Wyniki wyszukiwania

1

The occurrence of omega-exonuclease in blood cells

100%

Schaefer H. E.

Folia Histochemica et Cytobiologica

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1984

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tom 22

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nr 3-4

2

Hydrolysis cyclic GMP in rat peritoneal macrophages

100%

Witwicka H., Kobialka M., Gorczyca W. A.

Acta Biochimica Polonica

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2002

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tom 49

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nr 4

Intact rat peritoneal macrophages (rPM) treated with 3-isobutyl-1-methylxanthine (IBMX), an inhibitor of phosphodiesterases (PDEs), accumulated more cGMP than untreated cells. A PDE activity toward [3H]cGMP was detected in the soluble and particulate fractions of rPM. The hydrolysis of cGMP was Ca2+ /calmodulin-independent but increased in the presence of cGMP excess. Similar results were obtained when3 [ 3H]cAMP was used as a substrate. The hydrolytic activity towards both nucleotides was inhibited in the presence of IBMX. Therefore, the PDEs of families 2,5,10 and 11 are potential candidates for cGMP hydrolysis in the rPM. They may not only regulate the cGMP level in a feedback-controlled way but also link cGMP-dependent pathways with those regulated by cAMP.

3

Short-term cardiovascular effects of selective phosphodiesterase 3 inhibitor olprinone versus non-selective phosphodiesterase inhibitor aminophylline in a meconium-induced acute lung injury

84%

Mokra D., Tonhajzerova I., Pistekova H., Visnovcova Z., Mokry J., Drgova A., Repcakova M., Kalkovska A.

Journal of Physiology and Pharmacology

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2013

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tom 64

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nr 6

4

Sildenafil, a phosphodiesterase type 5 inhibitor, downregulates osteopontin in human peripheral blood mononuclear cells

84%

Kaleta B., Boguska A.

Archivum Immunologiae et Therapiae Experimentalis

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2017

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tom 65

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nr 4

5

Cyclic GMP metabolism and its role in brain physiology

84%

Domek-Lopacinska K., Strosznajder J. B.

Journal of Physiology and Pharmacology. Supplement

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2005

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tom 56

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nr 2

15-34

Cyclic GMP (cGMP) is synthesized by guanylyl cyclase (GC) in response to nitric oxide (NO) and carbon monoxide (CO) or natiuretic peptides (NPs); atrial, brain and C-type (ANP, BNP and CNP). cGMP is degraded by several cGMP-specific phosphodiesterases (PDEs). Guanylate cyclases (GC) are differentiated into: membrane-bound/particulate (pGC) and cytosolic/soluble (sGC). In recent years evidence has accumulated that NO is the main activator of sGC and NO/cGMP plays important role in glutaminergic, cholinergic and dopaminergic signaling pathways. cGMP in the nervous system is involved in long term potentiation and depression (LTP, LTD) suggesting its participation in learning and memory mechanism. cGMP regulates calcium homeostasis and phototransduction. Its level is regulated by PDEs and their specific inhibitors protect cGMP level in cells and are very important from clinical point of view.

6

Detection of cyclic nucleotide phosphodiesterase mRNA in mouse skeletal muscle tissue and primary cultured myocytes

84%

Gu J. N., Chen W., Yu T. Q., Lu P., Mu X., Xu J. Q.

Journal of Animal and Feed Sciences

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2007

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tom 16

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nr 4

696-705

7

Cyclic nucleotide phosphodiesterases [PDEs] in human osteoblastic cells; The effect of PDE inhibition on cAMP accumulation

84%

Ahlstrom M., Pekkinen M., Huttunen M., Lamberg-Allardt C.

Cellular and Molecular Biology Letters

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2005

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tom 10

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nr 2

8

Metabolism of cyclic GMP in peritoneal macrophages of rat and guinea pig

84%

Kobialka M., Witwicka H., Siednienko J., Gorczyca W. A.

Acta Biochimica Polonica

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2003

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tom 50

|

nr 3

The aim of our studies was to establish which enzymes constitute the "cGMP pathway" in rat and guinea pig peritoneal macrophages (PM). We found that in guinea pig PM synthesis of the nucleotide was significantly enhanced in response to activators of soluble guanylyl cyclase (sGC) and it was only slightly stimulated by specific activators of particulate guanylyl cyclases (pGC). In contrast, rat PM responded strongly to atrial natriuretic peptide (ANP), the activator of pGC type A. The rat cells synthesized about three-fold more cGMP than an equal number of the guinea pig cells. The activity of phosphodiesterases (PDE) hydrolyzing cGMP was apparently regulated by cGMP itself in PM of both species and again it was higher in the rat cells than in those isolated from guinea pig. However, guinea pig PM revealed an activity of Ca2+ /cal- modulin-dependent PDE1, which was absent in the rat cells. Using Western blotting analysis we were unable to detect the presence of cGMP-dependent protein kinase 1 (PKG1) in PM isolated from either species. In summary, our findings indicate that particulate GC-A is the main active form of GC in the rat PM, while in guinea pig macrophages the sGC activity dominates. Since the profiles of the PDE activities in rat and guinea pig PM are also different, we conclude that the mechanisms regulating cGMP metabolism in PM are species-specific. Moreover, our results suggest that targets for cGMP other than PKG1 should be present in PM of both species.

9

Amyloid beta protein affects poly[ADP-ribose] polymerase activity in PC-12 cells in culture

84%

Strosznajder R. P., Banasik M.

Acta Neurobiologiae Experimentalis

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2000

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tom 60

|

nr 2

10

Antioxidant content in black currants from organic and conventional cultivation

67%

Kazimierczak R., Hallmann E., Rusaczonek A., Rembialkowska E.

Electronic Journal of Polish Agricultural Universities. Series Food Science and Technology

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2008

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tom 11

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nr 2

11

Influence of xanthine derivatives on cough and airway reactivity in guinea pigs

67%

Mokry J., Nosalova G., Mokra D.

Journal of Physiology and Pharmacology. Supplement

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2009

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tom 60

|

nr 5

12

Effects of tadalafil (PDE5 inhibitor) and roflumilast (PGE4 inhibitor) on airway reactivity and markers of inflammation in ovalbumin-induced airway hyperresponsiveness in guinea pigs

67%

Mokry J., Urbanova A., Medvedova I., Kertys M., Mikolka P., Kosutova P., Mokra D.

Journal of Physiology and Pharmacology

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2017

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tom 68

|

nr 5

13

The cAMP analogue, dbcAMP, affects rabbit ovarian cell proliferation, apoptosis, release of steroids and response to hormones

67%

Sirotkin A. V., Balazi A., Chrenek P.

Folia Biologica

|

2014

|

tom 62

|

nr 3

Ograniczanie wyników

The occurrence of omega-exonuclease in blood cells

Hydrolysis cyclic GMP in rat peritoneal macrophages

Short-term cardiovascular effects of selective phosphodiesterase 3 inhibitor olprinone versus non-selective phosphodiesterase inhibitor aminophylline in a meconium-induced acute lung injury

Sildenafil, a phosphodiesterase type 5 inhibitor, downregulates osteopontin in human peripheral blood mononuclear cells

Cyclic GMP metabolism and its role in brain physiology

Detection of cyclic nucleotide phosphodiesterase mRNA in mouse skeletal muscle tissue and primary cultured myocytes

Cyclic nucleotide phosphodiesterases [PDEs] in human osteoblastic cells; The effect of PDE inhibition on cAMP accumulation

Metabolism of cyclic GMP in peritoneal macrophages of rat and guinea pig

Amyloid beta protein affects poly[ADP-ribose] polymerase activity in PC-12 cells in culture

Antioxidant content in black currants from organic and conventional cultivation

Influence of xanthine derivatives on cough and airway reactivity in guinea pigs

Effects of tadalafil (PDE5 inhibitor) and roflumilast (PGE4 inhibitor) on airway reactivity and markers of inflammation in ovalbumin-induced airway hyperresponsiveness in guinea pigs

The cAMP analogue, dbcAMP, affects rabbit ovarian cell proliferation, apoptosis, release of steroids and response to hormones