Wyniki wyszukiwania

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Prokaryotes from different phylogenetic groups in surface microlayer and subsurface water in a eutrophic lake

100%

Burkowska A., Walczak M.

Polish Journal of Environmental Studies

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2013

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tom 22

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nr 4

Prokaryotes from different phylogenetic groups were studied in surface microlayer (SM, up to 100 μm) and subsurface water (SW – 20 cm) in a eutrophic lake over three months (July, August, and October). The abundance of prokaryotes was determined by epifluorescence microscopy after DAPI staining, and phylogenetic diversity was determined by fluorescence in situ hybridization (FISH) with group-specific, fluorescently labeled oligonucleotide probes. In SW bacteria made up most of the entire community of DAPI-stained microorganisms (54-69%) and in SM bacteria made up only 33-44% of DAPI-stained microorganisms. Archaea corresponded to a small fraction of both bacterioneuston and bacterioplankton. The counts of Archaea and bacteria were significantly higher in SW than in SM. Among all proteobacteria included in the research, γ-proteobacteria represented the most abundant fraction: 42-72% in SM and 39-61% in SW. Statistical analysis revealed that the abundance of γ-proteobacteria is positively correlated with temperature and with dissolved oxygen. β-proteobacteria were the least abundant fraction.

2

Spectrin-like protein in cells of Chlamydomonas reinhardtti and Escherichia coli

100%

Bisikirska B., Hanus-Lorenz B., Lorenz M., Strzalka K., Sikorski A. F.

Biophysics of Membrane Transport

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1994

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tom 12

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nr 2

3

Mycoplasma-induced genome rearrangements in plants

100%

Chernov V. M., Gogolev Y. V., Popova N. V., Chernova O. A.

Biological Bulletin of Poznań. Supplement

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1997

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tom 34

4

Recombinant ferritin protects mice against challenge with Echinococcus granulosus?

80%

Wang Y., Li Z., Li Z., Bo Y., Zhao W.

Acta Parasitologica

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2009

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tom 54

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nr 4

335-340

Ferritin is well known as the main intracellular iron storage protein in both prokaryotes and eukaryotes, keeping it in a soluble and non-toxic form, though the role of ferritin as a vaccine candidate in echinococcosis has not yet been delineated. Through our study, ferritin was cloned from Echinococcus granulosus and expressed in Escherichia coli. The recombinant E. granulosus ferritin (rEgferritin) has a molecular weight of 19 kDa and could be recognized by anti-mice serum in Western blotting. The specific antibody was detected by enzyme-linked immunosorbent assay. Mice vaccinated with rEgferritin and challenged intraperitoneally with E. granulosus protoscoleces revealed significant protective efficacy up to 85.6%, compared with the control group. Thus, rEgferritin could be a promising candidate as an effective vaccine to prevent the infection of echinococcosis.

5

Identification of microsatellite markers in the rye genome

80%

Bolibok H., Rakoczy-Trojanowska M., Wyrzykowska M., Radecka M., Orczyk W.

Cellular and Molecular Biology Letters

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2006

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tom 11

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nr 2

The rye genomic library, which consists of DNA fragments in the range of 0.5–1.1 kb, was screened for the presence of tri-and tetranucleotide and compound microsatellites. Of the 1,600,000 clones analysed, 102 clones were positive and 41 were suitable for SSR primer pair design. Twenty-six primer pairs amplified specific products, and six of them were capable of detecting polymorphism among 30 rye accessions of different genetic backgrounds. Using a set of Chinese Spring-Imperial wheat-rye addition lines, it was possible to locate 3 newly identified microsatellites on chromosomes 3R, 4R and 7R.

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Wystepowanie promieniowcow w ryzosferze wybranych gatunkow traw uprawianych w mieszance z koniczyna biala na intensywnym pastwisku gorskim

61%

Bis H.

Acta Agraria et Silvestria. Series Agraria

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1992

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tom 30

3-13

Z badań mikrobiologicznych gleb, pochodzących z ryzosfery pięciu gatunków traw: Dactylis glomerata. Phleum pratense, Festuca arundinacea, Festuca rubra i Lolium perenne, uprawianych w mieszance z 30% udziałem koniczyny białej (indywidualnie i łącznie), na intensywnym pastwisku górskim wynika, że w badanych środowiskach glebowych dominują promieniowce z rodzaju Streptomyces. Badane trawy różnicują liczebność i skład gatunkowy promieniowców. Częstotliwość występowania poszczególnych gatunków promieniowców uległa znacznym zmianom w okresie trwania doświadczenia.

Ograniczanie wyników

Prokaryotes from different phylogenetic groups in surface microlayer and subsurface water in a eutrophic lake

Spectrin-like protein in cells of Chlamydomonas reinhardtti and Escherichia coli

Mycoplasma-induced genome rearrangements in plants

Recombinant ferritin protects mice against challenge with Echinococcus granulosus?

Identification of microsatellite markers in the rye genome

Wystepowanie promieniowcow w ryzosferze wybranych gatunkow traw uprawianych w mieszance z koniczyna biala na intensywnym pastwisku gorskim