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The purpose of the study to assess the protective value of different vaccines applied in the prophylaxis of swine colibacillosis under field conditions. The following were used: 1) Porcoli LT vaccine containing the purified K88 ab, K88 ас, K99 and 987 P pili plus the LT component; 2) Enterit 1 — ie a suspension of E. coli strains with fimbrial antigens (K88, K99, F41, 987 P) supplemented with aluminium hydroxide as an adiuvant; 3) Enterit 2 vaccine, in a suspension of E. coli strains containing the above mentioned fimbrial antigens plus two attenuated rotaviruses of A and В serotypes; 4) Enterit 3 — a polyvalent vaccine composed of the mentioned rotaviruses, an attenuated strain of TGE virus and a suspension of E. coli strains with fimbrial antigens; 5) Colivac S — inactivated culture of immunogenic E. coli strains, isolated in Poland, and aluminium hydroxide as adiuvant. The experiments were performed in two large farms. Laboratory examinations revealed that diarrhoea in suckling piglets was caused by a mixed infection of E. coli and pig rotaviruses. The vaccines were applied after according to the manufacturer’s recommendations. The safety and protective values of individual vaccines were determined using the following criteria: the clinical state of pigs and gilts after vaccination, the number of piglets per litter, the number of litters with the signs of diarrhoea (at least in 5 piglets) by the 28th day of their lives, mean body weight gains in a piglet during 28 days of its life, number of weaned piglets and the percentage of death from birth to weaning. It was demonstrated that in all groups of experimental animals diarrhoea occurred less frequently than in the control group. The best results were obtained in pigs immunized with Enterit 2 and Porcoli LT vaccines. The number of diarrhoeal litters in those animals was 19,1 per cent and 20,8 per cent in sows, and 23,5 and 25,4 per cent in gilts. The respective data in control groups were 60 and 83,3 per cent. A decreased morbidity resulted in better body weight gains in experimental groups during the frist 28 days of their lives. The best results were found in piglets born by sows and gilts immunized with Porcoli LT vaccine — 5549 g in pigs born by sows and 5392 g in suckling piglets born by gilts. The respective values for the vaccine Enterit 2 were 5474 g and 5341 g. In control groups these values wer 4942 g and 4125 g. The vaccination decreased the number of deaths in the preweaning period. In litters produced by vaccinated sows the number of weaned piglets was higher from 0,6 to 1,6 piglets than that in control groups.
Celem badań było wypróbowanie kilku prostych metod pośredniej stymulacji środowiska przeciwdziałających nudzie oraz ograniczeniu zachowań agresywnych u prosiąt. Z zastosowanych przedmiotów największe zainteresowanie wzbudził łańcuch. Stosowanie zabawek wpłynęło na nieznaczne obniżenie dziennych przyrostów, przypuszczalnie na skutek zwiększonego zużycia energii na zabawę.
The aim of present study was to determine α and β toxin expression as well as the prevalence of α, β, ε, ι, β2 toxin genes and the enterotoxin gene of Clostridium perfringens strains isolated from suckling piglets with diarrhea. Rectal swabs, feces samples and intestine sections originating from 15 farms were examined during the study. All isolated through ELISA Cl. peringen strains demonstrated β toxin expression and none of them demonstrated β toxin. All isolates were positive for the β and negative for the β, ε, ι and enterotoxin gene, implying that only non-enterotoxigenic type A strains were detected. Over 41% of the isolates demonstrated β2 toxin gene. The β2 positive strains were isolated from samples originating from 9 farms. The lack of type C strains among isolates collected during the study was also noted, as well as the different intensity of pathological changes in the intestines of piglets from which β2 positive strains were isolated.
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