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The paper describes the use of liposomes and erythrocytes membrane as a real membrane models to evaluate the potential benefits of several plants extracts and two flavones in relation to lipid peroxidation. The antioxidant behaviour of the plant extracts from pine (Pinus silvestris L), hawthorn (Crataegus oxyacantha L, two extracts: from hawthorn’s leaves-l and bark-b), evening primrose (Oenothena paradoxa Hudziok – three extracts differ in procyanidins content P1, P2 and P3) and rosemary (Rosmarinus officinalis, as a standard for extracts) and flavones of baicalin and rutin have been studied. The results obtained showed that the studied extracts exhibited differentiated, dose-dependent antioxidant activity against phospatidylcholine liposomes (rosemary>pine≈hawthorn-l>hawthorn-b≈P1≈P2≈P3; statistically significant differences were observed between the extracts at p≤0.05) and erythrocyte membranes (rosemary≥hawthorn-b≈hawthorn-l>P1≈pine>P2≈P3) when the oxidation was induced by UV-C radiation. They also reduce the oxidation of liposomes and erythrocyte membrane when its oxidation was induced by 2,2’-azobis(2-amidinopropane) dihydrochloride (pine≥P1≈P2≈rosemary≈P3 in the case of liposomes and rosemary>> pine≥P1≈P2≥P3 in a case of erythrocyte). Moreover, the results of the study show that baicalin is characterised by high inhibition ability towards liposome PC peroxidation, as well as towards erythrocyte ghosts, when oxidation was initiated by UV radiation. However, at the same experimental conditions, the inhibitory capacity of rutin was about 7-8 times weaker. The presence of cholesterol in liposome membrane decreased the level of membrane peroxidation but do not influenced on the antioxidant activity of hawthorn extract.
The aim of this work was to evaluate the antioxidant capacity and flavonoids content in 10 commercial cosmetic plant extracts used in cosmetics industry. Antioxidant activity of plant extracts were measured using two methods: FRAP (Ferric Reducing Antioxidant Power) and TEAC (Trolox Equivalent Antioxidant Capacity). The relationship between flavonoids content and antioxidant capacity of plant extracts were checked. As a result of this research it was found that FRAP and TEAC values of plant extracts significantly depend on the flavonoids content. The highest antioxidant activity, both in FRAP and TEAC assays, was observed for arnica flowers, hawthorn flowers and lungwort herb extracts. These extracts can be used as source of natural antioxidants for the prolongation of the oxidative stability of cosmetic products. Additionally, they can replace synthetic antioxidants.
The phagocyte-stimulatory properties of plants from Compositae family were studied. The extracts of Compositae family exhibit the stimulatory effect on the reticuloendothelial system. These properties depend on dose as well as the solubility of extract.
The present study was aimed to develop a cost-effective and efficient protocol for mass propagation of high-quality seedlings through tissue culture by using seaweed extract as biostimulants instead of synthetic chemicals. The nodal explant of field grown W. somnifera estabilised on Murashige and Skoogs medium (MS) and Gamborg B5 medium supplemented with six concentration of 2,4 D. The percentage of culture response from the nodal explant ranged from 44 to 80 and 3.0 mg l-1 2,4 D found to be best for callus induction. MS media containing different concentration of seaweed extract (10, 20, 40, 60, 80 and 100 %) were tested individually for shoot induction. The medium supplemented with 40 % seaweed extract exhibited maximum number of shoots with about 8.6 shoots/ callus and 80 % seaweed extract exhibited 4.3 shoots/ callus. It is evident from this study that seaweed extracts can be used as substitute for synthetic growth hormones for micropropagation of medicinally important plant W. somnifera for clonal propagation and conservation.
The pine processionary moth, Taumetopoea pityocampa (Denis and Schiffermüller) is one of the most widespread defoliator insects found in the forest areas of Turkey. Although different methods have been used to control this major forest pest up to now, the problem is still going on largely unsolved in the forest areas of Turkey. The objective of this study was to determine larvicidal effects of extracts obtained from six different plant species, Achillea wilhelmsii C. Koch, Nepeta meyeri Benth., Satureja hortensis L., Origanum onites L., O. rotundifolium Boiss., Tanacetum argyrophyllum (C. Koch) and on the 2nd, 3rd, and 4th instar larvae of T. pityocampa in laboratory conditions.Test the toxicity of six plant extracts against to the 2nd, 3rd, and 4th instar larvae of T. pityocampa, 10 larvae of this insect with 15 gr amounts fresh needles (1 year old) of Pinus brutia were placed to Petri dishes (9 × 1.5 cm deep). Each dose was dissolved in acetone and 0.25, 0.5 and 1 mg of the plant extracts found in 1 ml solution were sprayed on the 2nd, 3rd, and 4th instar larvae of T. pityocampa in the Petri dishes, corresponding to 2.08, 4.16 and 8.33 mg·l -1 air concentrations. Petri dishes were covered with a lid. All tests carried out at 26ºC (±2), 60% (±5) relative humidity and 14/10 h light/dark photoperiod in laboratory conditions. When exposure, mortality of the larvae was after the determined at 24, 48 and 96 h. Petri dish applied with sterile water and acetone were used as control group. All the tests were made in triplicate. The results showed that six plant exracts have a larvicidal effect on the 2nd, 3rd, and 4th instar larvae of T. pityocampa in comparison with controls. Therefore, these naturally occurring plant extracts could be useful for managing the larvae populations of T. pityocampa.
The polyphenol plant extracts content seems to be responsible for the scavenging activity of the reactive oxygen species (ROS), resulting in protection against DNA damage induced by the oxidative stress. This assumption was verified analyzing the effect of six Mediterranean plant extracts (Crepis vesicaria L, Origanum heracleoticum, Scandix australis L, Amaranthus sp., Scolymus hispanicus L, Thymus piperella L) on the oxidative DNA damage induced in lymphocytes by H2O2 in relation to the polyphenolic content and the lymphocyte scavenging ability of ROS. The comet assay was used to evaluate oxidative DNA damage and the polyphenol content was analyzed by the Folin-Ciocalteu method. The fluorescence resulting from oxidation of ROS-sensitive dye, dihydrorofluorescein (DHF), was utilized as indicator of the ROS level. Pretreatment with all plant extracts produced the dose-dependent increase in the DNA protection up to the 0.2 µg/ml polyphenol content and the decrease above that dose. Only the Thymus piperella, similarly to quercetin, showed a strong positive correlation between the DNA protection and the polyphenol content, but negative correlation with ROS formation. In conclusion, the DNA protective ability of plant extracts seems to be related to the low polyphenol concentration and only to certain extent depends on the polyphenol ROS scavenging activity.
Background. In the present study the antioxidant properties were investigated of the medicinal plants Salvia officinalis L. (Labiaceae family) and Viscum album L. (Loranthaceae), both of them known for a long time as a remedy in the traditional medicine. The aim of this study was to prove the efficiency of ultrafiltration process for the concentration of herbs extracts and to evaluate the concentrate‟s antioxidant activity. Material and methods. The extracts were prepared by maceration, using different sol-vents. After filtering the extract through Isolab quantitative filter paper “medium”, each of the filtrates was processed by microfiltration (MF; Millipore filters with 45 μm), followed by ultrafiltration (UF). The regenerated cellulose (Millipore), polysulfone and polyacrylonitrile ultrafiltration membranes were used in the experiment. The initial extracts and samples of permeate and retentate after ultrafiltration of extracts have been characterized by determination of the protein total and total phenolic content. Standard methods like ABTS and DPPH assay are used to measure the antioxidant activity. Results. For the three types of tested membranes: Millipore, PSF and PAN, PAN membrane proves to have the greatest efficiency since it shows the highest permeate flux and the greatest retention degree for bioactive compounds. The concentrated extracts obtained after ultrafiltration with polyacrylonitrile membrane had the strongest scavenging activity for all extracts. Conclusions. The results of this study has revealed that the concentrated extracts have a very high radical scavenging activity (TEAC values for sage hydro-alcoholic concentrated extracts in range 351.87-479.04 μmol Trolox/mL extract and for mistletoe concentrated extract E2 in range 345.14-426.18 μmol Trolox/mL extract; the DPPH inhibition values was over 85% for S. officinalis concentrated extracts and ranges between 66.2% and 88.2% DPPH inhibition for V. album concentrated), therefore can be considered as a good source for further medicinal applications.
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The current study was intended to evaluate abortifacient and antioxidant activity of Avicennia marina leaves extracts. Abortifacient activity was evaluated in rats, compared with standard drug (Mifepristone) and antioxidant activity was evaluated by using three free radicals (Superoxide, Hydroxyl and DPPH) compared with Ascorbic acid. The extracts were showed pre implantation loss, post implantation loss of implantations and decreased the survival ration of foetuses. Among all extracts hydroalcoholic extract showed better activity. The selected plant extracts showed concentration dependent percentage inhibition of free radicals. Among three extracts hydroalcoholic extract showed better activity with IC50 values on superoxide, hydroxyl and DPPH radicals were 203μg, 237μg and 143μg. From the results obtained during the study it the hydroalcoholic extract was used for the isolation of pure compounds, and isolated the different compounds (β-sitosterol, Lupeol and Betulinic acid).
The aim of our work was to investigate the antioxidant activity of Rheum palmatum extracts. Antiradical activity against DPPH and ABTS radicals, reducing power FRAP and total phenolic contents, were investigated in one-, two- and three-year-old roots of rhubarb fertilized with nitrogen at the rates of 50, 100 and 200 kg N/ha. It was proved that nitrogen dose as well as the age of plantation did significantly influence antioxidant activity and total phenolic of root extracts. The highest values were determined in one-year-old plants, antioxidant activity ranged the level of 112–203 μM Trolox/g and total phenolic compounds average content was 22 mg GAE/g FW. Two-year-old roots were characterized by 3–11%, and three-year-old ones by 15–23% lower antioxidant activity and phenolic compounds content. When nitrogen dose increased, polyphenols content, DPPH and FRAP values increased as well, although, ABTS showed a different tendency.
The aim of this study was to evaluate the effect of different fractions of the aqueous crude extract of Detarium senegalense stem bark on castor oil-induced diarrhea. Castor oilinduced diarrhea, gastrointestinal motility and castor oil-induced enteropooling methods were used to evaluate the antidiarrheal effects of the fractions. Castor oil was used to induce diarrhea and the effect of all the fractions (chloroform, ethyl acetate, n-butanol, methanol and residual aqueous) were evaluated at the doses of 200 and 400 mg/kg body weight. The results show that all fractions significantly (p<0.05) decreased the frequency of defecation in rats following the induction of diarrhea with castor oil. Ethyl acetate which produced the highest antidiarrheal activity was further subjected to gastrointestinal motility and castor oil-induced enteropooling tests. In the gastrointestinal motility, two test doses of the extract (200 and 400 mg/kg) were administered orally to two groups of rats (n=5), while the third group of rats (control), were treated with normal saline, and the fourth group were treated with diphenoxylate, a conventional anti-diarrheal drug. In the castor oil-induced enteropooling experiment, normal saline was used for the control animals, while 200 and 400 mg/kg of the extract was administered to groups two and three, respectively and atropine, a standard drug, was administered to rats in group four. The ethyl acetate fraction significantly (p<0.05) decreased the gastro-intestinal motility, as shown by the extent of movement of the charcoal meal in the treated rats. It also significantly inhibited the fluid accumulation within the intestine. These findings suggest that the ethyl acetate fraction possess antidiarrheal effect, which may be due to the presence of some phytochemical constituents (alkaloids, flavonoids and tannins) in the plant, which may either be working alone or in combination with each other. This study has demonstrated that D. senegalense fractions, especially the ethyl acetate fraction, possess antidiarrheal activity thus supporting the use of the plant in the treatment of diarrheal diseases.
Using the general Hildebrand-Scatchard-Fedors theory of solubility, the mole fraction (x2) of solubility of phytochemicals contained in the dry green tea leaves was calculated which determines the profile of pharmacological activity.The applicative purpose of the study was to estimate the actual solubility of phytochemicals – S|real.| [mol/dm3] in water and in water-ethanol solutions of diversified polarity (εM) for their selective extraction and optimal formulation of oral solid dosage form.The basic physico-chemical and structural quantities of phytochemicals and corresponding mathematical equations of general Hildebrand-Scatchard-Fedors theory of solubility were used to calculate the actual solubility – S|real.| and the level of hydrophilic-lipophilic balance (HLB).The calculated actual solubility values – S|real.| [mol/dm3] collated with correlation equations enabled the assessment of phytochemical capability for the process of mass exchange on phase boundary. Correlation equations for the dependence log P = f (– log S|real.|) point to the structural preferences of phytochemicals in the kinetics of the mass exchange (diffusion) through the natural phase boundary.Calculations and correlations between the values characterizing the actual solubility – S|real.|, media polarity (water, ethanol and their solutions) and the partition coefficient (log P) including the level of hydrophilic-lipophilic balance (HLB) show that basing on thermodynamic components of the general Hildebrand-Scatchard-Fedors theory of solubility, the diffusion profile of phytochemicals contained in the green tea extract (Ext. Camellia sinensis L. aqu. siccum) through the biological phase boundary as well as optimal choice of the extraction medium for selective extraction of the class of phytochemicals can be estimated.
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