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1

Immunolocalization of lipoxygenase in the anther of Gagea lutea [L.] Ker.-Gaw.

100%
Szczuka E., Skorzynska-Polit E., Pawlikowska-Pawlega B., Sobieska J., Gawron A.
Acta Biologica Cracoviensia. Series Botanica
|
2006
|
tom 48
|
nr 1
Immunogold labelling revealed the presence of lipoxygenase (LOX) in different parts and types of anther cells of Gagea lutea. LOX was found in the cytoplasm and close to ER elements in epidermal and endothecial cells, and close to the cell walls of the latter. The positive immunoreaction to LOX was less intense in the middle layers and the loculus of the anther, where single immunogold particles were concentrated at the cell walls of these layers and in the protoplast masses, in vacuoles, close to mitochondria, inside plastids, and in the liquid of the anther cavity. LOX occurred in the cytoplasm and around ER elements of pollen grains as well as in the exine layer, particularly in contact regions between the outer and inner exine layers. The correlations between LOX localization in different anther cells and the functioning of particular anther parts are discussed.
2

Tapetal plastids in Ornithogalum virens: from meristematic stage to pollen coat

84%
Lesniewska J., Charzynska M.
Acta Biologica Cracoviensia. Series Botanica
|
2000
|
tom 42
|
nr 2
We examined the ultrastructure of plastids during the development and degradation (programmed cell death) of the secretory tapetum in Ornithogalum virens, a species with a pollen coat. Plastids present in meristematic tapetum cells differentiate into amyloplasts and function as amyloplasts during the period of tapetal activity. They undergo two cycles of starch synthesis and hydrolysis before entering the degradation phase in which their structure disintegrates and plastoglobules form, preceding pollen coat formation. We showed that during these processes the plastids are nonrandomly distributed in tapetal cells and that the spatial relation between the plastids, cell nuclei and ER depends on the stage of tapetum and pollen development. We also showed that polar distribution of the plastids and polar localization of starch hydrolysis activity characterized the phase of intensive tapetal secretion.
3

Pollen morphology and two-dimensional patterns of pollen coat and protoplast proteins in Aegilops kotschyi x Secale cereale amphiploids

84%
Kalinowski A., Klimko M., Wojciechowska B.
Acta Biologica Cracoviensia. Series Botanica
|
2005
|
tom 47
|
nr 2
Amphiploid pollen of Aegilops kotschyi x Secale cereale was compared to the parental form by SEM, and comparatively measured using light microscopy. Pollen grains of amphiploids were larger and more variable in total and pore diameter than the parents. Amphiploid pollen was prolate, subprolate and prolate-spheroidal in shape. The exine of Ae. kotschyi AK-2 and AK-3 had a delicate verrucate surface, whereas Secale cereale S14 exine had a verrucate surface. Amphiploid pollen grain surfaces were more or less similar to those of the parents: delicate verrucate, verrucate and well verrucate. The sculpture of parental and amphiploid pollen grains showed conspicuous granulation. All amphiploids produced pollen with one pore with an operculum, surrounded by a well-defined annulus. Sporophytically produced peptides from the pollen coat and gametophytically produced peptides from the protoplast were analyzed separately by two-dimensional gel electrophoresis. Two accessions ofAe. kotschyi (AK-2, AK-3) showed differences in the 2-D patterns of peptides from both the pollen coat and the protoplast. The majority of pollen coat and protoplast peptides of the parents were detected in the amphiploids, but a number of parental peptides were absent. All the amphiploids possessed peptides in their pollen coat and protoplast not detected in the corresponding pollen fractions of the parents. No relation between colchicine and in vitro amphiploid production and its 2-D patterns was observed. The results of pollen morphology and pollen protein analysis are convergent.
4

Effects of interaction between pollen coat eluates and pistil at the molecular level in self-compatible and self-incompatible plants of Lolium multiflorum Lam.

84%
Kalinowski A., Radlowski M., Bocian A.
Journal of Applied Genetics
|
2006
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tom 47
|
nr 4
Two-dimensional electrophoresis (2-DE) of soluble proteins and enzymes was performed and specific activities of 5 enzymes (esterase, pectinesterase, acid phosphatase, protease and diaphorase) were determined in stigmas of Lolium multiflorum (Italian ryegrass) treated with self or foreign pollen coat eluates (pc). Also, a low-molecular-weight fraction of the treated self-compatible (SC) and self-incompatible (SI) stigmas was analyzed by high-pressure liquid chromatography (HPLC). The treatment of stigmas with foreign pollen induced the loss of 42% of the control sample proteins in SC plants but only of 5.5% in SI plants. In contrast, the treatment of stigmas with foreign pollen induced the loss of 15% proteins in SC plants and of 29% in SI plants. Specific activities of esterase, pectinesterase and diaphorase were higher in SC than in SI stigmas. The 2-DE enzyme patterns indicated qualitative relationships between the presence of some isoforms of acid phosphatase or protease and the treatment with self or foreign pc in SC and SI stigmas. No changes were observed in HPLC profiles of the low-molecular-weight fraction from SC and SI stigmas treated or not with pc. The presented results revealed different reactions of SC and SI stigmas to the treatment with self or foreign pc. Further investigations may explain if any of the observed reactions represent specific reorientations in the style, facilitating cross- or self-pollination.
5

Tapetal plastids in Ornithogalum virens: from meristematic stage to pollen coat

67%
Lesniewska J., Charzynska M.
Acta Biologica Cracoviensia. Series Botanica. Supplement
|
1999
|
tom 41
|
nr 1
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