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This paper describes laparoscopy-guided prostate biopsy experiments in 13 dogs. Biopsy material was evaluated histopathologically to diagnose causes of prostatic gland enlargement. Laparoscopic biopsy was performed in male dogs diagnosed with prostatic gland enlargement, based on clinical symptoms and results of rectal examinations.
The aim of the study was to evaluate the effect of two different methods of partial prostatectomy, to evaluate the influence of 96° ethanol injection on hyperplastic prostate and to compare the efficacy of ethanol injection with the efficacy of surgical treatment of the prostate. The study was performed in the years 20012003 in the surgical department of Dr. L. Kriauèeliûnas Small Animal Clinic. The surgical treatment (prostatectomy) was performed on 8 dogs using 2 methods: fillet and subcapsular. We compared the effectiveness and postoperative hystological evaluation of bioptats one and two weeks after surgery. We have performed ethanol injection on 5 dogs and evaluated the histological feature of prostatic tissues after injection to measure the effect of ethanol. The fillet method is technically simpler, shorter and the tissues of the prostate heal more quickly. Ethanol injection induces multi-focular coagulative necrosis, followed by fibrosis and atrophy of the prostate. This way of treatment is simpler and easier because there are no sections through prostate tissues. To avoid a section of the abdominal wall, a transurethral catheter can be used for the ethanol injection.
The aim of this study was to investigate the influence of standardized Epilobium angustifolium L. extract [100 mg/kg/day, p.o.] on the expression level of 5α-reductase type 2 (Srd5ar2) mRNA and Mapk3 mRNA a representative of non-genomic xenobiotics signaling pathway. It was shown that plant extract from the E. angustifolium showed a slight tendency to reduce prostate weight in hormonally induced animals (p>0.05) and in testosterone induced animals receiving both, extract and finasteride (p<0.05). Finasteride in rats induced by testosterone caused a smaller decrease in the level of mRNA 5α-steroid reductase 2 (SRd5ar2), than in rats treated with the hormone and studied plant extracts. In general, an increase in the amount of MAPK3 mRNAs in testosterone-induced groups of rats receiving tested plant extract with or without finasteride was observed, while the expression of type 2 5α-steroid reductase decreased (p<0.05). Further experimental studies should be performed in order to understand the molecular basis of interactions, the efficacy and safety of tested plant extracts.
The aim of this study was to investigate the influence of standardized crude aqueous Epilobium angustifolium L. extract [100 mg/kg/day, p.o.] on the expression level of SRC kinase mRNA - a representatives of non-genomics xenobiotics signaling pathway in prostate ventral lobes of testosterone-induced, castrated rats. We have shown that in all analyzed groups induced by testosterone an elevation of SRC kinase mRNA transcription was observed, in comparison to control animals (not receiving the testosterone), (p<0.05). Finasteride in rats induced by testosterone caused the strongest inhibition of SRC mRNA transcription (p<0.05). In rats receiving testosterone and the plant extract a ca. 90% decrease of mRNA level was observed vs. testosterone-induced animals (p<0.05), while in testosterone-induced animals receiving concomitantly E. angustifolium extract and finasteride the observed reduction reached 87.3% (p<0.05). We did not observed, however, any positive feedback between studied plant extract and finasteride in the inhibitory activity (p<0.05). Further experimental studies should be performed in order to the understanding the molecular basis of interactions, the efficacy and safety of tested plant extract.
Porphyrin photosensitizers tend to localize in mitochondria. The depolarization of mitochondrial membrane is one of the early stages of apoptosis and Laser Scanning Fluorescence Microscopy allows to determine changes in transmembrane mitochondrial potential under influence of PDT depending on the kind of photosensitizer (PP(Arg)2, Hp(Arg)2), the energy dose (5, 10, 30 and 50 J/cm2) and time periods (24 and 48 hours after irradiation) in the LNCaP (lymphonodal metastasis of prostate carcinoma, the androgen dependent cell line). Cyototoxicity induced by PP(Arg)2- and Hp(Arg)2-based PDT depending on energy dose and time after irradiation in prostate carcinoma is determined with MTT. Generally, it was shown that lower energy doses induce greater changes in transmembrane mitochondrial potential. Hp(Arg)2-based PDT was more effective causing greater mitochondrial membrane depolarization and cell viability decrease in comparison to PP(Arg)2-mediated PDT (in the case of maximal nontoxic photosensitizer doses used).
The objective of the present research was to assess the usability of three diagnostic imaging modalities for canine prostate gland evaluation. The studies included 33 male dogs of various breeds. A radiographic diagnostic procedure was employed as well as dual modality ultrasound imaging, i.e. transabdominal (TAUS) and transrectal (TRUS). Radiograms were studied to evaluate the prostate gland size and localisation. Both ultrasound imaging techniques were compared regarding the imaging of various prostate parenchyma areas. It was found that a suitable USG modality should be tailored to the prostate size and localisation. The TRUS technique proved to be most helpful in visualising the gland localised in the pelvic cavity, while the TAUS scanning for the enlarged, displaced cranially prostate.
Cathepsin D is a cysteine endopeptidase that belongs to the lysosomal enzyme family. The aim of the study was to evaluate the enzyme immunoexpression and activity in selected male genital organs in mature Wistar rats. The activity of cathepsin D was measured spectrophotometrically in homogenates of the testis, epididymis, seminal vesicle and prostate. Immunohistochemical staining was also performed in the ductus deferens. Enzyme activity was found in the following sequence: testis>epididymis>dorsal prostatic lobe>seminal vesicle>lateral prostatic lobe>ventral prostatic lobe. Although there were differences in enzyme activity between various organs of the male reproductive system, cathepsin D immunoreactivity was seen exclusively in the Sertoli and Leydig cells in the testis.
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WWOX, the tumour suppressor gene affected in multiple cancers

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WWOX is a tumour suppressor gene affected in multiple cancers, especially in breast, prostate and ovary. This gene is located at the chromosomal area 16q23.3-24.1, which was identified as a common chromosomal fragile site FRA16D. WWOX turned out to possess tumour suppressor features despite the fact that the most basic (classical) way of tumour suppressor gene inactivation involves both alleles (e.g. through deletions, point mutations and promoter methylation), which is very rare event in a case of WWOX, occurring only in few cell lines. A large number of papers corroborate the phenomenon of correlation between the loss of WWOX expression and more aggressive/worse prognosis in many different types of tumours, for example breast cancer, non-small cell lung cancer, bladder cancer, gastric cancer or sporadic meningiomas. Ectopically increased WWOX expression promotes migration through basal membrane, however suppresses anchorage independent growth and induces normal-like colony formation in matrigel.
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