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Permanent changes in the surrounding environment cause long-term stress in birds, which, when lasting days or weeks, affects the activity of the immune system and increases susceptibility to diseases, leading to changes in the levels of haematological parameters. The heterophil-to-lymphocyte ratio (H:L-ratio) is generally considered an independent and robust indicator of stress level in birds. This parameter allows in a simple way to evaluate activity of the immune system and individual health state of adult and nestling birds. It also enables assessing a body response to short- and long-term stress induced by, among others, the surrounding environment, social stress, blood parasites or a greater energy expenditure of females during breeding. Under conditions of field work the determination of the H:L- ratio is not difficult because what is only needed to conduct a blood smear test is a drop of blood that can be easily obtained even from birds of a small body mass. Moreover, an increase in the H:L-ratio is observed after about an hour from the moment of catching a bird contrary to other measurements like the determination of a baseline level of corticosterone. In this article available literature that discusses the impact of various factors on the H:L-ratio in the Great Tit as a species of 'fast-paced' life is reviewed. In adult and nestling birds the H:L-ratio is influenced by various factors — ecological and ecophysiological ones. In some cases the same factor, e.g. brood size manipulation or a type of habitat, can significantly influence the level of the discussed stress indicator as well as it may not show any impact at all. While interpreting the H:L-ratio one must take into account an impact of various ecological and ecophysiological factors on health state, such as habitat, phase of the annual cycle, differences between brood attempts, sex, age as well as on relations with other indicators of condition e.g. body mass or total blood haemoglobin concentration.
Nest building effort has received scant attention in the literature although it may involve costs which can be detected as physiological stress. We prolonged nest construction effort in a population of Spanish Pied Flycatchers by removing nests from nest-boxes and forcing females to build a second nest. In comparison with control nests, the experimental females had to work for longer periods and accumulate more nest material, but nest construction rates (g of nest material per day of construction) were not affected. There was a positive association of clutch mass with nest construction rate. To measure physiological stress, we captured females shortly after laying to obtain blood samples for heat-shock protein quantification. Heat-shock proteins quantify stress at cell level. The level of HSP60 in peripheral blood was positively associated with total nest construction rate (including second nests for experimental females), but not with laying date, clutch mass or experimental treatment. A third of the variation in the HSP60 level was explained by the nest construction rate. Fast nest builders are physiologically stressed, suggesting that the nest construction rate may constitute an index of female physiological performance.
Winter survival of cereals and grasses depends mainly on plant resistance to low temperature and to snow mould fungi. To persist winter plants have to be tolerant to different kind of stresses: abiotic such as low temperature, long-term snow and ice cover, freeze-induced plant desiccation or frequent freezing and thawing, and biotic - many species of snow mould fungi. During the cold acclimation, cereals and grasses become more resistant to both stresses: cold and snow mould. Earlier seeded plants with a greater number of crowns are more resistant to snow mould. Infection caused by snow mould induces a complex plant response, including such processes as the synthesis of PR (pathogenesis-related) proteins (chitinase and β-1,3-glucanas), production of active oxygen species (AOS), synthesis of phenolics, phyotalexins, accumulation of callosis and soluble carbohydrates, and a decrease of water potential. In the paper the most common defence mechanisms against snow mould pathogens are discussed.
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