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BamHI restriction fragment length polymorphism within the env gene of bovine leukaemia provirus

100%

Markiewicz L., Kaminski S., Rulka J.

Bulletin of the Veterinary Institute in Puławy

2004

tom 48

nr 1

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Modified procedure for PCR detection of proviral DNA of bovine leukaemia virus

100%

Czarnik U., Kaminski S., Rulka J., Walawski K.

Bulletin of the Veterinary Institute in Puławy

2000

tom 44

nr 2

PCR modification was performed by using two sets of primers specific for BLV gag gene and bovine kappa-casein gene (CASK) which show the possibility of disclosing BLV misdiagnosis. The results obtained indicate that the described PCR modification can be proposed as a simple and reliable method of BLV provirus detection.

PCR in the diagnosis of bovine leukaemia virus infection

67%

Kubis P., Rulka J., Kur J., Dabrowski S.

Bulletin of the Veterinary Institute in Puławy

1996

tom 40

nr 2

Whole blood samples from 73 animals from a dairy herd infected with BLV were used for detection of proviral DNA by means of PCR technique. At the beginning 24 cows were examined with primer set ZM1/ZM2. Seven out of 13 ELISA-positive animals –were PCR-positive too and 3 were PCR-positive among 11 serologically negative. Due to unsatisfactory results the rest of the samples were tested with a second primer set ZM2/ZM3. In this study 20 cows out of 25 ELISA-positive were PCR-positive and 8 out of 24 seronegative cows were PCR-positive. The findings of seronegative PCR-positive and seropositive PCR-negative cattle indicate that further work is needed. We plan to use the nested PCR because this technique seems to be more specific and sensitive.

Ograniczanie wyników

3 Bulletin of the Veterinary Institute in Puławy

3 Rulka J.

2 Kaminski S.

1 Czarnik U.

1 Dabrowski S.

1 Kubis P.

1 Kur J.

1 Markiewicz L.

1 Walawski K.

1 2004

1 2000

1 1996

Wyniki wyszukiwania

BamHI restriction fragment length polymorphism within the env gene of bovine leukaemia provirus

Modified procedure for PCR detection of proviral DNA of bovine leukaemia virus

PCR in the diagnosis of bovine leukaemia virus infection