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In the present work the impact of the 3-pyridyl ketoxime and its quaternary pyridinium bromides with various alkyl chain lengths on the monocot plant – maize (Zea mays L.) was tested. The study was carried out on two systems – hydroponic and soil. After a seven-day period of these experiments, the number of germinated seeds was counted and the length of maize radicle and shoot was also measured. 3-pyridyl ketoxime and pyridinium ketoxime bromides had varying effects on the germination of maize seeds and the development of radicle and shoot. Toxicity of these compounds depended largely on the structure of the compound, and the systems on which the plant was grown. The toxic effects of tested compounds on maize development were higher in hydroponic systems compared with the soil system.
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Electrolyte efflux, chlorophyll, malondialdehyde content and enzymatic activities (catalase, peroxidase) in cucumber cotyledons as well as stability of model lipid membranes in the presence of some new, acyclic and cyclic, aminophosphonates were studied. They also differed in the substituents at the phosphorus and nitrogen atoms. It was found that the effectiveness of the compounds to influence mentioned parameters depended on their lipophilicity and structural features. The most active compounds were these with isopropyl substituents at the P atom unless their hydrocarbon chains at the N atom were not too long. Butyl substituents at phosphorus significantly decreased aminophosphonates efficiency. Similar effect was observed when trans-type hydrocarbon chain was replaced with a short-branched one. It also seems that cyclic compounds with hexane rings were weaker modifiers of model and biological membranes than these with pentane rings. Results show that aminophosphonates interact with plasma membranes and presumably induce oxidative stress.
Manganese contents in various extracts (2 M HN03, 0.05 M EDTA and 0.01 M CaCl2) and total Mn content in soil samples of various soil types from 6 regions of Slovakia were determined. After simulated loading the Mn extraction in 2 M HN03 decreased in order Lgp > Hcf > Jc Chc > Lga > Rc. The same order was also in 0.05 M EDTA and 0.01 M CaCl2. The results showed that Mn extractability is significantly determined by physicochemical soil properties (soil reaction, humus content and quality). Acid soils with a low humic acid content and high fulvoacid content appeared to be the highest risk soils.
The toxic effects of new quaternary ammonium chlorides on dicotyledonous plant Sinapis alba was evaluated. Investigated compounds differed either in hydrophobic chain length or hydrophilic part properties. The compounds used in the study are meant to be new wood preservatives because of their excellent fungicidal properties. It has been stated that the toxic effects of examined chlorides on Sinapsis alba depend on the compound structure. Alkyl(alkoxymethyl)dimethylammonium chlorides had the lowest phytotoxic properties. The presence of benzene subsituent in the compound molecule caused an increase in toxic effects of ammonium chlorides on the Sinapis alba roots.
The activity of phytotoxic lithium was different on different soils. Lithium affected plant development, plant appearance and yield; in the first year of the experiment its toxic effect was more pronounced than in the second.
The aim of this study was to compare the physiological responses of six plant species (popular crops or plants recommended as indicators of soil pollution) to a wide range of glyphosate concentrations (0, 1, 3, 7, 10, 40, 80, 120, 180, 240, 400, 750, 1,000, 1,500, 1,700 and 2,000 μM). Percent germination, root length, seedling dry mass and myo-inositol content, as well as seedling leachate electroconductivity were determined in Lepidium sativum, Sinapis alba, Sorghum saccharatum, Brassica napus, Lupinus luteus and Avena sativa. Percent seed germination, seedling dry mass and electroconductivity of seedling leachates were not clearly affected by the herbicide and could not be used as indicators of its phytotoxicity. An metabolite induced by abiotic stresses in many plants, myo-Inositol, was very strongly stimulated by glyphosate at doses above 10 or 40 μM, depending on plant species. The sensitivity of analyzed plants to glyphosate, as manifested by root length, differed clearly. In Avena sativa the relationship between root length and glyphosate concentration was fairly linear over a wide range of herbicide doses (up to 240-400 μM). The most distinct drop in root growth at low herbicide doses was visible in Sorghum saccharatum. The results show that a mild stress affecting root length may not clearly modify seedling myo-inositol levels, that respond distinctly to stronger stresses. Not all indicator plants are equally suitable for analysis of biological activity of glyphosate residues. Sorghum saccharatum seems particularly sensitive.
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