Wyniki wyszukiwania

1

Effect of cytokinins on in vitro morphogenesis and ploidy of pepper Capsicum annuum L.

100%

Tomaszewska-Sowa M., Drozdowska L., Szota M.

Electronic Journal of Polish Agricultural Universities. Series Agronomy

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2002

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tom 05

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nr 1

The study-compared effect of cytokinins on morphogenesis and ploidy of plants regenerated in vitro from explants of Stanola F1, ATZ and ATM pepper seeds. The aim of defining the morphogenetic potential of the studied genotypes, half-seed explants consisting the proximal part of the hypocotyl and radicle were put onto the MS medium containing BAP (5.0 mg.dm-3), 2iP (2.5 mg.dm-3), ZEA (2.5 mg.dm-3), TDZ (1.5 mg.dm-3), while MS medium without cytokinins constituted the control. After the initiation period, explants were transferred onto the medium without cytokinins. The effect of the growth regulators to the morphogenetic response of explants was estimated based on the number of explants on which adventitious buds and shoots were developing. The cytokinins applied did not show a significant effect on the development of adventitious buds on pepper explants.

2

Cell cycle and apoptosis in tumours of oral cavity estimated by image cytometry

100%

Adamska K., Filipiak K., Lewandowski L., Osmola K., Warchol W.

Folia Histochemica et Cytobiologica

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2000

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tom 38

|

nr 1

3

Argyrophilic nucleolar organizer regions, bromodeoxyuridine labelling index and DNA ploidy in squamous cell carcinoma of the uterine cervix

100%

Gasinska A., Zabaglo L., Cichocka A., Pudelek J., Urbanski K., Skolyszewski J.

Folia Histochemica et Cytobiologica

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1996

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tom 34

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nr 2

4

Araceae embryos as tools for ploidy manipulation and somatic fusion

84%

Eeckhaut T., Werbrouck S., Wielowska J., van Huylenbroeck J.

Acta Biologica Cracoviensia. Series Botanica. Supplement

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2005

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tom 47

|

nr 1

5

Improvements of doubled haploid production protocol for white cabbage (Brassica oleracea var. capitata L.)

84%

Rudolf Pilih, K., Potokar U. K., Bohanec B.

Folia Horticulturae

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2018

|

tom 30

|

nr 1

6

Obtaining carrot [Daucus carota L.] plants in isolated microspore cultures

84%

Gorecka K., Kowalska U., Krzyzanowska D., Kiszczak W.

Journal of Applied Genetics

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2010

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tom 51

|

nr 2

141-147

Microspores were cultured on the modified B₅ liquid medium containing 2.4D (0.1 mg L⁻¹), NAA (0.1 mg L ⁻¹), L-glutamine (500 mg L⁻¹), L-serine (100 mg L⁻¹), and sucrose (100 g L⁻¹). The developmental stages of microspores and divisions were observed. Initially, the formation of binuclear and multicellular structures was noticed. Plants regenerated in the cultures in which the tetrad stage of microsporogenesis had predominated. Embryoids were still forming 24 weeks after the cultures were set up. Six weeks after the transfer of androgenetic embryos onto the B₅ regeneration medium, they were converted into complete plants. Out of 90 androgenetic plants planted in a growth chamber, 42 plants adapted to the new conditions. All of those plants proved to be diploids in cytometric analysis.

7

Somatic embryogenesis of the tree fern Cyathea delgadii: ploidy stability of embryo-derived sporophytes

84%

Tomiczak K., Pozoga M., Mikula A., Sliwinska E., Rybczynski J. J.

Acta Biologica Cracoviensia. Series Botanica. Supplement

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2014

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tom 56

|

nr 1

8

Tendency towards facultative apomixis in breeding lines of sugar beet (Beta vulgaris L., Chenopodiaceae)

84%

Szkutnik T.

Acta Biologica Cracoviensia. Series Botanica. Supplement

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2005

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tom 47

|

nr 1

9

DNA ploidy and morphology of colon tumors in the adenoma–carcinoma sequence

84%

Staarmann J., Kotb W. F. A., Petersen I.

Folia Histochemica et Cytobiologica

|

2015

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tom 53

|

nr 1

10

Cytological assessment of carrot plants obtained in anther culture

84%

Kowalska U., Rybaczek D., Krzyzanowska D., Kiszczak W., Gorecka K.

Acta Biologica Cracoviensia. Series Botanica

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2008

|

tom 50

|

nr 2

7-11

Anthers of Feria F1 and Narbonne F1 carrot cultivars were cultured in vitro to induce androgenic embryos. To confirm the microspore origin of developed embryos, chromosome counts of root tip meristematic cells were made for each carrot plant obtained in anther culture. Using phase contrast technique and fluorescence microscopy, cytological changes of microspores during culture leading to proembryo formation were documented in the first days after anther placement on the induction medium. More than 90% of the carrot plants obtained in anther cultures had no haploid chromosomes.

11

Characteristics of the interspecific somatic hybrids Solanum pinnatisectum [plus] S. tuberosum H-8105

84%

Szczerbakowa A., Boltowicz D., Lebecka R., Radomski P., Wielgat B.

Acta Physiologiae Plantarum

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2005

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tom 27

|

nr 3A

The morphological, cyto logical and molecular analyses of the plants regenerated after PEG-induced fusion between mesophyll protoplasts from the dihaploid potato clone H-8105 and the wild tuberous disease-resistant species S.pinnatisectum, were performed. A single fusion experiment yielded 313 calli, although only two calli produced shoots. From the rooted shoots, two stable clones (PT-01-1 and PT-01-2) exhibiting different vigor and habitat, were developed. The plants of PT-01-1 clone grew slowly in vitro, produced tubers after transfer to soil but did not set flowers. In contrast, the plants of the vigorous clone PT-01-2 produced both tubers and flowers after transfer to soil. The flower and tuber morphology of PT-01-1 and PT-01-2 regenerants was intermediate in comparison to the parental species. Cyto logical analysis revealed that the PT-01-1 clone was pentaploid and the PT-01-2 clone was tetraploid. The molecular (RAPD) analysis confirmed hybridity of both clones. The preliminary tests on late blight resistance of the hybrids showed no differences with a potato parent.

12

Characteristics of diploid and triploid kin-tadpoles of Polish water frogs [Rana esculenta complex]

84%

Berger L., Roguski H.

Biological Letters

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2002

|

tom 39

|

nr 1-2

13

Identification of Cobitis from the Slovakian part of the Tisza Basin

84%

Lusk S., Kosco J., Halacka K., Luskova V., Flajshans M.

Folia Biologica

|

2003

|

tom 51

|

nr Suppl.

14

Regeneration of diploid and tetraploid plants of Arabidopsis thaliana via callus

84%

Fras A., Maluszynska J.

Acta Biologica Cracoviensia. Series Botanica

|

2003

|

tom 45

|

nr 2

Different explants from diploid and tetraploid plants of Arabidopsis thaliana ecotype Wilna were cultured in vitro on the same medium in the same conditions. The mode and capacity for regeneration and the ploidy level of regenerants were examined in eight callus lines. The explant responses to in vitro conditions depended on the ploidy level of donor plants and on the type of explant. Callus derived from leaves of tetraploid plants showed the highest ability to regenerate. Histological investigation showed that plants regenerated mostly through organogenesis and occasionally through embryogenesis. Somatic embryos were observed more frequently in callus derived from tetraploid plants. Regenerated plants were diploids, triploids and tetraploids. The majority of regenerated plants from callus of diploid origin were diploid, but diploids were also observed quite frequently among regenerants from callus of tetraploid origin.

15

Organogenesis in endosperm of Actinidia deliciosa cv. Hayward cultured in vitro

84%

Goralski G., Popielarska M., Slesak H., Siwinska D., Batycka M.

Acta Biologica Cracoviensia. Series Botanica

|

2005

|

tom 47

|

nr 2

An efficient procedure for shoot regeneration was obtained by endosperm culture in Actinidia deliciosa cv. Hayward. Mature endosperm cultured on MS medium supplemented with 2 mg/l 2,4-D and 5 mg/l kinetin developed callus with 80% efficiency. Callus was transferred on MS medium containing different plant growth regulators (2,4-D, TDZ, IAA, NAA, BAP, kinetin, 2iP) for regeneration. There were significant differences in regeneration response between medium supplemented with TDZ and medium with other hormones. Only medium containing TDZ stimulated shoot induction. The highest efficiency of shoot regeneration (avg. 6.2 shoots per culture) was on medium supplemented with 0.5 mg/l TDZ. The ploidy of callus and organs formed in endosperm culture was examined by flow cytometry. The results, peaks corresponding to 3C DNA amounts, confirmed the endospermal origin of callus, roots and shoots. Aneuploid and polyploid cells were found in endosperm-derived callus and regenerated organs.

16

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Karyological characterization of sugar beet gynogenetic lines cultured in vitro

84%

Svirshchevskaya A., Dolezel J.

Journal of Applied Genetics

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2001

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tom 42

|

nr 1

17

The use of androgenesis in vitro to obtain homozygous lines of vegetable plants

84%

Gorecka K., Krzyzanowska D.

Vegetable Crops Research Bulletin

|

2001

|

tom 54

|

nr 1

18

Comparative studies of nuclear DNA content in benign and malignant thyroid lesions

84%

Konarska L., Skierski J., Ellert A., Steinbrich J., Wozniak A., Kalczak M.

Acta Biochimica Polonica

|

2001

|

tom 48

|

nr 3

Currently available data suggest that DNA aneuploidy is associated with aggressive behavior of and unfavorable prognosis in several malignant human tumors as compared with diploid malignancies. However, the diagnostic and prognostic importance of flow cytometric DNA measurements in the case of thyroid neoplasms remains controversial. Therefore, the aim of our study was to evaluate utility of DNA index (DI) and proliferative index (PI) in distinguishing benign from malignant thyroid lesions taking into account the possible influence of intra-tumor heterogeneity and tissue preparation mode on DNA flow-cytometry measurements. A retrospective study was performed on 71 paraffin-embedded specimens from 57 patients with benign and malignant thyroid pathologies: 13 colloid goitres, 12 parenchymatous goitres, 19 adenomas and 13 carcinomas. In 14 of 57 cases two separate specimens taken from different areas of the same lesion were analysed and DNA parameters were compared. Additionally, flow cytometry DNA analysis was parallelly performed on 3 adjacent but differently processed tissue sections (fresh, formalin-fixed and paraffin-embedded) taken from each of 26 surgically excised thyroid lesions. DNA content was also analysed in both fresh and formalin-fixed twin specimens of normal pig thyroid glands (N = 6). We demonstrated that all tumors diagnosed as thyroid carcinomas were associated with abnormal nuclear DNA content although aneuploidy was not found specific to malignant thyroid tumors. Aneuploid samples of benign thyroid lesions exhibited higher proliferative activity, expressed as mean PI values, than diploid ones. In carcinomas the mean PI values were significantly higher than in benign lesions, independently whether they concerned aneuploid or diploid tissues. Considering intra-tumor heterogeneity, the flow cytometric DNA parameters can be assumed as reproducible despite differences in the mode of tissue fixation and preparation for analysis.

19

Charakterystyka fenotypowa samosiewow rzepaku ozimego [Brassica napus L.] wystepujacych w polnocnych regionach Polski

67%

Liersch A., Poplawska W., Ogrodowczyk M., Bartkowiak-Broda I., Bocianowski J.

Biuletyn Instytutu Hodowli i Aklimatyzacji Roślin

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2008

|

nr 250

249-260

20

Cytometric analyses of sugar beet (Beta vulgaris L.) plants regenerated from unfertilized ovules cultured in vitro

67%

Tomaszewska-Sowa M.

Electronic Journal of Polish Agricultural Universities. Series Agronomy

|

2010

|

tom 13

|

nr 4

Ograniczanie wyników

Effect of cytokinins on in vitro morphogenesis and ploidy of pepper Capsicum annuum L.

Cell cycle and apoptosis in tumours of oral cavity estimated by image cytometry

Argyrophilic nucleolar organizer regions, bromodeoxyuridine labelling index and DNA ploidy in squamous cell carcinoma of the uterine cervix

Araceae embryos as tools for ploidy manipulation and somatic fusion

Improvements of doubled haploid production protocol for white cabbage (Brassica oleracea var. capitata L.)

Obtaining carrot [Daucus carota L.] plants in isolated microspore cultures

Somatic embryogenesis of the tree fern Cyathea delgadii: ploidy stability of embryo-derived sporophytes

Tendency towards facultative apomixis in breeding lines of sugar beet (Beta vulgaris L., Chenopodiaceae)

DNA ploidy and morphology of colon tumors in the adenoma–carcinoma sequence

Cytological assessment of carrot plants obtained in anther culture

Characteristics of the interspecific somatic hybrids Solanum pinnatisectum [plus] S. tuberosum H-8105

Characteristics of diploid and triploid kin-tadpoles of Polish water frogs [Rana esculenta complex]

Identification of Cobitis from the Slovakian part of the Tisza Basin

Regeneration of diploid and tetraploid plants of Arabidopsis thaliana via callus

Organogenesis in endosperm of Actinidia deliciosa cv. Hayward cultured in vitro

Karyological characterization of sugar beet gynogenetic lines cultured in vitro

The use of androgenesis in vitro to obtain homozygous lines of vegetable plants

Comparative studies of nuclear DNA content in benign and malignant thyroid lesions

Charakterystyka fenotypowa samosiewow rzepaku ozimego [Brassica napus L.] wystepujacych w polnocnych regionach Polski

Cytometric analyses of sugar beet (Beta vulgaris L.) plants regenerated from unfertilized ovules cultured in vitro