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The aim of the study was to analyze the distribution of calcium ions in the pig pineal gland at the level of electron microscopy. The investigations were performed on the pineals obtained immediately after slaughter (performed between 11:30 and 12:00 a.m.) from 4-month-old gilts. The fixation procedures were conducted with the use of pyroantimonate, which reacted with calcium ions and formed electron dense precipitates. The precipitates were found both in the intercellular spaces and in cells - pinealocytes, gial cells, endothelial cells and fibroblasts. The precipitates were much more numerous in the intercellular spaces than in the cells. Amount and distribution of precipitates differed significantly between pinealocytes, therefore two types of cells were distinguished. The first type of pinealocytes included cells containing a small or moderate amount of precipitates. They were usually characterized by light or dark cytoplasm and large variability in number and structure of dense bodies. Pinealocytes classified to the second type possessed large or very large content of precipitates. These cells were characterized by electron dense cytoplasm and showed the presence of numerous dense bodies. In both types of pinealocytes, precipitates were present in the nucleus and in the cytoplasm. In nuclei, precipitates were numerous in nucleoplasma and rather infrequently noted between membranes of the nuclear envelope. In the cytoplasm deposits were found in mitochondria, vesicles and cisterns of smooth endoplasmic reticulum, in the Golgi apparatus and in cytosol. The amount of precipitates in glial cells, endothelial cells and fibrocytes was lower than in pinealocytes.
The pinealocytes of the pineal gland of children and adults were studied at both light and electron microscopic levels. The pinealocytes were classified into light and dark pinealocytes on the basis of their shape, nuclear infolding, cytoplasmic contents and staining density. The light pinealocytes outnumber the dark pinealocytes and both of them have thin processes. The light pinealocytes have round or oval cell bodies and nuclei and have vesicles and ribbons. The dark pinealocytes showed more variations in their shape. The nuclear membrane of the dark cells showed numerous infoldings with deep invagination of parts of the cytoplasm within the nuclear folds, giving the appearance of nuclear pellets. The dark pinealocytes contain pigment in their cytoplasm. In addition to the light and dark pinealocytes a very small cell type with an extremely thin and elongated cell body and nucleus was found. The cells of this type were almost always associated with vacuoles filled with flocculent material and accumulations of presumptive secretion in the extracellular compartment. The findings of this study were discussed in the light of the published data about the pinealocytes of human and non-human species.
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Malatonin in humans

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Melatonin, the hormone of the pineal gland, received a great deal of attention in the last decade because of its availability as over-the-counter drug or food supplement in some countries and suggested role in many vital physiological processes. Melatonin secretion is not restricted to mammals but is also produced in nonmammalian vertebrates, in some invertebrates, and in many plants, with the same molecular structure. The synthesis of melatonin is strictly controlled by lighting conditions and shows a clear circadian rhythm with low values during the daytime and significant increase at night. In this survey the basic data on melatonin significance in human physiology and in pathological processes as well as its possible thrapeutic significance are reviewed and discussed.
The Ca2+ -dependent activation of retina-specific guanylyl cyclase (retGC) is medi­ated by guanylyl cyclase-activating proteins (GCAPs). Here we report for the first time detection of a 19 kDa protein (p19) with GCAP properties in extracts of rat retina and pineal gland. Both extracts stimulate synthesis of cGMP in rod outer segment (ROS) membranes at low (30 nM) but not at high (1 uM) concentrations of Ca2+ .AtlowCa2+ , immunoaffinity purified p19 activates guanylyl cyclase(s) in bovine ROS and rat reti­nal membranes. Moreover, p19 is recognized by antibodies against bovine GCAP1 and, similarly to other GCAPs, exhibits a Ca2+ -dependent electrophoretic mobility shift.
Assays, made on 64 mature carp females aged 5 years, were performed during the spawning season (summer) and in mid-winter. Some fish were subject to intraventricular melatonin microinjections, while other had their pineal gland excised. Intensity of fluorescence in the hypothalamic aminergic nuclei was determined with the fluorescence histochemical method. The lowest fluorescence intensity was revealed in those individuals lacking the pineal gland, the highest intensity being typical of the fish subject to intraventricular melatonin microinjections. In the winter series, all the fish showed a similar fluorescence intensity in the hypothalamic region studied. The results demonstrate a relationship between the pineal gland, melatonin, and the hypothalamic aminergic system, present in carp during the spawning period.
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