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1

Biotechnological plant improvement

100%
Szopa J.
Polish Journal of Natural Sciences. Supplement
|
2003
|
tom 01
2

Plant selectable markers and reporter genes

88%
Ziemienowicz A.
Acta Physiologiae Plantarum
|
2001
|
tom 23
|
nr 3
3

Biotechnology of temperate fruit trees and grapevines

88%
Laimer M., Mendonca D., Maghuly F., Marzban G., Leopold S., Khan M., Balla I., Katinger H.
Acta Biochimica Polonica
|
2005
|
tom 52
|
nr 3
Challenges concerning fruit trees and grapevines as long lived woody perennial crops require adapted biotechnological approaches, if solutions are to be found within a reasonable time frame. These challenges are represented by the need for correct identification of genetic resources, with the foreseen use either in conservation or in breeding programmes. Molecular markers provide most accurate information and will be the major solution for questions about plant breeders rights. Providing healthy planting material and rapid detection of newly introduced pathogens by reliable methods involving serological and molecular biological tools will be a future challenge of increases importance, given the fact that plant material travels freely in the entire European Union. But also new breeding goals and transgenic solutions are part of the biotechnological benefits, e.g. resistance against biotic and abiotic stress factors, modified growth habits, modified nutritional properties and altered processing and storage qualities. The successful characterization of transgenic grapevines and stone fruit trees carrying genes of viral origin in different vectors constructed under ecological consideration, will be presented. Beyond technical feasibility, efficiency of resistance, environmental safety and Intellectual Property Rights, also public acceptance needs consideration and has been addressed in a specific project. The molecular determination of internal quality parameters of food can also be addressed by the use of biotechnological tools. Patient independent detection tools for apple allergens have been developed and should allow to compare fruits from different production systems, sites, and genotypes for their content of health threatening compounds.
4
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

The untapped potential of plant thin cell layers

75%
Teixeira da Silva J., Altamura M. M., Dobranszki J.
Journal of Horticultural Research
|
2015
|
tom 23
|
nr 2
5

progress in production of drugs in transgenic plants

75%
Budzianowski J.
Herba Polonica
|
2007
|
tom 53
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nr 2
6

cDNA arrays and PCR-select applied for characterization of agricultural features in plants

75%
Podkowinski J., Zmienko A., Bejger M., Figlerowicz M., Legocki A. B.
Journal of Fruit and Ornamental Plant Research
|
2006
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tom 14
|
nr Suppl.1
7

Plant regeneration via somatic embryogenesis in Pinus kesiya [Royle ex.Gord.] influenced by triacontanol

75%
Malabadi R. B., Mulgund G. S., Nataraja K.
Acta Physiologiae Plantarum
|
2005
|
tom 27
|
nr 4A
Embryogenic cultures were initiated and established for the first time in 3 different genotypes of Pinus kesiya using mature zygotic embryos and triacontanol. Mature zygotic embryos produced white-mucilaginous embryogenic callus when cultured on half strength MSG (Becwar et al. 1990) basal medium supplemented with 90 mM maltose, 2.0 ·g l⁻¹ Gellan gum, 9.0 μM 2, 4-D and 10 μg·1⁻¹triacontanol. On subculture of such embryogenic callus on the maintenance medium (II) containing 2.0 μM 2,4-D and 2.0 μg ·l⁻¹ triacontanol induced cleavage polyembryogenesis with proembryos. The percentage of somatic embryogenesis was not similar in all the three genotypes. The highest percentage of somatic embryogenesis (88.5 %) was recorded in PK04 genotype. Somatic embryos were successfully germinated on half strength MSG basal medium without growth regulators. Somatic seedlings showed fast growth and a survival rate of 95 %. This work for the first time reveals that triacontanol can be used as an effective growth regulator for inducing somatic embryogenesis in conifers.
8
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Content available

International conference Agrobiotechnology'95. Center of Science, Polish Academy of Sciences, September 17-20, 1995, Poznan, Poland

75%
Twardowski T.
Journal of Applied Genetics
|
1995
|
tom 36
|
nr 4
9
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Coccinia abyssinica (Lam.) Cogn. (anchote) biology, productivity, and prospects of genetic improvement using biotechnological tools

63%
Feyissa T.
Journal of Horticultural Research
|
2020
|
tom 28
|
nr 2
10
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Recent advances and novelties in the thin cell layer-based plant biotechnology – a mini-review

63%
Da Silva J. A. T., Dobranszki J.
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
|
2019
|
tom 100
|
nr 1
11

Modifications in acid growth and cell wall proteins of soybean shoots [Glycine max L. Merr] induced by the reduction of blue light content in sunlight

63%
Urysiak P. E.
Polish Journal of Natural Sciences. Supplement
|
2003
|
tom 01
12

Development of an efficient retrotransposon-based fingerprinting method for rapid pea variety identification

63%
Smykal P.
Journal of Applied Genetics
|
2006
|
tom 47
|
nr 3
Fast and efficient DNA fingerprinting of crop cultivars and individuals is frequently used in both theoretical population genetics and in practical breeding. Numerous DNA marker technologies exist and the ratio of speed, cost and accuracy are of importance. Therefore even in species where highly accurate and polymorphic marker systems are available, such as microsatellite SSR (simple sequence repeats), also alternative methods may be of interest. Thanks to their high abundance and ubiquity, temporary mobile retrotransposable elements come into recent focus. Their properties, such as genome wide distribution and well-defined origin of individual insertions by descent, predetermine them for use as molecular markers. In this study, several Ty3-gypsy type retrotransposons have been developed and adopted for the inter-retrotransposon amplified polymorphism (IRAP) method, which is suitable for fast and efficient pea cultivar fingerprinting. The method can easily distinguish even between genetically closely related pea cultivars and provide high polymorphic information content (PIC) in a single PCR analysis.
13

The role of plant growth regulators on direct and indirect plant regeneration from various organs of Leucaena leucocephala

63%
Maity S., Ray S., Banerjee N.
Acta Physiologiae Plantarum
|
2005
|
tom 27
|
nr 4A
Prolific differentiation of shoot buds of Leucaena leucocephala was induced from the different plant parts viz. cotyledon, hypocotyl and leaf. Adventitious shoot bud formation was recorded with prudent application of N6-2- (isopentenyl) adenosine and 15 % (v/v) coconut water. Coconut water alone was unable to produce any beneficial effect with regard to the shoot bud proliferation but the response was augmented with the increase in concentration of N6-2- (isopentenyl) adenosine. However supra-optimal level of N6-2- (isopentenyl) adenosine was inhibitory. Best response was recorded from the cotyledon explant at 2 mg·dm-3 N6-2- (isopentenyl) adenosine compared to the other two explants. Comparative assessment was undertaken following the same experimental protocol in liquid shake culture. The regenerated shoot buds were subcultured in plant growth regulator-free medium where leafy shoot emergence was recorded. Optimum regeneration of roots was observed in these shoots in presence of 1 mg dm-3 α-naphthalene acetic acid. Plantlets were finally hardened following standard procedures before transplantation to the field. In another experimental set up, the de-embryonated cotytedons regenerated shoot buds via callus formation. The regenerated shoots and plantlets obtained through callus mediated organogenesis could be used for rapid multiplication and also for the genetic improvement of individual clones of Leucaena leucocephala.
14

Efficient in vitro plant regeneration of Pinellia ternata [Thunb] Breit

63%
Xu T., Zhang L., Sun X., Tang K.
Acta Biologica Cracoviensia. Series Botanica
|
2005
|
tom 47
|
nr 2
An efficient plant regeneration system for Pinellia ternata (Thunb) Breit, an important wild Chinese herb, was established. The investigated factors influencing callus induction and plant regeneration included sterilizing agents, explant type, culture medium, and combinations of plant growth regulators in the medium. Ten min treatment of explants with 0.1% HgCl2 was more suitable for sterilization of tuber explants than NaCIO and H2O2. Tubers as explants were superior to leaves and petioles, and MS medium was better than N6 and White’s media. It was found that 9.1 µM 2,4-dichlorophenoxyacetic acid (2,4-D) plus 4.4 µM 6-benzyladenine (BA) in the medium was the best hormone combination for promoting callus induction. The highest shoot regeneration frequency (99%) was achieved from callus cultured on MS medium supplemented with 9.3 µM kinetin (KN) and 2.3 µM 2,4-D. The rooted shoots were successfully transplanted in soil, with over 95% survival. Establishment of an efficient plant regeneration procedure provides a basis for rapid in vitro propagation of P. ternata, which is in great demand in China, and represents an excellent tool for further work on its genetic manipulation.
15
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Hiring cell gatekeepers – ABC transporters in plant biotechnology

51%
Szewczak A., Ziomkiewicz I., Jasinski M.
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
|
2011
|
tom 92
|
nr 2
16
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

A green light for plants

51%
Jasinski M.
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
|
2011
|
tom 92
|
nr 2
17
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Role of biotechnology in developing the bioenergy sector in Poland

51%
Daroch M., Mos M.
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
|
2011
|
tom 92
|
nr 1
18

Wykorzystanie biotechnologii przez polską hodowlę roslin

51%
Zimnoch-Guzowska E., Golebiewska M.
Biuletyn Instytutu Hodowli i Aklimatyzacji Roślin
|
2011
|
nr 259
19
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Activity of hygromycin phosphotransferase marker gene optimized for expression in plants for the construction of vectors for genetic modifications of grasses

51%
Malec K., Kowalska M., Podkowinski J., Zielinska M., Cerazy J., Slusarkiewicz-Jarzina A., Ponitka A., Jezowski S., Pniewski T.
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
|
2015
|
tom 96
|
nr 1
20

Induction and characterization of streptomycin-resistant mutants in Capsicum praetermissum

51%
Venkataiah P., Christopher T., Subhash K.
Journal of Applied Genetics
|
2005
|
tom 46
|
nr 1
Streptomycin-resistant mutants were isolated from mutagenised cotyledon explants of Capsicum praetermissum Heiser & Smith. The expiants were mutagenised with N-ethyl-N-nitrosourea, which resulted in a high frequency of streptomycin-resistant mutants (18.0%) and a low frequency of chlorophyll-deficient (albino) mutants (8.0%). Complete streptomycin-resistant plantlets were obtained after rooting of the regenerated green shoots on rooting medium containing 1.0 mg L⁻¹ IAA and 500 mg L⁻¹ streptomycin sulphate. Leaf-segment assay of these plantlets revealed that they were resistant to streptomycin but sensitive to chloramphenicol, kanamycin, lincomycin, and spectinomycin. Reciprocal crosses between streptomycin-resistant and -sensitive plants showed a non-Mendelian transmission of resistance by female parents.
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