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Cross-linking of nuclear proteins to DNA by platinum compounds

100%

Walter Z., Wozniak K., Kowalik J.

Cellular and Molecular Biology Letters

1997

tom 02

nr 4

In this work we investigated the lymphocyte nuclear proteins which participated in DNA-protein cross-links induced by three platinum compounds: cis-DDP, trans-DDP and carboplatin. Our studies indicated that many proteins were cross- linked to DNA in the intact cell. Trans-DDP was the most effective cross-linker. The cross-linking process depended on time of the cells incubation with aforementioned compounds. Carboplatin produced the DNA-protein cross-links more slowly and less effectively than cis-DDP. After incubation of the cells with cis-DDP, trans- DDP and carboplatin a similar protein pattern was obtained. In case of all compounds studied 34.5 kDa protein was abundantly represented.

The protective effects of resveratrol against changes in blood platelet thiols induced by platinum compounds

84%

Olas B., Wachowicz B., Bald E., Glowacki R.

Journal of Physiology and Pharmacology

2004

tom 55

nr 2

Cisplatin (cis-diamminedichloroplatinum II, cisPt) is especially useful in the treatment of epithelial malignancies, however, the use of cisplatin is accompanied by several toxicities including haematological toxicity. Contrary to cisplatin, selenium-cisplatin conjugate ((NH3)2Pt(SeO3); Se-Pt) has only a slight toxicity effect on blood platelet function. In the mechanism of platinum compounds action on platelets thiols are involved. The aim of the present studies was to examine in vitro how trans-resveratrol (trans-3,4',5-trihydroxystilbene) acts on the levels of platelet glutathione (GSH) and other thiol-containing compounds and how, as an antioxidant, protecs blood platelets against the oxidative stress caused by platinum compounds (cisPt and Se-Pt). To analyse the level of thiols in human blood platelets treated with platinum compounds and with resveratrol the classical technique HPLC has been used. Blood platelets isolated by differential centrifugation of human blood were incubated (30 min, 37°C) with cisPt or Se-Pt at dose of 10 µg/ml that inhibits platelet function and with resveratrol (25 µg/ml). The obtained results indicate that platinum compounds caused in platelets a decrease of both, reduced glutathione (GSH) and free thiols of cysteine (CSH) and cysteinylglycine (CGSH). The pool of these compounds in unreduced form was increased. Platinum compounds caused the reduction of platelet protein thiols. Resveratrol (after 30 min action) at the concentration of 25 µg/ml partly reduced the platinum compounds induced decrease of platelet thiols, particularly thiols in acid-soluble fraction.

Ograniczanie wyników

1 Cellular and Molecular Biology Letters

1 Journal of Physiology and Pharmacology

1 Bald E.

1 Glowacki R.

1 Kowalik J.

1 Olas B.

1 Wachowicz B.

1 Walter Z.

1 Wozniak K.

1 2004

1 1997

Wyniki wyszukiwania

Cross-linking of nuclear proteins to DNA by platinum compounds

The protective effects of resveratrol against changes in blood platelet thiols induced by platinum compounds