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The Niepołomice Forest, a large forest complex typical of Central European lowlands, is located near an urban-industrial agglomeration in southern Poland. During the past 20 years, SO₂ emissions from industry decreased from 75,000 t yr⁻¹ to 6000 t yr⁻¹. Concentration of SO₂ in the air, and pH, SO₄⁻² concentration in bulk precipitation were measured during the last 11 years and compared with data from the 1970s. The spatial distribution of concentrations and deposition of S were calculated on the basis of 30 bulk precipitation samples in 1999-2000. Mean annual concentration of SO₂ in the air was 8.25 μg m⁻³ in 2000, that is 2.5 times lower than during the period 1967-1978. At the beginning of the 1990s the concentration of SO₂ exceeded the critical level for plants during 3% of the days over the year, and at the end of the 1990s only on single days. In 1999 and 2000 the yearly mean concentration of S-SO₄⁻² in bulk precipitation in the Niepołomice Forest was 1.11 mg l⁻¹, ranging between 0.99 and 1.27 mg l⁻¹ in the forest complex. The yearly mean sulphur deposition (S-SO₄⁻²) was 8.1 kg ha⁻¹, varying from 7.3 to 8.9 kg ha⁻¹. The present concentrations and deposition of S-SO₄ were a quarter those of 1974-1978. The spatial distribution of S deposition pointed to the influx of S compounds to the Niepołomice Forest with air masses from the west. The yearly mean pH of atmospheric precipitation was 4.64 (1999-2000), and was steady within the forest complex. In the Niepołomice Forest, atmospheric precipitation was more acid recently than in the 1970s.
The research included a static crop-rotation field experiment set up in 1993: sugar beet – winter wheat – faba bean – winter barley + stubble intercrop and covered the effect of three soil cultivation systems: A – ploughing cultivation, B – ploughless cultivation, C – direct sowing on selected chemical properties of soil sampled from three soil profile layers: 0 – 10 cm, 10 – 20 cm and 20 – 30 cm. The ploughless soil cultivation and direct sowing increased the contents of organic C, total N and available K and decreased the content of exchangeable Mg in the upper soil layers. The content of available P and exchangeable Ca did not depend on the factors studied significantly. Giving up ploughing cultivation and taking up direct sowing increased the sum of exchangeable bases and the tendency to decrease soil pHKCl.
The usual range of P in soils is of the order of 500 to 800 mg kg⁻¹; in Hungarian soils it is 200-900 mg kg⁻¹ [3,9]. Total P is usually highest in the upper A horizon and lowest in the lower A and upper B horizons because of recycling by plants. The organic P content (10-60 % of total-P) is the highest in the top layer and decreases with depth in all soils [9].
From the early 60's till the late 80's, in many countries - among them also in Hungary - agricultural production went through a tremendous development. In Hungary there was a 2-3-fold increase in the average yields of winter wheat, and maize - the two main crops - in comparison to those of the 50's. One of the main factors determining these developments was the increasing use of mineral fertilizers. From the turn of the century till the late 50's, nutrient balances in Hungary were strongly negative: 20 - 30 kg/ha/year less N and K₂O, and 10 kg/ha/year less P₂O₅ was given to the fields in the different forms (farmyard manure, mineral fertilizer and by-products, etc.), than was removed by the harvested yields. Nutrient balances of P became positive in the early 60's, while balances of N and K in the early 70's, resp. Then, for 20 years, N balances were positive by 10 - 20 kg/ha/year, while both P₂O₅ and K₂O balances by 30 - 50 kg/ha/year, resp. These long-term positive nutrient balances resulted in the NPK enrichment of our soils, which was also proven by the national soil test series. During the 80's, for example, the amount of yearly applied mineral fertilizer was 230 - 280 kg/ha N + P₂O₅ + K₂O/arable land. In certain regions N leaching could cause environmental damage, while, as a result of the P-Zn antagonism, on the fields poorly supplied with Zn, overfertilization with P resulted in 1 - 2 t/ha/year maize yield losses. From the early 90's, however, when political and ecological changes took place in the country, the free market was introduced, and state subsidies on mineral fertilizer were withdrawn, there was a sharp decrease in mineral fertilizer use: applied N dropped to 1/5th, P and K to 1/20th of the amount used in the 80's. This dramatic decrease resulted in the change of nutrient balances: in 1991 the balance for N was -60, for P₂O₅ -30, and for K₂O -40 kg/ha for the whole country, resp. In 1992 and 1993 the situation was similar, while in 1994 a slight increase in mineral fertilizer use was observed. According to our estimation, mineral fertilization of 150 kg/ha/year N + P₂O₅ + K₂O is sufficient for long-term sustainable plant nutrition in Hungary, if farmyard manure application and the incorporation of byproducts remains on the recent level. During intensive fertilization practice, emphasis was on the quantity, while crop and soil demands for specific or more economic fertilizers were not taken into consideration. For N, lime ammonium nitrate, for P superphosphate, and for K, potash chloride was used on almost the whole area. As a new attempt to find more economic P sources, Algerian rock phosphate was checked in field trials, set up on characteristic acidic soils in different regions of Hungary, as well as other Central European countries. The first two-year results are discussed in the presentation.
Salt stress restricts plant performance by disrupting various physio-biochemical processes like photosynthesis. Plants growing in saline substrates show deficiencies in absorption of some essential elements due to the presence of excessive sodium (Na⁺) in the rhizosphere, which antagonizes beneficial cations and causing toxicity in metabolism. Cyanobacteria (CB; a natural biofertilizer) play a fundamental role in building-up soil fertility, thus increasing plant performance. Glutathione (GSH) is a well-known antioxidant, which contributes to increase salt tolerance in the plant. This work was conducted as a pot experiment (sand culture) in 2017 to study the combined effect of CB, applied as seed inoculation, and GSH, applied as foliar spray, on growth, pods and seed yields, the contents of antioxidants, osmoprotectants, and nutrients, and the antioxidative enzymes activities of soybean (Glycine max L., cv. Giza 111) plants grown under saline conditions. At fourth leaf stage (21 days after sowing; DAS), CB-pretreated seedlings were supplemented with NaCl (150 mM) along with Hoagland′s nutrient solution, and at the same time seedlings were sprayed with 1 mM GSH. Samples were taken at 60 DAS to assess morphological, physio-biochemical and antioxidant defense systems attributes. Results showed that the integrative application of CB and GSH under saline conditions was effective in improving significantly the growth characteristics, yield components, photosynthetic efficiency (pigments contents and chlorophyll fluorescence), membrane stability index, relative water content, contents of soluble sugars, free proline, ascorbic acid, glutathione, α-tocopherol, and protein, and activities of superoxide dismutase, catalase, and guaiacol peroxidase. The contents of macronutrients (N, P, K⁺, and Ca²⁺) were also increased significantly in Glycine max plants compared to the stressed control. In contrast, Na⁺ content and electrolyte leakage were significantly reduced. Our results recommend using the combined CB (as seed inoculation) and GSH (as foliar spray) application for soybean plants to grow well under saline conditions.
The purpose of this study was to design the formula and antioxidant potential of gluten-free oat drinks fortifi ed with bioactive plant components. The paper describes the use of three preparations of plant origin: yellow tea leaf extract, mulberry extract and steviol glycosides from stevia leaves. Analyses of the oat drinks basic composition – including proteins, lipids, carbohydrates, dietary fi ber fractions and antioxidant properties with DPPH, ABTS, ORAC and FRAP assays were conducted. Tea extract with steviosides oat drink showed the highest total phenolic concentration and the highest antioxidant activity followed by stevia and mulberry oat drink, whereas stevia oat drink showed the lowest phenolics level and weakest antioxidant activity. Moreover, the applied plant components showed moderate ability to influence the sensory values of the fortifi edoat drinks. The results demonstrate that tea, stevia and mulberry preparations applied in oat drinks enhanced antioxidant potential and might be a natural source of antioxidant components.
In a 3-year pot experiment, the fertilizing value was investigated of fresh and 3 month composted waste activated sludges, FYM and their mixtures with CaO and brown-coal ash. Higher yields of maize and sunflowers were achieved from objects fertilized with waste activated sludges with the addition of ash than those with the addition of CaO or without any addition. The effectiveness of waste activated sludges from Siedlce and Łuków, applied as either fresh or composted, was similar. Higher yields were achieved from objects fertilized with waste activated sludges than fertilized with FYM. The content of nitrogen was higher in the biomass of maize and sunflower harvested from objects fertilized with only waste activated sludges than those with mixtures of waste activated sludges with CaO and brown-coal ash. The highest value of nitrogen utilization coefficient calculated for the whole period of the experiment was achieved from objects fertilized with waste activated sludges and FYM with the addition of ash, slightly lower from objects on which waste activated sludges and FYM with the addition of ash and CaO were applied, and the lowest from objects on which mixture of FYM and waste activated sludges with the addition of CaO were applied.
Lublin municipal waste water effluent treated in two stages (mechanical and biological) was used in the monoliths filled with loess soil of an undisturbed structure taken from different erosion zones: W - top (grey-brown podzolic soil): D - bottom of the valley (deluvial soil); N - northern slope (brown soil - weakly eroded), S - southern slope (calcareous soil - strongly eroded). Control objects were the monoliths treated with equal amounts of water and nutrients: N, P, K, Mg. In the soils treated with sewage and water and mineral salts, a similar increase in the content of soluble metal forms (Zn, Cu, Mn, Pb, Cd, Ni) was observed.
Greenhouse tomato plants (cv. Cunero F₁) were grown in the NFT units with plastic troughs filled with either rockwool or coconut fiber. The initial concentrations of sulphates in the nutrient solutions were 200, 400 or 600 mg SO₄⁻² dm⁻³. The effect of different sulphate levels and type of medium on the yield, mineral composition and quality of tomato were studied. Regardless of the initial sulphate level or type of medium, the accumulation of sulphates in the nutrient solution was observed in the begining growing period. However, the organic medium (coconut fiber) cumulated more sulphates than mineral one. On the other hand, there was a higher concentration of sulphates in the recycling solution in the units with mineral (rockwool) medium compared to coconut fiber. Regardless of the type of medium the accumulation of sulphates in the root zone had no influence on the total and marketable yields as well as on the level of ascorbic acid and sugar in the fruits and its acidity. However, the yield of tomato on rockwool was higher. But the sulphate levels and type of medium did not effect the mineral composition of plants.
The aim of a study was to determine the nutrient and water uptake by tomato plant grown in three independent systems of the nutrient film technique (NFT), provided with the nutrient solutions containing different levels of sulphates: I – 200, II – 400, i III – 600 mg dm⁻³. Nutrient and water uptake depended mainly on stages of plant maturity, and in a less degree on the concentration of sulphates in the solution. Average daily uptake by single plant was (mg): nitrogen – 131.4; phosphorus – 31.6; potassium – 229.6; calcium – 100.7; magnesium –23.2. Average daily uptake of water was 1084 cm³.
A field experiments were conducted during the two summer seasons of 2015 and 2016 in saline soil (ECe = 9.0 dSm⁻¹), at Fayoum province, Egypt to study the effect of ascorbic (AsA), as foliar application alone or combined with cyanobacteria (CB) on growth, yield, its components and nutritional status of sunflower plants variety Sakha 54. Treatments comprised 2 ascorbic acid (AsA) levels with or without seed inoculation with cyanobacteria (CB). They were 1 mM AsA, 2 mM AsA, 1 mM AsA + CB and 2 mM AsA + CB in addition to the control treatment in which seeds were not received CB and their plants were sprayed with distilled water. Results could be summarized as follows: increasing the addition of ascorbic acid concentration up to 1Mm with combination of CB increased significantly values of growth attributes (e.i., plant height, of leaves no./plant, shoot dry weight and leaf area), photosynthetic pigments (chlorophyll a, b and carotenoids), physiological responses (total soluble sugars, proline and soluble phenols) as well as the head diameter, seed yield/ plant, 100 seed weight and seed yield (t ha⁻¹). Also, N, P, K, Fe, Mn, Zn and oil percentage of sunflower seeds with compared to control treatment in both years. Generally, the results in most cases, demonstrate that the all parameters increased significantly by using the concentration of ascorbic acid 1Mm combined with CB in both seasons.
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