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Diagnostyka molekularna Pneumocystis carinii wobec nowych danych dotyczacych biologii i epidemiologii

100%

Golab E.

Wiadomości Parazytologiczne

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1999

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tom 45

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nr 3

405-409

A new data from investigations on Pneumocystis carinii were presented as well as an application of molecular techniques to the diagnosis of P. carinii pneumonia.

2

Konferencja nt. pneumocystodozy

100%

Pawlowski Z.

Wiadomości Parazytologiczne

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1990

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tom 36

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nr 5-6

3

Analiza przebiegu doswiadczalnej pneumocystozy u szczurow za pomoca wybranych metod serologicznych

84%

Sobolewska A., Bitkowska E., Golab E.

Wiadomości Parazytologiczne

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2001

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tom 47

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nr 4

717-721

Since serological methods used in the diagnosis of pneumocystosis may be helpful to a various extent, depending on the stage of a diagnosed infection, it was decided to evaluate the usefulness of some the methods in the course of infection. Wistar rats were used in the experiments. The animals were administered hydrocortisone infections for 12 weeks to induce immunosuppression and activate naturally occurring asymptomatic infections with Pneumocystis carinii. Blood samples and specimes of lung tissues were collected, then they were examined for the presence of specific IgG and IgM antibodies, circulating antigen of Pneumocystsis carinii and circulating immune complexes using immunoenzymatic assays. The results of the above experiments indicated, that in an early stage of infection, the examinations of serum samples for circulating immune complexes were helpful, particularly for these with IgM antibodies.

4

Postępy w rozpoznawaniu pneumocystodozy

84%

Mojon M.

Wiadomości Parazytologiczne

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1990

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tom 36

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nr 5-6

5

Pneumocystis carinii: nowe spojrzenie na taksonomie. Epidemiologia i patogeneza pneumocystozy

84%

Pietrzyk A.

Postępy Mikrobiologii

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1998

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tom 37

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nr 2

217-231

6

NESTED PCR w diagnostyce przypadkow pneumocystozowego zapalenia pluc [PCP] u pacjentow z Aids

67%

Golab E., Sobolewska A., Bitkowska E., Bednarska A., Berak H., Dzbenski T. H., Horban A.

Wiadomości Parazytologiczne

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2001

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tom 47

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nr 3

521-525

The studies were undertaken to evaluate the usefulness of a nested PCR assay in the diagnosis of Pneumocystis carinii pneumonia (PCP) in AIDS patients. To achieve the end, 51 excretions samples from the respiratory traci were collected from HIV-infected patients with respiratory symptoms, and examined for the presence of specific DNA. A portion of the mitochondrial large-subunit rRNA gene of Pneumocystis carinii was amplified with outer primers pair pAZ 102E, pAZ 102H and internal primers pAZ 102X, pAZ 102Y. Positive nested PCR results were obtained with 36 out of 51 examined samples. Some 52.8% of the positive results were obtained with samples collected from patients without clinical diagnosis of PCP. It was concluded, that the nested PCR method, being too sensitive, is not suitable for the routine diagnosis of PCP in AIDS patients.

Ograniczanie wyników

5 Wiadomości Parazytologiczne

1 Postępy Mikrobiologii

3 Golab E.

2 Bitkowska E.

2 Sobolewska A.

1 Bednarska A.

1 Berak H.

1 Dzbenski T. H.

1 Horban A.

1 Mojon M.

1 Pawlowski Z.

1 Pietrzyk A.

2 2001

1 1999

1 1998

2 1990

Diagnostyka molekularna Pneumocystis carinii wobec nowych danych dotyczacych biologii i epidemiologii

Konferencja nt. pneumocystodozy

Analiza przebiegu doswiadczalnej pneumocystozy u szczurow za pomoca wybranych metod serologicznych

Postępy w rozpoznawaniu pneumocystodozy

Pneumocystis carinii: nowe spojrzenie na taksonomie. Epidemiologia i patogeneza pneumocystozy

NESTED PCR w diagnostyce przypadkow pneumocystozowego zapalenia pluc [PCP] u pacjentow z Aids