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Colletotrichum dematium was isolated from caraway for the first time in Poland in 2005. Isolations of this fungus were repeated in 2006 and 2007. The cultures of fungus were obtained from superficially disinfected leaves, root necks, roots, stems and umbels. The isolates were identified on culture media: PDA and malt agar with addition of pieces of caraway stems and on the base of macro and microscopic structures. Studies on the biotic effect between C. dematium and other species of phyllosphere fungi of caraway showed that the majority of the studied species limited the growth and development of C. dematium, but the size of the limiting effect was different. The species from Trichoderma and Gliocladium genera were the most effective against C. dematium, causing degeneration and lysis of hyphae and precluded the formation of the pathogen's acervuli and conidia. C. dematium in dual culture with E. purpurascens, A. radicina, S. sclerotiorum, B. cinerea and R. solani produced an inhibition zone which indicated its capacity for antibiosis.
The studies were conducted in the years 1998–2000 on soybean, cv. Polan. The purpose of the studies was to conduct microbiological and mycological analyses of the leaves and pods of soybean. Bacteria and fungi were obtained much more often from the diseased soybean parts as compared to the healthy ones. 778 fungi isolates were obtained as a result of the mycological analysis of the leaves and pods of soybean. Fusarium spp. and Phomopsis sojae were most frequently isolated within pathogenic fungi. Their proportion was 17.5% and 7.8% of all the fungi, respectively. On the other hand, the isolated saprophytic fungi included for example the species from the genera of Acremonium, Cladosporium, Gliocladium, Penicillium and Trichoderma as well as Aureobasidium pullulans and Epicoccum purpurascens. Besides, about a four times lower number of antagonistic microorganisms (Bacillus spp., Pseudomonas spp., Gliocladium spp., Penicillium spp., Trichoderma spp.) was isolated from the infected soybean parts in comparison to the number of microorganisms isolated from the healthy parts.
The study material consisted of K 1860 Septoria carvi isolate and single isolates of 18 other fungal species, chosen randomly from our own collection created during the studies of caraway diseases carried out in 2001-2006. Individual, general and summary biotic effects of the various fungi on Septoria carvi were determined by using the biotic series method and PDA (Difco) medium. Our results show that S. carvi is a weak competitor, because its growth was limited by all studied species of fungi representing the phyllosphere of caraway. Trichoderma, Gliocladium and Epicoccum purpurascens were recognized as positive and effective antagonistic fungi in relation to S. carvi. Moreover, the studied fungi that are pathogens of caraway and other plants, i.e. Fusarium spp., Rhizoctonia solani, Colletotrichum spp., Botrytis cinerea, Alternaria spp. and Phoma exigua proved strongly competitive to S. carvi, so these fungi could mask the symptoms of septoriosis at the first stage of the disease.
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During 2012–2014 in southeastern Poland the species Colletotrichum fuscum was isolated from the leaves of oregano (Origanum vulgare L.) showing the symptoms of necrotic, concentrically zoned spots with a lighter center and a slightly raised edge. Morphology of nine randomly chosen isolates from the fungus population and reference isolate CBS obtained from CBS-KNAW Fungal Biodiversity Centre were studied. Each isolate was cultured on PDA medium, at the temperatures 24°C for 14 days. The character of the cultures, the color of the averse and the reverse, the formation of morphological structures of the fungus, i.e. acervuli, conidia, appressoria and chlamydospores were studied. Ultrastructural observations of morphological structures were undertaken using scanning electron microscopy. The presence of setose acervuli, conidia, chlamydospores and appressoria was visible. Moreover, studies on the biotic effect between C. fuscum and other species of phyllosphere fungi of oregano showed that all tested fungi inhibited the growth of C. fuscum, but the size of the limiting effect was different. Fungi from genera Trichoderma and Clonostachys were found out to be the most effective and positive antagonists. Alternaria alternata, Botrytis cinerea and Fusarium spp. – despite the high values of IBE were considered negative antagonists.
Studies were carried out on the microflora of phyllosphere and soil rhizosphere of hulled (Chwat variety) and naked (Akt variety) oats. The material taken for study embraced samples of leaves and soil rhizosphere taken from cultivations differing in extent of nitrogen fertilization. The studies involved determination of total number of aerobic heterotrophic bacteria belonging to the genus Pseudomonas and microscopic hyphal fungi. Qualitative determinations focused on bacteria belonging to the genera Azotobacter and Azospirillum were also made. Our results point to differences in number of microscopic hyphal fungi in the phyllosphere of both varieties of oats, depending on nitrogen fertilization dose. However, there were no significant differences in the number of bacteria of the different genera determined in the phyllosphere and rhizosphere. Strains of oligonitrophilic and diazotrophic bacteria were isolated from samples of the phyllosphere of oats and their N₂-fixing activity was determined by the acetylene reduction method using gas chromatography.
W pracy przeanalizowano zbiorowiska grzybów w fyllosferze różanecznika pod kątem zdrowotności liści. Badania prowadzono w latach 2004-2007 na różanecznikach w Ogrodzie Botanicznym UJ. Badania znekrotyzowanych liści potwierdziły występowanie patogenów powodujących choroby: Botrytis cinerea - szara pleśń, Colletotrichum gleosporioides - antraknoza, Cylindrocladium scoparium - zgnilizna korzeni oraz plamistość liści i kwiatów, Exobasidium vaccinii - powłocznik azaliowy, Phomopsis archeri - zgorzel pędów, Pycnostysanus azaleae - zamieranie pąków, Rhizoctonia solani - rizoktonioza oraz Septoria azalea - septorioza. Na liściach z objawami nekroz bytowały licznie potencjalne patogeny z rodzajów: Altemaria, Cylindrocarpon, Epicoccum, Pestalotia, Fusarium, Phoma oraz mniej liczne saprotrofy z rodzajów: Acremonium, Arthrinium, Aspergillus, Chaetomium, Cladosporium, Coniothyrium, Humicola, Monodyctis, Mortierella, Nigrospora, Penicillium, Rhizopus, Sordaria, Trichothecium. Liście bez symptomów chorobowych zasiedlone były przez saprotrofy z rodzajów: Acremonium, Aspergillus, Cladosporium, Epicoccum, Humicola, Mucor, Penicillium, Rhizopus, Trichoderma oraz przez mało liczebne patogeny. Wyniki analizy mikologicznej wskazały na dużą bioróżnorodność grzybów zasiedlających zimozielone liście różanecznika, potwierdziły ich zdolność do porażania liści i wywoływania na nich różnorakich nekroz.
All morphological parts of sweet flag (calamus), particularly rhizomes are used not only as a herbal material, but also in confectioner and cosmetic industry. Both quantity and quality of the material which may be obtained exclusively from natural stands are determined by the fungi accompanying the plant’s vegetation. Hence, in the years 2005−2006 research was conducted to determine the diversity of mycobiota which induce chlorosis and necrosis of calamus leaves. It was found that phyllospherae of calamus is colonized by 24 taxa. Ramularia aromatica and Ascochyta acori were the two most often isolated monophagous agents of necrosis. Polyphagous facultative parasites included Fusarium sporotrichioides and Epiccocum nigrum, Alternaria alteranta and A. tenuissima. It is also important that there was the discovery of the presence of Athelia rolfsii and Fusarium incarnatum which are polyphagous species causing destruction of the tissue in tropical and subtropical climate. Due to biochemical activity of most of the isolated species and their ability to induce production of toxins, the content of active substances in calamus may be reduced and consequently diminishes its market value.
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