Wyniki wyszukiwania

1

Characterization of the protein gp1 from bacteriophage phiIN93

100%

Matsushita I., Yanase H.

Biological Letters

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2011

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tom 48

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nr 1

2

Distribution of transcription factor binding sites along 5'-upstream sequences of genes: estimation of minimal promoters and upstream regulatory regions

100%

Malewski T., Glazko G., Sazanov A.

Biotechnologia

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2004

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nr 3

3

Genetic distances in hens estimated with protein genes frequencies and procedures of DNA analysis

100%

Brodacki A., Tarkowski J., Flis J.

Electronic Journal of Polish Agricultural Universities. Series Animal Husbandry

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2003

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tom 06

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nr 2

Egg yolk and white proteins from hens kept in eight conservation flocks in Poland were separated with horizontal polyacrylamide gel electrophoresis. The analyses were done on 100-120-egg samples from each of the following breeds: Green-Legged Partridge, ZKF and ZK11, Yellow-Legged Partridge, Z33, Polbar, PB, Leghorn, H22 and G99, Rhode Island Red, RD2, and Sussex, S66. After estimating phenotype frequency based on 30-35 bands on the electrophoregrams, the following were identified: gene frequencies of fast-migrating prealbumins, Pa-F, and transferrins, TF, in egg yolk, as well as ovalbumins Ov-A, ovoglobulins, G3, G4, G2, and conalbumins, Co, in the white of egg. Similarity and Nei's genetic distances were calcula-ted according to gene frequency (GF) and procedures used in analysing DNA polymorphism, based on frequencies of particular protein bands (BF) and band sharing (BS). Application of GF and BF in the evaluation of genetic distances gave similar results.

4

Expression of human membrane skeleton protein genes for protein 4.1 and betaIIsigma2-spectrin assayed by real-time RT-PCR

100%

Taylor-Harris P. M., Felkin L. E., Birks E. J., Franklin R. C. G., Yacoub M. H., Baines A. J., Barton P. J. R., Pinder J. C.

Cellular and Molecular Biology Letters

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2005

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tom 10

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nr 1

5

Analysis of the fragment of capsid protein gene sequences of Polish strains of European brown hare syndrome virus

100%

Chrobocinska M.

Bulletin of the Veterinary Institute in Puławy

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2002

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tom 46

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nr 2

6

Single nucleotide polymorphism database of candidate genes associated with cow milk protein biosynthesis

100%

Kaminski S., Rusc A., Malewski T.

Journal of Animal and Feed Sciences

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2004

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tom 13

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nr 1

7

The DNA-binding capacity of genetic variants of the bovine STAT5A transcription factor

80%

Flisikowski K., Szymanowska M., Zwierzchowski L.

Cellular and Molecular Biology Letters

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2003

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tom 08

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nr 3

The STATs are a family of transcription factors. STAT5A, previously known as MGF, transduces prolactin signals to the milk-protein genes. Here, we describe the detection of nucleotide sequence polymorphism in exon 16 of the bovine STAT5A gene, coding for the SH2 domain. SSCP was found in a 281-bp PCR amplified gene fragment, lying between positions 12,525 and 12,806, and encompassing parts of intron 15 and exon 16 of the bovine STAT5A gene (GenBank AJ 237937). Three SSCP patterns (genotypes) were identified in a group of 108 animals of different cattle breeds. The DNA sequencing showed that they differed by a CCT deletion at position from 12,549 in intron 15, and a T→C substitution at position 12,743 in exon 16. The latter mutation changes an amino acid sequence in the STAT5A protein - a Val/Ala substitution at position 686. Since T→C substitution creates a new Msl1 site, genetic variants in the bovine STAT5A gene can be distinguished with RFLP analysis. The frequency of alleles T and C varied between the different cattle breeds studied; the CC genotype was the least frequent and the frequency of alleles T and C was 0.842 and 0.158, respectively. Proteins were extracted from the cell nuclei of liver tissues derived from bulls of different STAT5A genotypes and subjected to EMSA in order to study if the amino acid substitution might change the DNA-binding capacity of STAT5A transcription factor. Statistically significant (p<0.05) differences in nuclear protein binding to DNA were observed between genotypes TT and CC; nuclear proteins derived from CC animals always showed less DNA protein complexing than those of TT animals. EMSA competition experiments confirmed that STAT5 transcription factors take part in the formation of the DNA-protein complexes.

8

Nucleotide sequence polymorphism within exon 4 of the bovine prolactin gene and its associations with milk performance traits

80%

Brym P., Kaminski S., Wojcik E.

Journal of Applied Genetics

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2005

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tom 46

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nr 2

Prolactin plays an important regulatory function in mammary gland development, milk secretion, and expression of milk protein genes. Hence the PRL gene is a potential quantitative trait locus and genetic marker of production traits in dairy cattle. We analysed the sequence of the PRL gene to investigate whether mutations in this sequence might be responsible for quantitative variations in milk yield and composition. Using SSCP and direct sequencing, we detected six single-nucleotide polymorphisms within a 294-bp prolactin gene fragment involving exon 4. All detected mutations were silent with respect to the amino acid sequence of the protein. PCR-RFLP genotyping of SNP 8398 R (Rsal) was used to assess allele frequencies in 186 Black-and-White cows (0.113 and 0.887 for A and G, respectively) and in 138 Jersey cows (0.706 and 0.294 for A and G, respectively). Black-and-White cows with genotype AG showed the highest milk yield, while cows with genotype GG showed the highest fat content.

9

The 14-3-3 protein binds to the nuclear matrix endonuclease and has a possible function in the control of plant senescence

80%

Markiewicz E., Wilczynski G., Rzepecki R., Kulma A., Szopa J.

Cellular and Molecular Biology Letters

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1996

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tom 01

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nr 4

The nuclear matrices of plant cell nuclei display an intrinsic nuclease activity which is capable of nicking supercoiled DNA. Recently a cDNA encoding the 14-3-3 protein from Cucurbita pepo has been cloned and sequenced. The evidence that the recombinant 14-3-3 protein associates with DNase I and endogenous plant nuclease is presented. Evidence is also presented that the cloned 14-3-3 protein isoform, unique in its binding to nuclease within the 14-3-3 family, is located in the nuclei and in the nuclear matrix. Transgenic potato plants were created where the 14-3-3 protein derived from Cucurbita was overexpressed. An increase in tuber number and a decrease in tuber size in the transformants was also observed. The adenine nucleotide pool in leaves of transgenic plants was significantly reduced and they contain more chlorophyll and loose it slower when grown in the dark. A decrease in 14-3-3 protein content concomitant with an increase in nuclease activity in senescent plants was detected and this was markedly delayed in transgenic potato plants which overexpressed the 14-3-3 protein. It is proposed that a function of the isolated 14-3-3 isoform is the control of the nuclease activity and hence senescence.

Ograniczanie wyników

Characterization of the protein gp1 from bacteriophage phiIN93

Distribution of transcription factor binding sites along 5'-upstream sequences of genes: estimation of minimal promoters and upstream regulatory regions

Genetic distances in hens estimated with protein genes frequencies and procedures of DNA analysis

Expression of human membrane skeleton protein genes for protein 4.1 and betaIIsigma2-spectrin assayed by real-time RT-PCR

Analysis of the fragment of capsid protein gene sequences of Polish strains of European brown hare syndrome virus

Single nucleotide polymorphism database of candidate genes associated with cow milk protein biosynthesis

The DNA-binding capacity of genetic variants of the bovine STAT5A transcription factor

Nucleotide sequence polymorphism within exon 4 of the bovine prolactin gene and its associations with milk performance traits

The 14-3-3 protein binds to the nuclear matrix endonuclease and has a possible function in the control of plant senescence