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The course of 20-year studies on the health of the population of Plock and its neighbourhood has been discussed as regards environmental pollution generated by refinery-petrochemical plant. Analysis of environmental factors and general principles of health-promoting phenomena were of particular concern. This paper demonstrates an increase in options of environmental factors in studies on health in ecologically polluted areas.
The research on the influence of pollution emitted by the WBM-30 bituminous mass factories on the winter wheat-crop was carried out in 1981. The factory was situated in the agricultural area far from the farm buildings in the village of Trzebownisko. The experimental area was divided into four zones (three zones of pollution and one control zone). The wheat ear samples were collected during full grain ripeness per 100 ears from different plantations in three repetitions in each zone. They showed that all examined features, such as, e.g. the average ear length and 1000 grain mass were substantially different between the 1st pollution zone and control zone.
Twelve morphometric variables of Cardaminopsis halleri individuals from four sites in upper silesia province were analyzed to establish the pattern of interpopulational relations. Two hundred plants were collected from two very polluted areas (near zinc-lead and zinc smelters) as well as from two other sites in unpolluted areas within the same bioclimatic region. stem size, dimensions of leaves and rosettes, as well as flower and seed numbers and in­dividual dry weights were studied using standard biometric methods. cluster analysis indicated overall differences among populations. Those four populations are different with respect to all variables. Discriminant function analysis confirmed seed number as the most important variable in the evaluation of interpopulational variability.
Analysis of endosperm development in plants from contaminated sites (vicinity of the Żelazny Most copper post-flotation reservoir in the Legnica-Głogów Copper District, and the zinc spoil in Katowice-Wełnowiec) showed general similarities in the pattern of endosperm formation in Echium vulgare, but also deviation from typical haustorium structure (~23% frequency), premature degeneration of the haustorium, or degeneration both of the haustorium and endosperm proper (~40%). The most significant irregularities of endosperm development included lower or higher number of nuclei in two cells of the lateral part of the endosperm, lower ploidy level of haustorium nuclei, and cellularization (instead of coenocytic structure) in the lateral cells of the endosperm. Irregularities and degenerative processes presumably resulted from the stress of environment conditions. Because of the nutritive function of those structures, degeneration or atypical structure of the endosperm and its haustorium in some maturing seeds may reduce the fertility of plants colonizing contaminated sites.
Preliminary observations of plants collected at a natural locality in Jany (Zielona Góra district, Poland) suggested that in some plants dyads occurred mixed with more or less regular tetrads, monads, triads and polyads. Similar results were obtained in plants growing on an unpolluted site at an experimental field in Modlnica near Cracow and in a highly polluted area close to a postfloatation reservoir at the Żelazny Most copper mine near Rudna (Silesia). However, in the plants growing in contaminated soil a higher degree of degeneration processes was observed. Either dyads or tetrads prevailed in the capitula in the analyzed plants. In some of their loculi, dyads and tetrads were mixed with monads, dyads, triads and/or polyads. Microcytes and pollen grains of different sizes were common. The sterility of mature pollen grain was slightly higher in a plant from Żelazny Most (80-85%) than in its derivative from Modlnica (65-75%). Degeneration of whole anthers in the plant from the polluted locality was frequent. In some anthers the destruction of meiocytes started early, together with precocious abortion of the anther tapetum.
Pods of some roadside plants such as Albizia lebbeck, Pongamia pinnata, Dalbergia sissoo, Leucaena leucncephala, Parkensonia aculeata, Sesbania sesban and Tecoma stans collected from the city area showed significant decreases in weight and length as compared to pods collected from a clean area. Azadirachta indica, Delonix regia and Peltophorum pterocarpum showed enhancement in pod weight or length. The seeds of most of the species collected from the polluted areas also showed significant reduction in germination as compared to the seeds of the control area, except Cassia surattensis and Tecoma stans. These two species showed a comparatively higher rate of germination in the polluted seeds. In this germination study the species were found to be resistant in the following order: C. surattensis, T. stans, A. lebbeck, P. pinnata, P. pterocarpum, S. sesban, A. indica, L. leucocephala, P. aculeata, and D. sissoo.
A method of determination of high Zn and Pb concentrations by means of EDXRF (energy-dispersive X-ray fluorescence spectrometry) is presented. Zn and Pb concentration in soil samples from contaminated areas ranged within a limit of Zn: 800–2000ppm and Pb: 200–600ppm. Soil analysis was conducted directly after the samples had been dried and powdered, as well as in situ in polluted areas. Due to the absence of the certificate reference materials for soils with such high Zn and Pb concentrations, samples of soil with the Pb and Zn amounts determined using the AAS method were used to perform calibration. The obtained detection limits are 30ppm and 19ppm for Zn and Pb, respectively. Because of the high analytes concentration and material heterogeneity, the repeatability of the results was examined according to the sample holder’s various positions in EDXRF analyzer; repeatability the of the results while putting into the sample holder and out of it, together with the repeatability of making the calibration. The results obtained using the EDXRF method were compared to these obtained using AAS and wavelength-dispersive X-ray fluorescence spectrometry (WDXRF).
The aim of this study was to examine the impact of Ca, Mg, Fe, Na, K, Zn, Cd, and Pb upon enzymatic activity of superoxide dismutase (SOD), catalase (CAT), and ceruloplasmine (CP) diurnal and nocturnal activity, and the content of thiobarbituric acid-reactive substances (TBARS), and their interaction with free radicals, in the blood of the White Stork (Ciconia ciconia), an altricial bird, during postnatal development, in polluted areas (copper manufacture) and in control environments. The age of chicks examined from an output from an egg was increased from 19 to 54 days. Samples of investigated wing venous blood were taken for AAS analyses of element concentration. We collected blood samples via veni-puncture of the brachial vein of chicks. We have stated significant interactions between Cd, Ca, and Mg, and TBARS, SOD, CAT, and CP activ­ity. Interaction with Fe, Na, K, Zn, and Pb were not significant. We observed regularities in the course of re­lationships in the case of Cd; interactions of Cd-enzyme activity were negative in the control environment, both during the day time and at night. The prevalence of cd participation in element-enzymes interaction takes place. Ca- and Mg-relationships were more differential; Ca-enzymes interactions were significant only during the day in polluted environments and all of them were positive. Relations with Mg were posi­tive during the day and negative at night, but significant in polluted areas only. We conclude that physiological activity of antioxidant systems SOD, CAT, and CP, and content of TBARS-active products are determined by concentrations of physiological elements and toxic heavy met­als. These groups of elements influenced enzymatic activity both through excess and deficiency of their concentration in the environment. Simultaneously, we have not stated significant interactions with other microelements, thus we can conclude about their lack of important interactions on enzymatic activity.
Heavy metal accumulation, physiological status and resistance against Zn and Pb were compared in lichens occurring in metalpolluted (Silesian Upland, S. Poland) and unpolluted (forest complex, NE Poland) areas. Sandy soil near Zn/Pb ore mine had significantly lower (5–35-times) metal content than dolomite-containing ground of mine tailing dump in polluted area (S. Poland). Metal contents in native lichens sampled from those sites followed the same pattern. Total Zn, Pb, Cd and Cu contents in native lichens recorded in two sites of Zn/Pb mining area varied broadly and were considerably higher than in lichens from a control unpolluted site. Independently of the sampling site, epiphytic lichens of higher surface to biovolume ratio (i.e. Candelariella Mull. Arg., Lepraria Ach.) accumulated much more metals (6.05 – 9.57 mg g–1 DW) than lichens of a lower ratio (e.g. 0.25 mg g–1DW in Peltigera didactyla (With.) J.R.Laundon or 0.29 mg g–1 DW in some Cladonia Hill ex Browne). In general, the studied lichens accumulated metals in the following order: Zn>Pb>Cd≥Cu, that was in agreement with the metal content in soils. However, Cladonia furcata (Huds.) Schrad. From tailing dump contained 2-fold more Pb than Zn. Internal Zn and Pb contents (non-exchangeable fraction) in the studied lichens ranged broadly from 17 to 90% of the total metal content. Phaeophytinisation quotients (PhQ), total chlorophyll contents and chl a/b ratios in the native lichens from polluted sites ranged 0.84 – 1.44; 0.514 – 4.858 mg g–1 DW and 2.09 – 5.56, respectively. Experimental exposure of selected species (Hypogymnia physodes (L.) Nyl., Hypocenomyce scalaris (Ach.) Choisy, Lepraria elobata Tonsberg, L. incana (L.) Ach., sampled from the both polluted and unpolluted sites, to high doses of Zn2+ and Pb2+ (0.36 or 0.72 mmol g–1 DW) revealed higher resistance of lichens from the Zn/Pb-polluted sites than con-specific lichens from the unpolluted one. The resistance of epiphytic species from the polluted area to high doses of Zn2+ and Pb2+ increased as follows: H. physodes
Carotenoids (neoxanthin, violaxanthin, lutein and β-carotene) present in plant cell play a role in photoprotection. Industrial pollution causes oxidative stress in plants, while carotenoids react with free radicals and dissipate the excess of excitation energy. In this way carotenoids prevent the negative influence of free radicals on metabolism and can even restore some of the damages. This is confirmed by results of our analysis of the level of xanthophylls in 16 and 17-year-old trees of Scots pine (Pinus sylvestris L.). Developed from seed collected in north-eastern, the trees grow in a relatively unpolluted control site, and in a polluted site located 2 km for away from the Phosphorus Fertilizer Works. In the polluted site the environment is contaminated with SO2, NOx and F, Al, F, Pb, Cu. The needles analysed in this study were visually undamaged. Material was collected in experimental plot from 6 trees in October'98 and April'99, between 12.00 and 13.00 hours, at full sunlight. The pigments were analysed quantitatively and qualitatively by thin-layer chromatography. The paper presents results of content and distribution of neoxanthin, violaxanthin, lutein and β-carotene of Scots pine needles from a healthy control tree and a stressed tree. Marked differences in pigment levels depended on the stage of needle development and level of pollution.
Określono status mikoryzowy Miscanthus х gigantem Greef et Deuter - azjatyckiego gatunku wieloletniej trawy uprawianej obecnie do celów energetycznych. Miskanta uprawiano na terenie zanieczyszczonym Cd, Zn i Pb w Bytomiu oraz na poletku kontrolnym w Katowicach. Na wszystkich poletkach badawczych w korzeniach miskanta stwierdzono obecność struktur typowych dla mikoryzy arbuskularnej. Nie zaobserwowano różnic w poziomie kolonizacji mikoryzowej pomiędzy poletkiem kontrolnym a poletkami zanieczyszczonymi metalami. Kumulacja Cd, Zn i Pb w pędach i korzeniach miskanta rosła wraz z ich całkowitą zawartością w glebie. W większości przypadków wyższe zawartości metali odnotowywano w korzeniach niż w pędach.
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