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The diversity of ectomycorrhizal fungi and sporocarps abundance were investigated in 2003-2005 at nine permanent study plots in a 50-year-old pine forest. Diversity of ectomycorrhizal fungi consist of 53 taxa and the majority of them belonged to the genera Cortinarius, Russula, Amanita and Tricholoma. The most frequent species, whose fruit bodies were found in each study plot, were C. cibarius, L. necator L. rufus, P. involutus, R. aeruginea, T. saponaceumand the most abundant species which made the main part of total sporocarp yield were C. cibarius and P. involutus. The lowest species richness of ectomycorrhizal fungi was in study plots with the densest cover of grasses. Maximum of species over the fruiting period was characteristic for October and for September. It was noticed that some species virtually never occurred together at the same plot (e.g. C. cibarius and H. aurantiaca), meanwhile others occurred together quite frequently (e.g. H. aurantiacaand X. badius).
A Scots pine forest, affected by the great cormorant colony, was studied by plot-based fungal survey method during the years 2010-2012 in Lithuania. Diversity and composition of fungal communities were investigated at five zones that had been influenced by different stages of breeding colony establishment: starting-point and almost abandoned cormorant colony part (zones A and B), active part (zones C and D), and the edge of the colony (zone E). The control zone G in undamaged by cormorants pine stand was assessed too. A total of 257 fungal species of ascomycetes including anamorphic fungi, basidiomycetes and zygomycetes were recorded. Seven species were registered for the first time in Lithuania. Species richness in the examined zones varied, lowest being in zones B (51 species), C (46) and D (73) and almost twice as high in the zones A and E (129 and 120, respectively). The comparison of fungal species compositions of different zones showed that their similarity was rather low (SS: 0.22–0.59). The most obvious changes in the trophic structure of fungal communities in the territory occupied by the bird colony were a strong decrease of mycorrhizal species, the presence of coprophilous fungi on forest litter, and the appearance of host-specialized fungi on alien and non-forest plants that have established in the disturbed forest.
Our research was conducted on abandoned fields which had been undergoing spontaneous succession for 40–50 years and then were partly burnt. The main objective of the study was to examine if spontaneous fire in the early successional stage of pine forest causes a decrease in the number of species, diversity and density of seed banks, and an increase in the share of species forming long term persistent seed banks. Standing vegetation and soil seed banks were studied on 20 permanent plots on adjacent burnt and unburnt sites one and twelve years after spontaneous fire. One year after the fire the number of species in the seed banks of both areas was similar (11 and 12 species). In the burnt area the Shannon index and the floristical similarity between the seed bank and standing vegetation were lower, and seed density five times higher than in the unburnt area (15 691.5 m–2 vs.3426.5 m–2). This was mainly the effect of the high number of seedlings of Calluna vulgaris (L.) Hull and Spergula morisonii Boreau germinating from the burnt plots. Twelve years after the fire the number of species in both seed banks had increased little, but seed density in the burnt area (1742.5 m–2) had decreased 16 times, while that of the unburnt area had changed only slightly (2875.5 m–2). At the same time the Shannon index for the burnt area increased considerably, whereas for the unburnt one it did not change. Our study shows that the long-term persistent soil seed bank plays a fundamental role at the beginning of the post-fire regeneration of temperate coniferous forest vegetation. Germination of Calluna seedlings from the burnt soil seed banks in several times higher numbers than from unburnt soil seed banks may suggest that heat from the fire promotes a loss of dormancy in the heather seeds.
We assessed the assemblages of birds inhabiting pine-dominated managed forest, aged between 1–5 years and above 140 years. Birds were counted on study plots representing eight different phases to the forest's development, with nesting, foraging and migratory guild categories recognised. Numbers of bird species and population densities, both increased markedly with stand age (respectively r = 0.988 and r = 0.936, P < 0.001). While numbers of ground-nesting bird species failed to correlate with stand age, successively older stands did support ever-greater proportions of species in the assemblage that nested in tree crowns (r = 0.976, P < 0.005) or tree holes (r = 0.833, P < 0.005). Raptors were most abundant in forest at the oldest stages of growth, and there was an age-gradient-related increase in the shares of both plant-eating species (r = 0.952, P < 0.005) and raptors (r = 0.764, P < 0.005). Resident birds were most numerous in the oldest forest. PCA for ten selected variables (relating to guild type) showed that the two principal components explained almost 98% of the variation among groups of bird guilds in relation to forest age. On the basis of their suitability for birds it was possible to distinguish three categories of stand by age group, of which the first encompasses the initial stage, the second a broad interval involving middle-aged stands of between 16 and 140 years, and the third forest more than 140 years old. The presence of the oldest stands of all can thus be seen to play a very important role in maintaining high-diversity populations of birds in managed forest.
The informative power of species surrogacy with respect to ecological processes and anthropogenic influences has been rarely studied. Thus, five datasets on carabid beetles collected using pitfall traps were analysed in order to study the impact of changing the taxonomic resolution from species to genus level on their indicatory information: a dataset of eight study sites in differently managed habitats sampled in 2013, a dataset tracing successional changes from 2004 to 2013 in a naturally regenerated pine forest, a dataset of three sites on a heap of power plant ashes and a dataset of four sites on a colliery spoil heap, both sampled from 2004 to 2011, and a dataset of six sites along the roadside of a highway being renovated in 2009, sampled in 2008 and from 2010 to 2012. The datasets were analysed by studying correlations of species numbers with genus numbers and species based Shannon diversity with genus based Shannon diversity, testing compliance between species based and genera based similarity matrices, and comparing the information provided by ordination diagrams based on species information or genus information respectively. The results indicate that at least in our study a substantial amount of information provided by species data is still contained in the genus data, but information about fine graded differences between study sites gets lost. We conclude that, even if carabid genus information might be useful in some cases (e.g. preliminary biodiversity assessment), the limitation to higher taxonomic levels like the genus level has to be done with caution.
The recovery of species composition typical for ancient forests in recent woods is a very slow process and may last for decades or even centuries. It is enhanced only when postagricultural woods are adjacent to ancient ones. However, even in such a situation of the spatial contact of both forest types, colonization of recent woods by true forest species is a gradual process. According to studies focusing on the behaviour of individual species and their colonization rates into recent woods, it can be concluded that in more fertile habitats the migration process proceeds faster than on poorer sites. Thus, studies were conducted on light, acidic soils both in ancient and in adjoining post-agricultural pine woods (the Dicrano-Pinion Libb. 1933 alliance) and were focused on the process of the colonization of the herbaceous layer by woodland flora in recent woods. In eight transects 80 m in length perpendicular to the ancient/recent ecotone and consisting of 10 sample plots of 16 m2 laid out at intervals of 4 m, the percentage cover of herb layer species was recorded. The migration rates (based on the occurrence of the farthest individual and on the occurrence of the maximum cover of a species) for 12 forest species were calculated. The mean migration rate for all species reached 0.54 m yr–1 when based on maximum cover and 0.67 m yr–1 when based on the farthest individual and appeared to be lower than those reported in investigations in more fertile and moister habitats. The migration rates for individual species ranged from 0 to 1.21 m yr–1 and were also lower than in more fertile, black alder woodlands. The migration pattern of Vaccinium myrtillus L., the most abundant species in pine woods, fits the model based on the establishment of isolated individuals. The cover of most woodland species increased with the increasing age of a recent wood. Herb layer recovery on such sites is slower than in the more productive, fertile habitats of broadleaved forests. The ancient and recent pine woods investigated here differed in herb layer species composition despite the secondary succession having lasted for over 50–60 years.
Temporal variation is a major source of the uncertainty in estimating the fluxes of the greenhouse gases (GHGs) in terrestrial ecosystems, and the GHG fluxes and its affecting factors in the karst region of southwest China remains weakly understood. Using the static chamber technique and gas chromatography method, the CO₂, CH₄ and N₂O fluxes were carried out between 9 and 11 a.m. at 15 day intervals from June 2008 to May 2009 in a Pinus massoniana forest. Two treatments were chosen for this study: undisturbed (soil with litter layer) and disturbed (surface litter removal). Both treatments were found to be the net source of atmospheric CO₂ and N₂O, but a sink of atmospheric CH₄. The seasonality of soil CO₂ emission coincided with the seasonal climate pattern, with high CO₂ emission rates in the hot-wet season and low rates in the cool-dry season. In contrast, seasonal patterns of CH₄ and N₂O fluxes were not clear, although higher CH₄ uptake rates were often observed in autumn and higher N₂O emission rates were often observed in spring (dry-wet season transition). The litter was active in GHG fluxes, and removal of the litter layer reduced soil CO₂ emission (17%) and increased CH₄ uptake (24%) whereas N₂O fluxes were not affected distinctly in the pine forest, indicating that litter layer had an important effect on C exchanges. In the pine forest, soil CO₂ emissions and CH₄ uptakes correlated significantly with soil temperature (r²= 0.87, P <0.01; r²= 0.34, P <0.05, respectively), but had no significant relationship with soil moisture. And there was a significant correlation between CH₄ flux and NH₄⁺-N (r²= 0.39, P < 0.05) and soil inorganic N (r²= 0.48, P <0.05), but no significant correlation was found between CH₄ flux and NO₃⁻-N. Moreover, we found a significant negative logarithmic correlation between N₂O flux and soil NO₃⁻-N concentration (r²= 0.41, P <0.05), and the relationship between CO₂ emission and soil inorganic N content (r²= 0.35, P < 0.05). These results suggested that soil temperature and mineral N dynamics largely affected the temporal GHG exchanges between forest soil and atmosphere.
Initial populations of club mosses were investigated in pine forests of southern Lithuania (ass. Cladonio-Pinetum and Vaccinio vitis-idaeae-Pinetum). Results show that populations of lycophyta gametophytes in some forest sites can be abundant (up to 500 individuals in a square meter). All juvenile populations were found in localities where recorded tree canopy cover was 30% or less. Localization of subterranean gametophyte populations was determined by juvenile club mosses. We mapped locations of all Lycopodium L. and Diphasiastrum Holub. juvenile sporophytes and gametophytes that occurred within three randomly selected and excavated 0.25 m² sample plots at four pine forest sites in different localities in southern Lithuania. The developmental stage of each individual was recorded. Eight out of twelve soil samples from four research localities contained subterranean gametophytes. In total 277 gametophytes and 124 juvenile sporophytes were recorded. The developmental stage and size of the gametophytes varied. Gametophytes were divided into six categories on the basis of external features. Spatial distribution of gametophytes and juvenile sporophytes were evaluated using a nearest neighbor analysis (NNA). Our results suggest that initial populations of gametophytes in pine forests are multi-aged and display a relatively stable degree of spatial aggregation. Greater aggregation occurred among juvenile sporophytes.
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Pine wilt disease caused by pine wood nematodes is endemic to North America. Pine wood nematodes have already spread to East Asia, including China, Taiwan and Korea in addition to Japan. The pine wood nematode, Bursaphelenchus xylophilus, was discovered in Portuguese pine forests in 1999. If it were to become established in the pine forests in Europe, it could become one of the most serious threat to coniferous forests worldwide.
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