Wyniki wyszukiwania

1

Functions coded by plasmids in chemolithotrophic bacteria

100%

Jagusztyn-Krynicka E. K., Brzescinska-Kujawa A., Wolska K. I.

Acta Microbiologica Polonica

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1990

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tom 39

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nr 1-2

2

Restriction map of Thiobacillus versutus plasmid pTAV1

100%

Jagusztyn-Krynicka E. K., Brzescinska-Kujawa A., Wolska K. I.

Acta Microbiologica Polonica

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1990

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tom 39

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nr 1-2

3

Construction of mobilizable cloning vestors derived from pBGS18 and their application for analysis of replicator region of a pTAV202 mini-derivative of Paracoccus versutus pTAV1 plasmid

100%

Bartosik D., Bialkowska A., Baj J., Wlodarczyk M.

Acta Microbiologica Polonica

|

1997

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tom 46

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nr 4

4

Nonspecific transmission of the NPTII gene from E.coli helper plasmid to a plasmid of Agrobacterium tumefaciens

88%

Lesniewicz K., Grygorowicz W., Bartkowiak S., Augustyniak H.

Acta Biochimica Polonica

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1994

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tom 41

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nr 2

5

Transformation of E.coli with plasmids coding for degradation of aromatic structure of phenols

88%

Labuzek S., Mrozik A., Pajak J., Kasiak J.

Acta Biochimica Polonica

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1994

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tom 41

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nr 2

6

Genomic DNA library of Veillonella parvula H2 in the rumen

75%

Liu G. W., Zhang Z. G., Yang W. Y., Yang L. Y., Wang Z.

Bulletin of the Veterinary Institute in Puławy

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2010

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tom 54

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nr 1

15-17

To construct a genomic library of Veillonella parvula H2, total DNA was extracted, sheared by a Hydroshear machine, and the DNA fragments ligated a into Smal-digested vector pUC18 before being transformed into E. coli DH5a. Colonies were selected on Luria-Bertani (LB) plates containing ampicillin, 5-bromo-4-chloro-3-indoyl-3-galactose (X-gal), and isopropy-β-D-thiogalactoside (IPTG) and proliferated. Recombinant plasmids were analysed for the presence of inserted DNA fragments of 3-4 kb by restriction mapping. The titre of the library was determined to be 10⁵ pfu/mL according to the formula N=ln(l-p)/(l-f). The genomic library consisted of 99% of the genome of Veillonella parvula, demonstrating a successful library construction.

7

Active stable maintenance functions in low copy-number plasmids of Gram-positive bacteria. II. Post-segregational killing systems

75%

Dmowski M., Jagura-Burdzy G.

Polish Journal of Microbiology

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2013

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tom 62

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nr 1

Active support is needed for low copy-number plasmids to be stably maintained in bacterial cells. The mechanisms that fulfill this role are (i) partition systems (PAR) acting to separate plasmid molecules to daughter cells and (ii) toxin-andidote (TA) (post-segregational killing-PSK) systems which arrest cell growth until the plasmid reaches the correct copy-number or kill the cells that have not inherited the plasmid. Our knowledge of toxin-antidote systems comes mainly from studies on Gram-negative bacteria. However, some addiction systems of Gram-positive bacteria have been characterized in detail or recently identified. Altogether, they bring new interesting data on toxin-antidote functioning in bacteria.

8

CRISPR/Cas9 immune system as a tool for genome engineering

75%

Hryhorowicz M., Lipinski D., Zeyland J., Slomski R.

Archivum Immunologiae et Therapiae Experimentalis

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2017

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tom 65

|

nr 3

9

Identification of plasmids in Pseudomonas aeruginosa using agarose gel electrophoresis

75%

Wolska K., Bukowski K.

Polish Journal of Veterinary Sciences

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2001

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tom 04

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nr 2

10

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Cloning of the DNA fragments reflecting the open reading frame I and II of the I-18 C gene of Chironomus tentans. II. Preparation of the intermediate vector - bluescript plasmid for ligation with the inserts

75%

Borowicz B. P.

Journal of Plant Protection Research

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1999

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tom 39

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nr 1

The technology used for the cloning of the open reading frame (ORF) I and II reflecting DNA fragments of the I-18 C gene in regard to the preparation of the intermediate vector i.e. the bluescript for the ligation with the ORF I and II reflecting DNA fragments, is presented. The main steps include the Eco RV digestion of the bluescript plasmid, the phenol / methylene chloride extraction of the plasmid, dephosphorylation of the bluescript plasmid and finally its extraction with the phenol and ethanol precipitation.

11

The minimal genome paradox

75%

Wegrzyn G.

Journal of Applied Genetics

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2001

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tom 42

|

nr 3

12

Cloning and expression of the hEGF gene in Saccharomyces cerevisiae

75%

Topczewska J., Bolewska K.

Acta Biochimica Polonica

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1993

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tom 40

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nr 1

13

Effect of coliphage lambda P gene mutations on the stability of the lambda O protein, the initiator of lambda DNA replication

75%

Pawlowicz A., Wegrzyn G., Taylor K.

Acta Biochimica Polonica

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1993

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tom 40

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nr 1

14

Identification of a new restriction endonuclease R.BcrAI, from Bacillus cremoris

75%

Piekarowicz A., Skowronek K.

Acta Microbiologica Polonica

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1995

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tom 44

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nr 3-4

15

Mechanisms of plasmid stable maintenance with special focus on plasmid addiction systems

75%

Zielenkiewicz U., Ceglowski P.

Acta Biochimica Polonica

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2001

|

tom 48

|

nr 4

The stable inheritance of bacterial plasmids is achieved by a number of different mechanisms. Among them are resolution of plasmid oligomers into monomers, active plasmid partitioning into dividing cells and selective killing of plasmid-free segre- gants. A special focus is given to the last mechanism. It involves a stable toxin and an unstable antidote. The antidotes neutralize their cognate toxins or prevent their synthesis. The different decay rates of the toxins and the antidotes underlie molecular mechanisms of toxin activation in plasmid-free cells. By eliminating of plasmid-free cells from the population of plasmid-bearing ones the toxin-antidote couples therefore act as plasmid addiction systems.

16

UV- and MMS-induced mutagenesis of lambdaO[am]8 phage under nonpermissive conditions for phage DNA replication

75%

Krwawicz J., Czajkowska A., Felczak M., Pietrzykowska I.

Acta Biochimica Polonica

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2003

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tom 50

|

nr 4

Mutagenesis in Escherichia coli, a subject of many years of study is considered to be related to DNA replication. DNA lesions nonrepaired by the error-free nucleotide excision repair (NER), base excision repair (BER) and recombination repair (RR), stop replication at the fork. Reinitiation needs translesion synthesis (TLS) by DNA polymerase V (UmuC), which in the presence of accessory proteins, UmuD', RecA and ssDNA-binding protein (SSB), has an ability to bypass the lesion with high mutagenicity. This enables reinitiation and extension of DNA replication by DNA polymerase III (Pol III). We studied UV- and MMS-induced mutagenesis of λO(am)8 phage in E. coli 594 sup host, unable to replicate the phage DNA, as a possible model for mutagenesis induced in nondividing cells (e.g. somatic cells). We show that in E. coli 594 sup cells UV- and MMS-induced mutagenesis of λO(am)8 phage may occur. This mutagenic process requires both the UmuD' and C proteins, albeit a high level of UmuD' and low level of UmuC seem to be necessary and sufficient. We compared UV-induced mutagenesis of λO(am)8 in nonpermissive (594 sup ) and permissive (C600 supE) conditions for phage DNA replication. It appeared that while the mutagenesis of XO(am)8 in 594 sup+ requires the UmuD' and C proteins, which can not be replaced by other SOS-inducible protein(s), in C600 supE their functions may be replaced by other inducible protein(s), possibly DNA polymerase IV (DinB). Mutations induced under nonpermissive conditions for phage DNA replication are resistant to mismatch repair (MMR), while among those induced under permissive conditions, only about 40% are resistant.

17

Combined effect of strigent or relaxed response, temperature and rom function on the replication of pUC plasmids in Escherichia coli

75%

Herman A., Wegrzyn A., Wegrzyn G.

Acta Biochimica Polonica

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1994

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tom 41

|

nr 2

18

Vectors to express viral genes

75%

Plucienniczak A.

Bulletin of the Veterinary Institute in Puławy

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1998

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tom 42

|

nr 1

19

Plasmid-mediated suppression of the mutational activation of the bgl operon in Shigella sonnei

75%

Kharat A. S., Mahadevan S.

Acta Biochimica Polonica

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1999

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tom 46

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nr 4

SSOR, a clinical isolate of Shigella sonnei which exhibits a Salicin-negative phenotype, is unable to mutate to give rise to Sal+ derivatives although a homolog of the Escherichia coli bgl operon is retained by the strain. This was correlated to the presence of an endogenous plasmid in the strain. A plasmid-cured derivative, AK711, could give rise to Sal+ mutants in two steps. Introduction of the plasmid DNA, extracted from SSOR, into various strains of E.coli and S. sonnei, resulted in ampicillin resistant transformants. Interestingly, the presence of the plasmid suppressed the mutational activation of the bgl operon in the transformants. This was further substantiated by the observation that, transformants that have lost the plasmid regained the ability for mutational activation of the bgl operon. Preliminary characterisation of the plasmid indicated a size of 3.8 kb with an origin of replication resembling that of ColE1 replicons and the bla gene homolog of Tn3. Observations of the mutation frequency at the srl and lac loci in the presence of the plasmid indicate that there is a reduction in the mutation frequency, suggesting an antimutator activity associated with the plasmid.

20

Combined tumor cell-based vaccination and interleukin-12 gene therapy polarizes the tumor microenvironment in mice

63%

Jarosz-Biej M., Smolarczyk R., Cichon T., Kulach N., Czapla J., Matuszczak S., Szala S.

Archivum Immunologiae et Therapiae Experimentalis

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2015

|

tom 63

|

nr 6

Ograniczanie wyników

Functions coded by plasmids in chemolithotrophic bacteria

Restriction map of Thiobacillus versutus plasmid pTAV1

Construction of mobilizable cloning vestors derived from pBGS18 and their application for analysis of replicator region of a pTAV202 mini-derivative of Paracoccus versutus pTAV1 plasmid

Nonspecific transmission of the NPTII gene from E.coli helper plasmid to a plasmid of Agrobacterium tumefaciens

Transformation of E.coli with plasmids coding for degradation of aromatic structure of phenols

Genomic DNA library of Veillonella parvula H2 in the rumen

Active stable maintenance functions in low copy-number plasmids of Gram-positive bacteria. II. Post-segregational killing systems

CRISPR/Cas9 immune system as a tool for genome engineering

Identification of plasmids in Pseudomonas aeruginosa using agarose gel electrophoresis