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1

Influence of culture conditions of Streptomyces sp. [strain S242] on chitinase production

100%
Saadoun I., Al-Omari R., Jaradat Z., Ababneh Q.
Polish Journal of Microbiology
|
2009
|
tom 58
|
nr 4
339-345
The purpose of this study was to determine the influence of growth conditions and medium composition on the production of chitinase by Strtptomyces sp. (strain S₂₄₂,). Production of chitinase by strain S₂₄₂ was detected on colloidal chitin agar (CCA) medium after 8 days of incubation at 28°C resulting in a clear zone 10 mm around the colony. Chitinase activity was assayed as the amount of N-acetylglucosamine released in pmol/ml/min using the dinitrosalicylic acid assay method. The crude enzyme had maximum activity (0.162 U ml/l) after 4 days of incubation at pH 7 and 30°C when the broth medium was supplemented with 1.6% of colloidal chitin. However, enzyme activity was strongly decreased at 40°C and extreme acidic and alkaline pH values. SDS-PAGE and zymogram analysis revealed six distinctive bands that range from 39 to 97 kDa with chitinolytic activity. The findings of this investigation create a possibility for the use of the organism in the commercial production of chitinase. In addition, it can be a source of DNA for cloning the chitinase gene(s) to generate phytopathogen resistant transgenic plants.
2
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Protective effect of antagonistic microorganisms in limiting the occurrence of certain pathogenic soil-borne fungi

100%
Patkowska E.
Acta Agrophysica
|
2003
|
tom 01
|
nr 1[86]
3
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Effect of Miedzian 50 WP and grapefruit extract on the healthiness and communities of soil microorganisms of pea (Pisum sativum L.)

84%
Patkowska E.
Acta Scientiarum Polonorum. Hortorum Cultus
|
2014
|
tom 13
|
nr 3
W badaniach określono wpływ ekstraktu z grejpfruta i fungicydu Miedzian 50 WP na zdrowotność grochu oraz na kształtowanie się populacji mikroorganizmów w ryzosferze tej rośliny. Liczba roślin wyrosłych z nasion zaprawianych Grevitem 200 SL bywa zbliżona, a nawet przewyższa, ale nie statystycznie istotnie, liczbę roślin uzyskanych po zastosowaniu fungicydu Miedzian 50 WP. Wartość indeksu porażenia roślin była najmniejsza po wykorzystaniu Grevitu 200 SL, ale statystycznie nie różniła się od tej dla Miedzianu 50 WP. Z porażonych roślin izolowano m. in. Alternaria alternata, F. culmorum, F. oxysporum, F. solani, Phoma eupyrena, Pythium irregulare, Rhizoctonia solani i Sclerotinia sclerotiorum. Z ryzosfery izolowano m. in. Alternaria alternata, Fusarium spp., Gliocladium spp., Mucor spp., Penicillium spp., R. solani, S. sclerotiorum i Trichoderma spp. Liczebność bakterii ryzosferowych w kombinacjach z Grevitem 200 SL i Miedzianem 50 WP byáa istotnie większa, aniżeli w kombinacji kontrolnej. Odwrotna zależność wystąpiła w przypadku liczebność grzybów, ale statystycznie mniej grzybów wystąpiło w kombinacji z Grevitem 200 SL. Mikroorganizmy antagonistyczne dominowały w ryzosferze roślin wyrosłych z nasion zaprawianych Grevitem 200 SL.
4
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Isolation and molecular identifcation of laccase-producing saprophytic/ phytopathogenic mushroom-forming fungi from various ecosystems in Michoacan State, Mexico

84%
Mukhtar I., Arredondo-Santoyo M., Vazquez-Garciduenas S., Vazquez-Marrufo G.
Acta Mycologica
|
2019
|
tom 54
|
nr 1
5
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Antifungal activity of Solanum extracts against phytopathogenic Curvularia lunata

84%
Hernandez-Rodriguez Z. G., Riley-Saldana C. A., Gonzalez-Esquinca A. R., Castro-Moreno M., de-la-Cruz-Chacon I.
Journal of Plant Protection Research
|
2018
|
tom 58
|
nr 3
Several species of Solanum produce secondary metabolites with antimicrobial activity. In the present study, the inhibitory activity of Solanum chrysotrichum, S. erianthum, S. torvum and S. rostratum against phytopathogenic Curvularia lunata was determined. Methanol extracts from roots, stems, leaves and fruits were evaluated by the method of mycelial inhibition on agar and the minimum inhibitory concentration (MIC) was determined on a liquid medium. To increase the antimicrobial activity, the combined activity of the most active extracts for each phytopathogen was also determined (a combination of intra and interspecies extracts). The results showed that 12 of the 16 methanolic extracts of Solanum species had antifungal effects against C. lunata. The extracts of S. rostratum and S. erianthum developed the highest activity (~80% inhibition and 28.4 MIC μg . ml–1), even, equal to or greater than, the reference fungicide. The mixture of the active extracts of S. chrysotrichum and S. torvum increased their activity. Various extracts affected the macro and microscopic morphology and most of them reduced the number of conidia of the fungus. This resulted in the capacity to control the vegetative growth and reproduction of C. lunata, the causal fungus of corn leaf spot disease.
6
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Communities of fungi and bacteria in the rhizosphere of potato and their effect on phytopathogens

84%
Pieta D., Patkowska E., Pastucha A.
Journal of Plant Protection Research
|
2001
|
tom 41
|
nr 2
The purpose of the studies carried out in the years 1996-1998 was to establish the composition of bacteria and fungi communities in the potato rhizosphere and non-rhizosphere soil. Besides, in the examined samples the studies established the proportion of bacteria and fungi antagonistic towards soilborne pathogens. The microbiological analysis of 1 g of dry weight of soil coming from the rhizosphere of potato revealed from 3.96 x 10⁶ to 7.26 x 10⁶ bacteria colonies and from 51.38 x 10³ to 69.96 x 10³ fungi colonies. In the case of nonrhizosphere soil of 1 g of dry weight of soil revealed from 3.50 x 10⁶ to 4.75 x 10⁶ bacteria colonies and from 16.16 x 10³ to 34.10 x 10³ fungi colonies. Moreover, potato cultivation had a positive effect on the increase of numbers of antagonistic bacteria (Bacillus spp. and Pseudomonas spp.) and fungi (Gliocladium spp., Penicillium spp., Trichoderma spp.). A larger number of the communities of bacteria and fungi, including antagonistic ones, in the root area of potato, indicates considerable biological activity, which contributes to a better phytosanitary condition of the soil.
7

Investigation of antibacterial metabolites produced by Streptomyces flavescens B-2223

84%
Kolomiets E. I., Zdor N. A., Romanovskaya T. V.
Bulletin of the Polish Academy of Sciences. Biological Sciences
|
2003
|
tom 51
|
nr 2
8

Biological fundamentals and practical aspects of Trichoderma applications in plant protection against diseases

84%
Pristchepa L. I., Voitka D. V.
Bulletin of the Polish Academy of Sciences. Biological Sciences
|
2001
|
tom 49
|
nr 4
9

Plant-microbe-interactions - a unique type of the stress

84%
Hennig J.
Biological Letters
|
2005
|
tom 42
|
nr 2 Spec.Vol.
10
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Formation of bacterial and fungal communities in the rhizosphere of soybean [Glycine max. [L.] Merrill] and their antagonism towards phytopathogens

84%
Patkowska E.
Journal of Plant Protection Research
|
2001
|
tom 41
|
nr 2
The purpose of the studies conducted in the years 1996-1998 was to determine the quantitative and qualitative composition of bacterial and fungal communities in rhizosphere of soybean cultivated in monoculture and non-rhizosphere soil. Besides, the proportion of bacteria and fungi, which were distinguished by their antagonistic effect towards soil-borne pathogens was established. A microbiological analysis of 1g of dry weight of soil from rhizosphere of soybean resulted in 3.21 x 10⁶ to 8.70 x 10⁶ bacterial colonies and from 70.51 x 10³ to 123.74 x 10³ fungal colonies. In the case of non-rhizosphere soil, 3.50 x 10⁶ to 4.75 x 10⁶ bacterial colonies and 16.16 x 10³ to 51.38 x 10³ fungal colonies were obtained. Besides, soybean cultivation in monoculture had a negative effect on the number of antagonistic isolates of bacteria (Bacillus spp., Pseudomonas spp.) and fungi (Gliocladium spp., Penicillium spp., Trichoderma spp.). Smaller numbers of antagonistic bacteria and fungi in rhizosphere soil of soybean cultivated in monoculture as compared to non-rhizosphere soil, can prove little biological activity, which results in a worse phytosanitaty condition of the soil.
11

Induced resistance in tomato plants by IAA against Fusarium oxysporum lycopersici

84%
Sharaf E. F., Farrag A. A.
Polish Journal of Microbiology
|
2004
|
tom 53
|
nr 2
The phytohormone IAA (indol-3-acetic acid) was tested in vitro on growth of tomato wilt pathogen Fusarium oxysporum lycopersici. The hormone reduced spore germination, mycelial dry weight and protein content. Such reduction was matched with the elevation in the hormone concentration. The in vivo application of IAA to soil of the uninoculated plants (controls) improved growth and yielded longer shoot and root, particularly at low concentrations. Moreover, the hormone could prevent completely any chance for disease incidence by soil pathogens. Presence of IAA in soil of inoculated plants not only reduced the infection rate but also increased plant growth, causing that they appeared healthy and normal. Disease suppression in tomato plants, exerted by application of IAA, was achieved through either increasing plant growth, exerting a direct harmful effect on the target pathogen and/or inducing resistance in host tissue. The induced resistance was correlated with induction of certain secondary metabolites which may have a role in increasing tolerance in tomato plants to the pathogen.
12

Impact of Glomus fasciculatum and fluorescent Pseudomonas on growth performance of Vigna radiata [L.] Wilczek challenged with phytopathogens

67%
Basu M. J., Santhaguru K.
Journal of Plant Protection Research
|
2009
|
tom 49
|
nr 2
190-194
Fusarium oxysporum and Rhizoctonia solani are the major soil-borne pathogens causing growth and yield depression. The present study focused on the ability of fluorescent Pseudomonas and Glomus fasciculatum on growth performance of Vigna radiata in pathogen-infested soil. The percent colonization by G. fasciculatum indicated an increase of the presence of fluorescent Pseudomonas and a decrease of the presence of Fusarium oxysporum or Rhizoctonia solani. However, the reduction of colonization induced pathogen in percent was alleviated by fluorescent Pseudomonas. Inoculation with either fluorescent Pseudomonas or G. fasciculatum or both induced a significant increase in root and shoot length, plant vigour index, dry weight and total N and P content in V. radiata as compared to uninoculated control. The impact of inoculation was much pronounced in dual inoculated plants in comparison with those inoculated with either G. fasciculatum or fluorescent Pseudomonas. In contrast, treatment of plants with either F. oxysporum or R. solani decreased the root and shoot length, plant vigour index, dry weight and total N and P content in the test legume. However, in the presence of fluorescent Pseudomonas and G. fasciculatum, the adverse effect on the pathogens on growth of V. radiata was alleviated.
13

Most deleterious pathogens of lupine and soya and their control by antagonistic bacteria strains in Belarus

67%
Kuptsov V. N., Kolomiets E. I., Kuptsov N. S., Mandrik M. N., Evsegneeva N. V.
Zeszyty Problemowe Postępów Nauk Rolniczych
|
2010
|
tom 554
Phytopathogenic fungi and bacteria - causal agents of anthracnose, fusariose, grey mould, alternarial mould in lupine and bacterial blight in soya were isolated and identified. Microorganisms of genera Bacillus, Pseudomonas, Streptomyces with elevated antagonistic activity towards lupine and soya pathogens were selected. It was demonstrated that bacterial antagonists were able to suppress the development of phytopathogens both in vitro on agar nutrient media and on lupine/soya plants upon seed treatment. Selective action of antagonists against pathogenic species was established. Strains Pseudomonas aurantiaca S-l and Streptomyces anulatus M-46 were distinguished by the highest efficiency with respect to anthracnose and fusariose of lupine cultivars while bacteria Bacillus subtilis M-22 and Bacillus subtilis M-1 inhibited spread of lupine grey mould. Streptomycetes and bacilli displayed superior activity in control of soya bacterial blight. Based on the obtained findings biological method of lupine seed disinfection was elaborated and tested in field experiments. Completed investigations proved attractive prospects of biological method to control pathologies of leguminous crops as an ecologically safe alternative to chemical pesticides.
14
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Mycobiota of rape seeds in Romania. II. Evaluation of potential antagonistic fungi isolated from rape seeds against the main pathogens of rape crop

67%
Sesan T.-E.
Acta Mycologica
|
2014
|
tom 49
|
nr 1
In vitro relationships between identified seed- and soil-borne fungi from rape samples have been investigated in order to evaluate their antagonistic ability as potential biocontrol agents. The bioproduct obtained from the Trichoderma viride Pers. (strain Td50) has been tested in vivo against the main phytopathogens of rape: Sclerotinia sclerotiorum (Lib.) de Bary, Botrytis cinerea Pers., Alternaria spp. and Fusarium spp. in greenhouse at the Laboratory of Mycology and Plant Pathology, Biology Faculty, University of Bucharest – Romania and in the field at the Agricultural Experimental Research-Development Station Caracal (AERDS), Olt district. The T. viride (strain Td50) bioproduct formulated as a powder for the seed treatment has been effective in the protection of rape plantlets against the above mentioned phytopathogens.
15
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Antagonistic bacteria and fungi limiting potato infection by soil-borne pathogenic fungi

67%
Pieta D., Patkowska E.
Journal of Plant Protection Research
|
2003
|
tom 43
|
nr 2
16

Inhibitory effect of methyl jasmonate on development of phytopathogen Alternaria alternata [Fr.] Keissl. and its reversal by ethephon and ACC

67%
Kepczynska E., Kepczynski J.
Acta Physiologiae Plantarum
|
2005
|
tom 27
|
nr 4A
Methyl jasmonate (MeJA) was found to reduce spore germination, hyphal and mycelial growth in Alternaria alternata (Fr.) Keissl. The addition of ethephon or 1-aminocyclopropane-1-carboxylic acid (ACC), ethylene precursor, together with MeJA to the culture medium resulted in a promotion of all developmental stages of the fungus; these compounds partially or completely reversed the inhibition due to MeJA depending on the concentrations applied. MeJA alone had no effect on ethylene production by mycelium, but after 6 days of incubation in the presence of ACC, emanation of this gas increased significantly. Ethylene is involved in reversing the inhibition of A. alternata due to MeJA.
17

Streptomyces griseoviridis as the antagonist of plant pathogens

67%
Kolomiets E. I., Zdor N. A., Romanovskaya T. V., Lobanok A. G., Adamovich O. T.
Bulletin of the Polish Academy of Sciences. Biological Sciences
|
1999
|
tom 47
|
nr 2-4
18

Fungicidal activity of extracts from herbal mixes against selected phytopathogens

67%
Stompor-Chrzan E.
Bulletin of the Polish Academy of Sciences. Biological Sciences
|
1999
|
tom 47
|
nr 2-4
19

Rhizospheral strain Bacillus subtilis 12 A and its influence on vegetable pathogens

67%
Kolomiets E. I., Popov F. A., Romanovskaya T. V., Chikileva A. E., Baeva S. V., Lobanok A. G.
Bulletin of the Polish Academy of Sciences. Biological Sciences
|
2002
|
tom 50
|
nr 2
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