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Recently, the importance of silicon (Si) has been demonstrated for many plant physiological processes. The recognized function of Si is to help plants to overcome multiple biotic and abiotic stresses, e.g. disease or pests, low temperature, water deficit, salinity or heavy metals. Silicon advantageously impact on plant development and may improve the quality of ornamental plants. Hosta is an ornamental perennial, that flowers can serve as a source of nectar and pollen for floral visitors. The effect of artificial silicon (Si) supply on flowering, nectar production and pollen traits in Hosta Tratt. ‘Krossa Regal’ was studied. A foliar spraying of 0, 120, 180 and 240 mg Si·dm–3 was applied with water solutions of Actisil Hydro Plus, containing silicon in the form of H4SiO4. Silicon supply in concentration of 180 and 240 mg Si·dm-3 affected the morphometric traits of the flower (perianth width and floral tube length), but not the number of flowers produced per inflorescence. The silicon supply in concentration of 180 and 240 mg Si·dm-3 resulted in the increase of nectar production and in sugars excess in floral nectar. Treatments in concentration of 180 and 240 mg Si·dm-3 positively influence both pollen production and pollen viability. Presumably, most of these results can be explained through the effect of the Si on metabolism enhancement, e.g. by water balance regulation and increase in photosynthetic efficiency.
This study on blooming biology, nectar secretion, pollen production and insect visitation of Aquilegia vulgaris L. was carried out in 2009 and 2011 in Lublin. The peak of flower opening during the day was between 5.00 and 7.00 (GMT +2). The flowers are protandrous with the female phase beginning approx. on the 3rd day of anthesis. The dynamics of nectar secretion and pollen shed from anthers (progressing from the central part of the androecium outwards) support the reproductive system. The amount of nectar accumulated in the spurs increased from the bud stage and was the highest in the phase with approx. ¾ of dehisced anthers, usually on the 3rd day of flower life. Then, towards the end of anthesis, the amount of secreted and accumulated nectar decreased. The number of anthers developed per flower varied from 41 to 61 (mean = 49.1). The mass of pollen per 100 anthers averaged 6.7 mg. Pollen production per flower (mean = 3.28 mg) slightly varied between years and was mainly correlated with the number of developed anthers. Estimated pollen yield was 1.69 g per m2 and sugar yield 1.22 g per m2. Species from the genus Bombus were the main flower visitors, with B. terrestris being the most frequent forager.
The studies concerned the variability in nectar and pollen production in flowers of 75 double-low lines of white mustard (Sinapis alba L.) and a reference cultivar ‘Nakielska’. Also, rates of insect visitation to flowers in 19 lines were investigated. Significant genotypic differences were found in nectar amount, sugar concentration in nectar and total nectar sugar amount. The average nectar amount/10 flowers varied from 2.3 to 24.4 mg, with a mean 14.7 mg for ‘Nakielska’. Nectar concentration for most lines exceeded 20%. The majority (52%) of the lines tested produced more than 2 mg of sugars/10 flowers in nectar. The average amounts of pollen varied from 1.2 to 12.0 mg/10 flowers, with the mean 8.5 mg for ‘Nakielska’. No clear genotypic effect on the amount of pollen/10 flowers was demonstrated. The lines did not differed significantly in their attractiveness to flower visitors. The main foragers were honey bees (93% of all visiting insects). It is concluded that the double-low lines of S. alba are comparable to conventional cultivars in their potential for nectar flow but perform poorer as pollen producers. The studies concerned the variability in nectar and pollen production in flowers of 75 double-low lines of white mustard (Sinapis alba L.) and a reference cultivar ‘Nakielska’. Also, rates of insect visitation to flowers in 19 lines were investigated. Significant genotypic differences were found in nectar amount, sugar concentration in nectar and total nectar sugar amount. The average nectar amount/10 flowers varied from 2.3 to 24.4 mg, with a mean 14.7 mg for ‘Nakielska’. Nectar concentration for most lines exceeded 20%. The majority (52%) of the lines tested produced more than 2 mg of sugars/10 flowers in nectar. The average amounts of pollen varied from 1.2 to 12.0 mg/10 flowers, with the mean 8.5 mg for ‘Nakielska’. No clear genotypic effect on the amount of pollen/10 flowers was demonstrated. The lines did not differed significantly in their attractiveness to flower visitors. The main foragers were honey bees (93% of all visiting insects). It is concluded that the double-low lines of S. alba are comparable to conventional cultivars in their potential for nectar flow but perform poorer as pollen producers.
Wild growing rose species are of great importance as a source of pollen for insects. Oil extracted from the petals of various Rosa species is used in perfumery, cosmetic industry, and therapeutics. In our study, we compared the flowering duration and flower lifespan, the number of stamens and pistils, the mass and size of pollen grains as well as the anatomical features of the petals of four Rosa species: R. canina, R. ×damascena, R. gallica, and R. rugosa. Moreover, we examined the pollen loads collected by bumblebees foraging on rose flowers in order to determine the attractiveness of pollen of this genus to insects. We showed the flower lifespan to vary (3.5–8 days) in the roses studied and revealed high variation in the number of stamens (82–260) and pistils (17–65) as well as in the mass of pollen produced. The flowers of R. rugosa produced the highest amount of pollen (26.7 mg per flower), while the flowers of R. canina the least (3.3 mg per flower), which is associated with differences in the number of stamens developed in the flowers between these species. The largest pollen grains were found in R. ×damascena and R. gallica. We demonstrated that R. ×damascena produces the thickest petals and that scent-emitting papillae found on the adaxial surface of the petals differ in size and shape in the rose species investigated.
Flowering phenology, diurnal dynamics of blooming, insect visitation and pollen production in Aconitum lycoctonum L. and Aconitum carmichaelii Debeaux were investigated in 2012–2013 in the Lublin area, SE Poland. Flowering of A. lycoctonum occurred in June/July, whereas A. carmichaelii bloomed in September/October. Both Aconitum species differed in terms of the diurnal pattern of flowering. The flowers of A. lycoctonum started opening at 5.00, whereas those of A. carmichaelii started blooming at 8.00 (GMT+2h). The species differed in the number of anthers per flower, the size of anthers, and the mass of pollen produced in anthers. As a result, the flowers of A. lycoctonum produced less pollen (mean = 1.0 mg per 10 flowers) than the flowers of A. carmichaelii (mean = 8.2 mg per 10 flowers). The estimated pollen yield was 0.2 g per m2 for A. lycoctonum and 1.6 g per m2 for A. carmichaelii. The flowers of both Aconitum species were foraged exclusively by bumblebees with the predominance of the long-tongued Bombus hortorum. Nectar was a more attractive floral reward than pollen. The propagation of Aconitum lycoctonum and A. carmichaelii in ornamental gardens may support the conservation of bumblebees whose populations are steadily declining.
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In the study, structural features of fl owers of the following allergenic plant species were analysed: Betula verrucosa, Secale cereale, Rumex acetosella, Plantago major and Artemisia vulgaris. Pollen production was established by calculating the number of pollen grains produced by the stamen, fl ower and infl orescence. The dates of occurrence and pollen grains concentration in the air of Lublin were determined. A positive correlation was found between the length of anthers and the number of pollen grains produced. The largest number of pollen grains per anther is produced by Secale cereale (22 360), whereas the smallest one by Plantago major (5 870). The other species produced intermediate numbers of pollen grains in the anther: Betula verrucosa – 11 160, Rumex acetosella – 10 850, Artemisia vulgaris – 9 580. The birch pollen season in Lublin lasts about a month, and pollen of this taxon reaches the highest airborne concentrations among the studied taxa. Low values of pollen concentrations are characteristic for rye and plantain, whereas slightly higher values are recorded for sorrel pollen. Mugwort pollen reaches high concentrations which are noted at the beginning of August.
Although aerobiological data are frequently used as a fl owering sign in phenological research, airborne pollen counts are infl uenced by a number of factors affecting pollen curves. A study was made about the reproductive biology of birch and environmental factors infl uencing its pollen release and transport, in order to achieve a reliable interpretation of Betula pollen curves. Aerobiological data were recorded in 2002 and 2003 at two sites in NW Spain and phenological observations were carried out on 20 trees from four Betula populations (three Betula alba L. and one B. pendula Roth.). Pollen production was calculated for six Betula alba trees. Chilling and heat requirements for triggering development were calculated. Due to differences in the geographical location, budbreak and fl owering started fi rst in Betula pendula. The fl owering period lasted from 8-13 days. Reduced pollen output per anther and catkin in individual trees in 2003 prompted a marked decline in overall pollen production. Major differences observed in birch pollen curves were attributed to the infl uence both of weather conditions and pollen transport from areas where the fl owering occurs at a different time. Heat requirements calculated using phenological and aerobiological data were similar when the peak pollen- count date was used.
Knowledge of the flowering phase and plant pollination ecology is very important for understanding the life history of long-lived perennials. In the case of rare species, the information may have implications for conservation practices. Our studies on flower morphology and blooming biology of the vulnerable plant Senecio macrophyllus M. BIEB. were conducted in situ (flowering, activity of insect visitors) in the largest population in SE Poland and in laboratory (light and scanning electron microscopy). The disc florets open diurnally with most intensive anthesis in the early afternoon and attract insect visitors with nectar and pollen. In highly protandrous disc florets, pollen viability decreases in time, whereas stigma receptivity increases. The upper part of the pistil forms a brush-like pollen presenter, covered with unicellular trichomes with chromoplasts. Pollen presentation lasts 4–11 hours per floret, and 8 days in a single head, the main function of which is to extend the availability of male gamets for reproduction. The number of florets per head, the head size, and the number of pollen grains produced per anther, and the pollen grain viability differed significantly between microsites. The finding indicates that, apart from the biotic factors, abiotic conditions may considerably influence generative reproduction of the species. In the study area, the principal visitors of S. macrophyllus flowers were Hymenoptera, with predominance of Apis mellifera (53.4% of visits). The remarkable share of butterflies (13.9%) recognized as the long-distance moving insects may improve the genetic variability of individuals within a fragmented population. A more detailed study is necessary to explain the role of insect visitors in effective pollination and in gene transfer between patches. The assessment of optimal conditions for the generative reproduction is fundamental for the in situ conservation of this rare species.
This paper presents the results of an analysis of pollen season patterns for taxa which show the strongest allergenic activity (alder, birch, grasses, and mugwort) in 2008 in the air over Wrocław and Olszanica. The study was carried out using the volumetric method (Burkard trap). The results show variation in pollen seasons between the analyzed localities. An attempt was made to find out in which of the sites in question – the urban site or the rural one – there was a greater risk of allergens of the selected plants. The results of the present study show that the alder, birch and grass pollen seasons in 2008 started and ended earlier in Wrocław, and maximum pollen concentrations were definitely lower. But the mugwort pollen season started earlier and ended much later in Olszanica, while maximum pollen concentration of this taxon was more than twice lower than in Wrocław. In 2008 in the investigated localities, the highest pollen concentrations of the plants in question occurred in the following months: alder in February, birch in April, grasses in June, while mugwort in August. In 2008 alder and birch pollen allergen risk was comparable in the investigated urban and rural environment. However, grass and mugwort pollen allergens posed a significantly greater threat in the rural environment than in Wrocław.
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