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With immunofluorescence techniques large foci are visible in frozen sections of the tonsils and spleen of pigs infected with virulent strains of HCV. Low or moderate-virulent strains induce small, weakly fluorescent foci under the same conditions. This sometimes causes diagnostic problems. Therefore, under such conditions, isolation of virus in cell cultures should be applied for HC diagnosis. The culture might be examined for the presence of HCV by direct IFT using immunoenzymatic methods (PLA). The usefulness of the PLA for the diagnosis of HC was compared with IFT. Forty samples of tonsils, spleens and kidneys were collected from 10 pigs experimentally infected and from 5 field cases of HC. Isolation was performed on rapidly growing PK-15 cells seeded in flat bottomed microtitre plates. The cultures after 48 h were fixed and stained with hiperimmune porcine HC antiserum and after that with anti-porcine IgG-HRPO conjugate. The results were read microscopically using 50 X magnification. The results were considered positive if stained reddish-brown infected cells were detected. The applied immunoenzymatic method enables the detection of HCV in all samples, from experimentally infected pigs and field cases of HC. Using IFT, in some cases, it was difficult to determine exactly the results of examination.
Classical swine fever (CSF) is a worldwide economically important, highly contagious disease of swine. Poland has been free of this disease for the last 14 years. However, outbreaks occur in neighboring countries and create serious risks. Therefore, it seems essential to present the progress of laboratory diagnosis and vaccines. Since the CSF virus reservoir in wild boars is important for the infection of domestic swine, this topic has also been presented. The paper also describes diagnostic techniques for identifying the agents and serological tests for detecting the virus - it characterizes specific antibodies, including an assessment of their diagnostic value. Recent developments of CSF vaccines are discussed, including the live, lapinised vaccine, containing the attenuated Chinese C strain of CSF and marker vaccines, developed with the application of molecular biology and genetic engineering techniques. These vaccines, despite being less effective than the C-strain vaccine, enable to discriminate infected animals from vaccinated ones (DIVA strategy) when correspondent diagnostic kits are used. These detect antibodies which are exclusively characteristic for antigens of the vaccine strain or antibodies specific to antigens of the virulent CSF virus. Procedures of controlling CSF in wild boars were described including recommendations of diagnostic tests and oral vaccinations.
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