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Physical methods of microalgal biomass pretreatment

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The prospect of depletion of natural energy resources on the Earth forces researchers to seek and explore new and alternative energy sources. Biomass is a composite resource that can be used in many ways leading to diversity of products. Therefore, microalgal biomass offers great potential. The main aim of this study is to find the best physical method of microalgal bio- mass pretreatment that guarantees efficient lipid extraction. These studies identifies biochemical composition of microalgal biomass as source for biodisel production. The influence of drying at dif- ferent temperatures and lyophilization was investigated. In addi- tion, wet and untreated biomass was examined. Cell disruption (sonication and microwave) techniques were used to improve lipid extraction from wet biomass. Additionally, two different extrac- tion methods were carried out to select the best method of crude oil extraction. The results of this study show that wet biomass after sonication is the most suitable for extraction. The fatty acid com- position of microalgal biomass includes linoleic acid (C18:2), palmitic acid (C16:0), oleic acid (C18:1), linolenic acid (C18:3), and stearic acid (C18:0), which play a key role in biodiesel production.
The article presents results of the research on the shrinkage of slightly degraded, freeze-dried archaeological oak-wood (Quercus sp.). Before drying, samples of wood were treated with 10, 20, and 30% water solutions of PEG 300, PEG 4000, and sucrose, as well as the mixture of both polyglycols. Dimensional changes in tangential, radial, and longitudinal directions were determined immediately after freeze-drying, and then, after seasoning of the freeze-dried samples in the air at RH 44 and 70%. Shrinkage of untreated and treated freeze-dried oak-wood was considerably smaller, than that of the material, which was dried naturally (ASET from 49 to 97%, ASER from 39 to 98%).
Irregular seed crop in Betula pendula (Silver Birch) is a reason to create seed reserves, ensuring nursery production in years of crop failure. This study investigated the effects of seed moisture content and storage temperature on germinability and seedling emergence in container cultivation. Mature catkins were collected separately from 3 trees. The mixture of winged nuts and scales was dried to 3 levels of moisture content and stored at 3°C, –3°C and –10°C. After storage for 3, 5 and 6 years, the seeds were separated from scales and next dewinged and sorted in acetone into empty and filled seeds. Cleaned nuts were germinated in the Jacobsen germinator. Besides, to assess seedling emergence, seeds were sown on the surface of peat mixed with perlite, in multi-cell trays under a plastic tunnel. Significant differences in germinability were detected depending on the mother tree, seed moisture content and storage time. The viability of seeds stored at –10°C remained unchanged for 6 years regardless of moisture content (ca. 8–12%). Seeds stored at higher temperatures lost their germinability faster. An unexplained increase in seedling emergence was observed after extended seed storage at –10°C, in contrast to a gradual decrease in seedling emergence after extended storage at –3°C.
Chilling stress is of major limiting factors influencing the growth and development of warm-season crops like cucumber. In this research, the possibility of chilling tolerance of cucumber seedlings was investigated through employing the drought and low-temperature pretreatments. The factorial experiment consisted of two factors including cucumber cultivars (i.e. ‘Super Dominos’ and ‘Super Star’) and hardening treatments (control, low temperatures at 10°C, and 15°C and drought simulated by 10% and 20% PEG) based on completely randomized design (CRD) in 3 replications. After applying treatments and providing them 48 h opportunity to be recovered, the seedlings were subjected to 3°C for a six-day period and 6 h for each day. All hardening treatments improved seedlings’ growth, chlorophyll content, total phenol (TP) and antioxidant enzyme activities, while reducing chilling injury index and malondialdehyde (MDA) content. Comparing to temperature hardening, the drought pretreatment showed to have a better effect on inducing the chilling tolerance into cultivars. Overall, the results of this experiment showed that employing drought and low-temperature pretreatments enabled cucumber seedlings to mitigate the harmful effects of chilling.
Celem pracy było przedstawienie wpływu wstępnego odwadniania osmotycznego jabłek odmiany Idared na ich właściwości rehydracyjne. Przed suszeniem konwekcyjnym stosowano osmotyczne odwadnianie jabłek w roztworze sacharozy o stężeniu 30 i 60% w temperaturze 30 i 50˚C przez 0,5, 1,0 i 1,5 h. Grubość próbek w kształcie prostopadłościanów o wymiarach 2,5 x 2,5 cm wynosiła 0,5 i 1,5 cm. Właściwości rehydracyjne suszu jabłkowego, analizowane na podstawie względnego przyrostu masy i ubytku suchej substancji, zależały od stężenia roztworu osmotycznego i początkowej grubości próbek. Niższe stężenie roztworu sacharozy oraz mniejsza grubość próbek wpływały na zwiększenie względnego przyrostu masy w suszach jabłkowych, ale różnice te były mniej widoczne w przypadku rehydracji prowadzonej przez 4 h w porównaniu z rehydracją 1-godzinną. Zmiany przyrostu masy suchej substancji i ubytku wody podczas wstępnego odwadniania osmotycznego jabłek przed suszeniem konwekcyjnym w małym stopniu korelowały ze względnym przyrostem masy suchej substancji w rehydrowanych suszach. Na wartości analizowanych wskaźników rehydracji większy wpływ niż przyrost masy suchej substancji miał stopień odwodnienia próbek wyrażony jako ubytek wody z jabłek.
This study was intendent to examine if N-acetylcysteine (NAC) changes liver GSH levels and influences ChE serum activity in rats intoxicated with chlorfenvinphos. The studies were conducted on male Wistar rats of 200±20 g body weight. One group of rats was pretreated with 0.1% water solution of NAC. NAC was administered in drinking water 24h before intoxication. The control groups received oil intragastrically by stomach tube in the amount of 0.1ml/100g (I), immediately or after NAC pretreatment. The experimental groups received oil solution of chlorfenvinphos in a dose of 0.02 LD50 or 0.1 LD50 immediately or after pretreatment with NAC. One and 24 hours after intoxication with chlorfenvinphos (or after NAC pretreatment) the blood samples were collected and livers were quickly removed and placed in iced 0.9% NaCl containing 0.16 mg/ml heparin. ChE serum activity and GSH level were measured. The results of this study demonstrated the changes in serum ChE activity and liver glutathione levels in the rats after administration of chlorfenvinphos at single doses. The results reported here indicate that NAC influences a decreased level of GSH in the liver of chlorfenvinphos-intoxicated rats and does not prevent ChE inhibition.
The aim of the work was to determine the level of dry matter, total nitrogen, and amino acids in two kinds of green dill parts (leaves or whole plants 25 cm in height) subjected to different pre-treatment before freezing (blanching or non-blanching) and stored after freezing for 12 months at temperatures of -20°C and -30°C. The leaves of dill contained more dry matter and nitrogen compounds than whole plants. Blanching reduced the content of dry matter and total nitrogen. Refrigerated storage (-20°C and –30°C) for 12 months did not change the content of dry matter and nitrogen compounds. No differences were observed between the leaves and whole plants in the content of total amino acids and sum of essential amino acids while significant differences appeared in the content of some amino acids. In comparison with the FAO/WHO standard of 1991 the level of essential amino acids was very high, lysine being a limiting amino acid.
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