to prepare players to meet match requirements. The analysis of players’ metabolic responses to different kinds of training indicates that a match effort is similar to 13 km running and physiologically close to interval training

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Muscle biochemistry in rainbow trout Oncorhynchus mykiss following Yersinia ruckeri vaccination

80%

Tkachenko H., Grudniewska J., Pekala A.

Baltic Coastal Zone. Journal of Ecology and Protection of the Coastline

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2016

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tom 20

Vaccination of rainbow trout against Yersiniosis confers a high degree of protection to the fish (Raida et al. 2011). On the other hand, vaccination could alter metabolitic reactions in organisms. Therefore, exploring the effects of vaccination against Y. ruckeri on health condition of trout in general, and oxidative stress biomarkers and biochemical alterations in different tissues specifically, would be valuable. This prompted us to investigate theeffects of vaccination against Y. ruckeri on muscle function, and the oxidative mechanism underlying those effects, by detecting relevant lipid peroxidation (2-thiobarbituric acid reactive substances, TBARS) and protein oxidation biomarkers (aldehydic derivatives and ketonic derivatives) as well as biochemical alterations (aminotransferases and lactate dehydrogenase activity, lactate and pyruvate levels) in rainbow trout Oncorhynchus mykiss following Y. ruckeri vaccination at first month after oral immunization. Concentrated vaccine with inactivated by formalin Y. ruckeri strains was enclosed by fish feed, and was administered three times every other day. Rainbow trout from each group were euthanized 30 days after the immunization, and then muscle tissue were sampled for analysis. The TBARS level in the muscle tissue of vaccinated group was at same level compared to unhandled group. The ketonic derivatives of oxidatively modified proteins in the trout following Y. ruckeri vaccination at first month after immunization were significantly increased compared to the level in the controls, while the aldehydic derivatives of oxidatively modified proteins were non-significantly increased. Pyruvate level was increased by 47% (p = 0.013) in vaccinated trout compared to values of untreated fish. Lactate level, aminotransferases and lactate dehydrogenase activities were nonsignificantly altered in vaccinated trout. Our results suggest that vaccination could promote the activation of the gluconeogenic substrate-providing enzymes, as well as substrates for aerobic metabolism that might in turn contribute to increase of oxidatively modified proteins. The oxidative stress biomarkers, i.e. content of oxidative protein damage, as well as biochemical enzymes and substrates were sensitive to vaccination of trout against Y. ruckeri and may potentially be used as biomarkers in evaluating vaccine toxicity in rainbow trout. From a practical point of view, the results may be useful in relation to studies of infections and the development, administration and uptake of new vaccines applicable for large amounts of fish.

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Exercise-induced biochemical alterations in the blood of horses involved in recreational horseback riding in Pomeranian Region (Northern Poland)

80%

Tkachenko H., Witaszek M., Pazontko-Lipinski P.

Baltic Coastal Zone. Journal of Ecology and Protection of the Coastline

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2018

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tom 22

The aim of our study was to determine changes in some biochemical indices (alanine (ALT) and aspartate aminotransferase (AST), lactate dehydrogenase (LDH)) as well as lactate and pyruvate level in the blood of horses exercised in recreational horseback riding from Pomeranian regions during a training session. Measurement of values of liver biomarkers (AST, ALT) and muscle damage indicator (LDH), followed by a variety of training programs, can help to better understand the acute and chronic effects of resistance training. Thirteen healthy adult horses from central Pomeranian region in Poland (village Strzelinko, N54°30'48.0" E16°57'44.9"), aged 9.5±2.4 years old, were used in this study. All horses participated in recreational horseback riding. Training started at 10.00 AM, lasted 1 hour and consisted from a ride of cross country by walking (5 min), trotting (15 min), walking (10 min), trotting (10 min), walking (5 min), galloping (5 min), and walking (10 min). Blood was drawn from jugular veins of the animals in the morning, 90 minutes after feeding, while the horses were in the stables (between 8.30 and 10.00 AM), and immediately after exercise session (between 11.00 AM and 2.00 PM). Blood was stored in tubes with K3-EDTA and sodium citrate (3.8%) and held on ice until centrifugation at 3,000 rpm for 15 minutes. The plasma was removed. Plasma was used for the determination of aminotransferases and lactate dehydrogenase activity; whole blood was used for determination of lactate and pyruvate level. The regular training lead to adaptive processes which provoke changes in biochemical indices. In our research, non-significant alterations of AST and LDH activities in horses involved in recreational horseback riding were observed. This may indicate a normal course of aerobic-anaerobic glycolysis in horses involved in recreational horseback riding during a training session. Moreover, ALT activity was decreased by 20.6% (p = 0.000) during a training session. Increased blood lactate level in horses involved in recreational horseback riding during training session could be explained by increasing lactate formation via the reduction of pyruvate catalyzed by LDH as a result of anaerobic energy supply. Based on these results, it is concluded that the endurance exercises lead to specific metabolic changes accompanied by a redistribution of energy supply for improving resistance to exercises and athletic performance of horses. Therefore, the present data can be useful to assess the status of athletes and the degree of their training adaptability providing an opportunity to modify the training schedule to achieve the desired performance.

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Nonphosphorylated D-gluconate pathway in Aspergillus terricola

80%

Elsayed M. A.

Acta Microbiologica Polonica

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1999

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tom 48

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nr 1

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Isoprenoid biosynthesis via 1-deoxy-D-xylulose 5-phosphate-2-C-methyl-D-erythritol 4-phosphate [DOXP-MEP] pathway

80%

Wanke M., Skorupinska-Tudek K., Swiezewska E.

Acta Biochimica Polonica

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2001

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tom 48

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nr 3

Higher plants, several algae, bacteria, some strains of Streptomyces and possibly malaria parasite Plasmodium falciparum contain the novel, plastidic DOXP/MEP pathway for isoprenoid biosynthesis. This pathway, alternative with respect to the classical mevalonate pathway, starts with condensation of pyruvate and glyceral- dehyde-3-phosphate which yields 1-deoxy-D-xylulose 5-phosphate (DOXP); the latter product can be converted to isopentenyl diphosphate (IPP) and eventually to isoprenoids or thiamine and pyridoxal. Subsequent reactions of this pathway involve transformation of DOXP to 2-C-methyl-D-erythritol 4-phosphate (MEP) which after condensation with CTP forms 4-diphosphocytidyl-2-C-methyl-D-erythritol (CDP-ME). Then CDP-ME is phosphorylated to 4-diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate (CDP-ME2P) and to 2-C-methyl-D-erythritol-2,4-cyclodiphosphate (ME-2,4cPP) which is the last known intermediate of the DOXP/MEP pathway. For-mation of IPP and dimethylallyl diphosphate (DMAPP) from ME-2,4cPP still requires clarification. This novel pathway appears to be involved in biosynthesis of carotenoids, phytol (side chain of chlorophylls), isoprene, mono-, di-, tetraterpenes and plastoquinone whereas the mevalonate pathway is responsible for formation of sterols, sesqui- terpenes and triterpenes. Several isoprenoids were found to be of mixed origin suggesting that some exchange and/or cooperation exists between these two pathways of different biosynthetic origin. Contradictory results described below could indicate that these two pathways are operating under different physiological conditions of the cell and are dependent on the developmental state of plastids.

Ograniczanie wyników

2 Baltic Coastal Zone. Journal of Ecology and Protection of the Coastline

1 Acta Biochimica Polonica

1 Acta Microbiologica Polonica

1 Folia Horticulturae

1 Trends in Sport Sciences

2 Tkachenko H.

1 Adrian J.

1 Elsayed M. A.

1 Grudniewska J.

1 Kaminska I.

1 Kołton A.

1 Leja M.

1 Matuszak W.

1 Nowak A.

1 Pazontko-Lipinski P.

1 Pekala A.

1 Podgorski T.

1 Skorupinska-Tudek K.

1 Swiezewska E.

1 Wanke M.

1 Witaszek M.

1 Wyzgolik G.

1 2018

1 2016

1 2015

1 2008