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Twelve biochemical values of the serum (concentrations of sodium, potassium, calcium, glucose, urea nitrogen, creatinine, total protein, and enzyme activities of ALP, CPK, AspAT, A1AT, amylase) were determined for 102 bison Bison bonasus (Linnaeus, 1758) coming from the free-ranging population of the Białowieża Forest and from the breeding enclosure. The animals were culled in the winter periods of 2000/2001 and 2002/2003. In adult males only the activity of A1AT is significantly higher than in adult females. The remaining values do not differ significantly between the sexes in any of the three age groups (I - calves, II - youngsters, III - adults). Also, no significant differences in serum parameters were observed between healthy males and the ones exhibiting symptoms of posthitis/balanoposthitis. It was established that the following changes in the values of serum parameters are age-dependent: a decrease in potassium and glucose concentrations and the enzymatic activity of ALP, and an increase in AspAT activity. The results were compared to the corresponding data from the research carried out 20 years earlier (1980-1984) and it has been discovered that the values of seven parameters are significantly different as opposed to the previous results. The following differences were established: currently the concentrations of potassium and urea nitrogen are higher in all age groups; the concentration of glucose is higher in the groups of calves and youngsters; creatinine concentration is lower in adults; the activity of AspAT has increased in youngsters, only; the activity of A1AT has increased, and of amylase has decreased in all animals examined. The changes in the values of serum parameters were analysed in the context of data obtained from post-mortem examinations and we conclude that those changes reflect the growing number of patho- morphological changes in internal organs of the bison dissected. Changes localised mainly in the liver and lungs could be related to parasitical infestations; others were noted in kidneys and in the prepuce and penis area in the males with posthitis/balanoposthitis symptoms. In the discussion it is suggested that the deterioration of the physical condition of European bison inhabiting the Białowieża Forest may be connected to such environmental and populational factors as: winter supplementary feeding, winter aggregations, and a decline in immunity related to inbreeding.
The influence of sex, body weight, physical condition, age and season on diet choice was investigated by hunting reports and intestinal analyses of 441 lynx Lynx lynx (Linnaeus, 1758) from Norway killed during 1960-1996. Of self-provisioning (> 1 yr) lynx (n = 280), males preyed proportionately more upon cervids (primarily roe deer Capreolus capreolus and semi-domestic reindeer Rarigifer tarandus) compared to small game (mountain hare Lepus timidus and tetraonids) than females did. Only 5.4% of the variation in prey preference towards small game and cervids (p = 0.0002) could be explained by sex. In a logistic regression model, no additive effect of weight or any other parameters was found after sex had been included. We did not find sufficient evidence for body weight (sensil stricto) being related to prey choice, but propose that sexually determined prey segregation in lynx is caused by different ranging behaviour resulting in different encounter rates with different kinds of prey.
Physical condition in a living at high density (120 individuals/1000 ha of forest area) red deer Cervus elaphus Linnaeus, 1758 population was studied by determining: dressed body weight, kidney fat index (KFI) and amount of visceral fat in 628 animals. Antler weights were also considered as condition index of stags. The mean body weight of calves of both sexes was identical, ie 38.0 kg, that of hinds fluctuated from 57.8 to 75.1 kg, stags - from 63.2 to 132.4 kg depending upon age. The KFI value for female and male calves, hinds, and stags at various age amounted respectively to: 1.54, 1 36, 1.60-1.76, and 1.17-2.69. Per cent of individuals with great amount of visceral fat amounted: in female calves - to 39.4, in male calves - 33.3, in hinds - 47.6-66.7, and in stags — 20.0-100.0% depending upon age. Maximum weight of antlers was 4.2 kg. Despite high population density animals indicated generally good physical condition. Except that the condition of calves of both sexes has been maintained at a similar level, what evidenced an inferior condition of males during their growth. Males in contrast to females, reveal serious decline of condition following the rut, during autumn and winter. It was related to stag age and most marked in the animals of the oldest age-classes (no less than by 40%). Factors affecting the condition of red deer in their population from the Słowiński National Park were discussed.
Background. Early maturation of salmon males (Salmo salar L.) affects the reduction of fish physical condition and culture Materials and Methods. Atotal of 145 salmon males belonging to a group of low growth rate specimens that had not smoltified during the first spawning season were sampled from the "Aquamar" Fish Farm (Miastko, Poland). The study was based on light microscopy examination of histological sections and a standard procedure of milt quality evaluation. The gonadal development stage was determined with Billard and Escaffre ′s 9-grade scale modified by Dziewulska. Results. The mean fork length of males was 10.45 cm. Three groups of males were distinguished: non-maturing (stage I); beginning spermatogenesis (inactive substage II); and precocious (stages VI to IX plus maturing males classified as undergoing "attempted spermatogenesis"). The groups contained 72.4, 4.8, and 22.8% of the males examined, respectively. The gonadosomatic index recorded in the three respective groups ranged from 0.010 to 0.164 (mean 0.040); 0.050 -0.155 (0.089); and 0.058 -6.219 (1.358). The gonadosomatic index is not an accurate indicator of gonadal activity. The precocious males semen contained from 6.1 to 23.0 million spermatozoa per mm 3 (13.41 million on the average). Spermatozoa performing progressive movements constituted 80-90%. Results. On the other hand, precocious male can fertilize mature eggs. The aim of this study was to estimate the magnitude of precocious maturation and to evaluate semen characteristics in a group of cultured 1-year-old salmon. Conclusion. Among non-maturing males and males beginning spermatogenesis, precocious individuals were detected, the latter produced semen of good quality.
The aim of ichthyofauna studies conducted in Lake Smolak (northern Poland) in the 2002-2004 period was to evaluate species richness, growth rate, and fish assemblage structure in light of environmental conditions. This initially dystrophic lake, which, in the 1950s, was inhabited by only two fish species – perch, Perca fluviatilis L., and pike, Esox lucius L., was subjected to experimental liming and fertilization (to stimulate eutrophication) and stocking in the 1971-1974 period. Currently, the ichthyofauna of the lake is comprised of ten species belonging to four families. The density of the fish in this lake is not high; the most numerous are roach, Rutilus rutilus (L.) and bream, Abramis brama (L.). Roach was characterized by a rapid growth rate, but that of bream was very slow. The environmental parameters of the lake undoubtedly have a negative impact on the ichthyofauna, especially the gradients of and seasonal variation in the physical and chemical parameters of the water as well as the absence of submerged hydrophytes and the limited occurrence of helophytes.
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The effect of tillage intensity on changes of microbiological activity and content of particulate organic matter in soil under winter wheat duirng 3 years was studied. Microbial response related to the tillage-induced changes in soil determined on the content of biomass C and N, the rate of CO2 evolution, B/F ratio, the activity of dehydrogenases, acid and alkaline phosphatases, soil C/N ratio and microbial biomass C/N ratio confirmed the high sensitivity of soil microbial populations to the tillage system applied. After three year studies, the direct sowing system enhanced the increase of labile fraction of organic matter content in soil. There were no significant changes in the labile fraction quantity observed in soil under conventional tillage. Similar response related to the tillage intensity was observed in particulate organic matter quantities expressed as a percentage of total organic matter in soil. A high correlation coefficients calculated between contents of soil microbial biomass C and N, particulate organic matter and potentiallymineralizable N, and the obtained yields of winter wheat grown on experimental fields indicated on a high importance of biological quality of status of soil for agricultural crop production.
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