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Interference of nematode parasitism by silencing of plant genes

100%

Koter M., Matuszkiewicz M., Baranowski L., Sobczak M., Wisniewska A., Dabrowska-Bronk J., Swiecicka M., Skowron W., Filipecki M.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2013

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tom 94

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nr 3

2

Nodule-specific repression of yellow lupin protein R18

100%

Madrzak C. J., Kulikova O., Legocki A. B.

Acta Biochimica Polonica

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1989

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tom 36

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nr 3-4

3

Characterization and expression analysis of the yellow lupin [Lupinus luteus L.] gene coding for nodule specific proline-rich protein

100%

Karlowski W. M., Strozycki P. M., Legocki A. B.

Acta Biochimica Polonica

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2000

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tom 47

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nr 2

The LlPRP2 gene coding for a proline-rich protein shows a high level of similarity to, as well as significant differences from the family of ENOD2 nodule-specific genes. Several sequence motifs with putative regulatory function were identified in the 5' and 3' noncoding regions of the LlPRP2 gene. Northern blot analysis revealed that the expression of the LlPRP2 gene begins 9 days after inoculation of yellow lupin roots with Bradyrhizobium sp. (Lupinus); the expression is restricted to symbiotic nodules and is not detected in other tissues or organs. Detailed hybridization analysis showed that, when expression is activated, the LlPRP2 transcript is modified so as to produce at least three bands and a continuous distribution of decay intermediates. The modification of the LlPRP2 transcript probably involves degradation from the 5'- and/or 3'-ends of the RNA molecules. Southern blot analysis indicates that only one gene is present in the yellow lupin genome. The presence of genes homologous to the LlPRP2 gene was confirmed for three cultivars of yellow lupin and for Lupinus angustifolius. However, LlPRP2 homologues were not detected in Lupinus albus cv. Bac, indicating that this plant may lack the ENOD2 sequence.

4

The use of RAPD and Semi-random markers to verify somatic hybrids between diploid lines of Solanum tuberosum L.

100%

Przetakiewicz J., Nadolska-Orczyk A., Orczyk W.

Cellular and Molecular Biology Letters

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2002

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tom 07

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nr 2B

Markers specific to diploid lines of cultivated potato were identified using the polymerase chain reaction (PCR), primed with arbitrary 10-mers generating RAPDs, or with semi-random primers targeting intron-exon semiconservative sequences of plant genes. One hundred and fifty RAPD primers and twelve semi-random primers were tested. Selected primers were subsequently used for verification of putative somatic hybrids.

5

Structure of yellow lupine genes coding for mitotic cyclins

100%

Jelenska J., Zaborowska Z., Legocki A. B.

Acta Biochimica Polonica

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1999

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tom 46

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nr 3

Cell cycle progression in eukaryotes is controlled by complexes of p34 protein kinases and cyclins. For the first time in plants, we have established the sequence of four yellow lupine mitotic cyclin B1 genes. Their coding regions and expression pattern were also characterised recently. Structure of all the four lupine genes is similar: they consist of nine exons and eight introns, analogously located, except Luplu;CycB1;3 lacking 7th intron. Analysis of 5˘-regulatory sequences of two of them showed that both comprise M-specific activators (MSA), common to plant genes induced in late G2 and early M. Putative repressor binding sites CDE/CHR found in animal G2-specific promoters can also be detected in lupine genes. Controlling region of Luplu;CycB1;4 gene that is highly activated by IAA, contains up to 7 auxin response elements, while insensible to IAA Luplu;CycB1;4 gene have no such motifs. Further studies should be undertaken to determine precisely the functions of putative regulatory elements in the expression of lupine mitotic cyclins.

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Using yeast two-hybrid system for identification of the proteins interacting with the product of a novel plant gene induced by sulphur deficiency stress

80%

Kaminska J., Lewandowska M., Wawrzynska A., Gaganidze D., Liszewska F., Sirko A.

Biological Letters

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2005

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tom 42

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nr 2 Spec.Vol.

7

Sequential silver staining and fish - their use to distinguish active from inactive rDNA loci

80%

Hasterok R., Maluszynska J.

Prace Naukowe Uniwersytetu Śląskiego w Katowicach

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1998

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tom 1696

8

Proba wyselekcjonowania di- oraz tetraploidalnych form jarych u Dactylis glomerata l.

71%

Stuczynski M., Stuczynska J.

Biuletyn Instytutu Hodowli i Aklimatyzacji Roślin

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1999

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tom 209

215-221

9

Charakterystyka niektorych cech ilosciowych mieszancow miedzygatunkowych F2 heksaploidalnego owsa Avena sativa L. x Avena sterilis L. oraz form wyjsciowych

61%

Paczos-Grzeda E.

Biuletyn Instytutu Hodowli i Aklimatyzacji Roślin

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2003

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tom 229

33-41

Ograniczanie wyników

Interference of nematode parasitism by silencing of plant genes

Nodule-specific repression of yellow lupin protein R18

Characterization and expression analysis of the yellow lupin [Lupinus luteus L.] gene coding for nodule specific proline-rich protein

The use of RAPD and Semi-random markers to verify somatic hybrids between diploid lines of Solanum tuberosum L.

Structure of yellow lupine genes coding for mitotic cyclins

Using yeast two-hybrid system for identification of the proteins interacting with the product of a novel plant gene induced by sulphur deficiency stress

Sequential silver staining and fish - their use to distinguish active from inactive rDNA loci

Proba wyselekcjonowania di- oraz tetraploidalnych form jarych u Dactylis glomerata l.

Charakterystyka niektorych cech ilosciowych mieszancow miedzygatunkowych F2 heksaploidalnego owsa Avena sativa L. x Avena sterilis L. oraz form wyjsciowych