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Myocardial calcium compartmentation and contractile control

100%

Langer G. A.

Journal of Physiology and Pharmacology

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1991

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tom 42

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nr 1

Under the condition of rapid perfusion, the time course of contractile response of single ventricular cells to extracellular calcium (Ca) depletion and repletion identifies „fast” and „slow” cellular Ca pools. ⁴⁵Ca exchange was studied in these cells under the same conditions of on-line rapid perfusion. Four kinetically-defined compartments were distinguished: (1) A „rapid” compartment containing 2.6mmoles Ca/kg dry wt of lanthanum (La) displaceable Ca, t½ < 1 sec.; (2) An „intermediate” compartments) containing 2.1 mmoles, t½ = 3 and 19 sec. Caffeine displaced significant amounts of Ca from this compartment whereas La displaced none; (3) A „slow” compartment containing 1.6 mmoles, t½ = 3.6 min. Addition of inorganic phosphate to the perfusate adds significant amounts of Ca to this compartment; (4) An „inexchangeable” compartment, containing 1.2 mmoles. The „rapid” compartment’s flux is > 300 µmoles Ca/kg wet wt/sec. Its exchange rate indicates that it is the kinetic counterpart of the functionally-defined „fast” pool. Its subcellular locus is undefined. The „intermediate” compartment is best correlated with the „slow” pool and represents Ca in the sarcoplasmic reticulum. The „slow” compartment contains a significant fraction from the mitochondria. The results indicate that > 40% of cellular Ca can turn over within the period of one contraction cycle. These results are consistent with the following sequence: (1) Upon sarcolemmal depolarization, Ca moves through the Ca channel to arrive at the SR and at the myofilaments. (2) Ca induced Ca release occurs via the „feet” at the SR-inner SL region. The Ca diffuses to the myofilaments or is transported across the SL via the Na-Ca exchanger. (3) Ca is pumped into the free or longitudinal SR and diffuses to the cistemae. Ca is pumped across the SL by the SL Ca pump and by the Na-Ca exchanger. (4) Mitochondrial Ca exchange via the Na-Ca exchanger and/or SL Ca pump. (Supported by NHLBI and the Laubisch and Castera Endowments.)

2

Effect of the addition of zinc and selenium ions on the stability of the Biolasol liquid used for perfusion, reperfusion and preservation of parenchymal organs of the abdominal cavity

84%

Ostrozka-Cieslik A., Dolinska B., Caban A., Ryszka F.

Journal of Elementology

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2015

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tom 20

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nr 2

The Biolasol® liquid is an innovative solution used for perfusion, reperfusion and preservation of parenchymal organs of the abdominal cavity. Substances in the liquid prevent cellular oedema and help to maintain a proper water/mineral as well as acid/base balance in the intracellular environment. They also minimize free-radical injuries and ensure the integrity of the cellular membrane structure. The Biolasol® liquid has been shown to be much more efficient than the HTK liquid in the preservation of kidneys. The Biolasol® liquid containing 0.5 mM of vitamin C has been modified by adding ions of Se(IV), Zn(II), and their effect on the stability of the solution was examined. An accelerated aging test was applied to test the liquid stability. The test, based on the laws of chemical kinetics, was conducted at four temperatures at a 10°C step, that is: 50°C±0.05, 60°C±0.05, 70°C±0.05 and 80°C±0.05. The relative humidity equalled 75% of RH and the duartion of the test was 40 days. In order to determine the stability of the tested solutions, the Arrhenius Dependence equation was used, applied to the effect of temperature on the glucose decomposition reaction rate: lnk=lnA-(Ea/RT). The results indicate that the addition of zinc decreases the stability of the liquid by 30.5%, while the addition of selenium prolongs the stability by 8.21%. This is explained by the synergism of action of vitamin C and Se4+ antioxidant in the tested liquid. Zinc ions present in the solution increase the glucose decomposition reaction rate.

3

Exploring possibilities for an alternative approach in experimental schistosomiasis mansoni: the peritoneal cavity of mice

84%

Mati V. L. T., Bicalho R. S., Melo A. L.

Acta Parasitologica

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2017

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tom 62

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nr 1

4

Changing morphology in the human cotyledon subjected to dual perfusion in vitro exposed to variable magnetic fields

84%

Lopucki M., Lancut M., Kankofer M., Rogowska W., Czerny K., Jedrych B., Pietruszewski S., Stachowicz N., Kornarzynski K., Kotarski J.

Polish Journal of Environmental Studies

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2004

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tom 13

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nr Suppl.2, Part 1

The paper presents the results of morphological examinations of the human cotyledons perfused in vitro and exposed to variable magnetic field (MF) during the 180-minute experiment. The cotyledon biopsies were collected immediately after perfusion and morphologically examined using the electron microscope. The control group C (10 perfusions) was not exposed to MF. In the experimental group E, (10 perfusions), the cotyledons were exposed to the 2mT, 50Hz variable magnetic field while in the experimental group E2 (10 perfusions) the 5mT, 50 Hz was used. In the groups E, and E2, numerous indentations of the sheath (areola) in the syntrophoblast nuclei were found, condensed (thickened) nuclear chromatin right beneath the sheath was substantially reduced and pyknosis was observed in some nuclei. The villi revealed widened vascular - epithelial membrane resulting from the oedema of the endothelial cells. Moreover, an increased number of collagen fibres in the villi and decreased number of active mitochondria were observed in the group E2

5

Perfusion culture promotes differentiation of oral keratinocytes in vitro

84%

Hagedorn G. M., Blank A., Mai R., Weiland B., Spassov A., Lauer G.

Journal of Physiology and Pharmacology. Supplement

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2009

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tom 60

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nr 8

25-29

6

Influence of a rat kidney perfusion using the anti-MHC class II monoclonal antibody and reduced dose of 15-deoxyspergualine on the allogeneic graft survival

84%

Oko A., Krzymanski M., Deja A., Ulrichs K., Waaga A. M., Muller-Ruchholtz W.

Archivum Immunologiae et Therapiae Experimentalis

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1995

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tom 43

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nr 5-6

7

Influence of endogenous and exogenous histamine on the placental vascular resistance in diabetes complicated pregnancy

84%

Szukiewicz D., Szukiewicz A., Maslinska D., Gujski M., Poppe P., Markowski M.

Journal of Physiology and Pharmacology. Supplement

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1997

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tom 48

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nr 2

8

Effect of galanin on substance P- and vasoactive intestinal polypeptide-induced nociceptive trigemino-hypoglossal reflex in rats

84%

Zubrzycka M., Janecka A.

Journal of Physiology and Pharmacology

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2007

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tom 58

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nr 3

Substance P (SP), vasoactive intestinal polypeptide (VIP) and galanin (GAL), present in primary sensory neurons, are involved in transmission of nociceptive signaling from the peripheral to central nervous system. In this study we investigated the effect of GAL on SP-induced or VIP-induced evoked tongue jerks (ETJ) in response to noxious tooth pulp stimulation during perfusion of the cerebral ventricles with SP or VIP solutions. The experiments were carried out on rats under chloralose anesthesia. It was shown that both, SP and VIP, perfused through the cerebral ventricles enhanced the ETJ amplitude as compared with control, but the effect produced by SP was stronger. The intracerebroventricular perfusion of GAL 5 minutes before SP caused a dose-dependent inhibition of SP-induced ETJ, whereas GAL perfused through the cerebral ventricles 5 minutes before VIP did not reduce the excitatory effect of VIP on ETJ. These results indicate that the antinociceptive effect of GAL perfused through the cerebral ventricles, tested on the trigemino-hypoglossal reflex in rats, is specifically mediated by the SP-ergic system.

9

Influence of dopamine on vascular resistance - human placenta perfusion study

84%

Szukiewicz D., Szukiewicz A., Maslinska D., Poppe P., Gujski M., Piorkowski R.

Journal of Physiology and Pharmacology. Supplement

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1997

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tom 48

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nr 2

10

Effect of cerebral ventricles perfusion with morphiceptin and met-enkephalin on trigemino-hypoglossal reflex in rats

84%

Zubrzycka M., Fichna J., Janecka A.

Journal of Physiology and Pharmacology

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2002

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tom 53

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nr 4,2

Opioids administered by intracerebroventricular injections produce analgesic responses in rats. The present study was undertaken to investigate the effects of a highly selective m-opioid receptor ligand morphiceptin on trigemino-hypoglossal reflex in rats. The analgesic effect of morphiceptin was compared with another opioid peptide, Met-enkephalin. With the experimental settings used in this study, we have demonstrated that both morphiceptin and Met-enkephalin show significant dose-dependent analgesic effects after i.c.v. administration in rats as assayed by trigemino-hypoglossal reflex test. The antinociceptive response to Met-enkephalin was short lasting and was observed 10 to 15 min after i.c.v. perfusion. Morphiceptin had a relatively longer duration of antinociceptive action, the effect was observed 20- 50 min after i.c.v. perfusion. Neither morphiceptin nor Met-enkephalin produced antinociception after peripheral injections. The results of the present study indicate that both tested peptides act at µ-opioid receptors situated in the central nervous system. They also suggest that µ-opioid receptors present in the central nervous system are an important element of the trigemino-hypoglossal reflex arc. For that reason selective m-opioid receptor ligands, like morphiceptin, inhibit the reflex more significantly.

11

Alterations of tumor and normal tissue of human lung cancer resection specimens after isolation perfusion

67%

Nowak K., Hanusch C., Kolbel H. C., Schwarzbach M., Post S., Beck G., Gebhard M. M., Metzger R., Hohenberger P.

Journal of Physiology and Pharmacology. Supplement

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2007

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tom 58

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nr 5

12

The influence of the magnetic field on the glucose consumption and lactic acid production in the in vitro perfused human placental cotyledon

67%

Lopucki M., Kankofer M., Pietruszewski S., Kornarzynski K., Rogowska W., Stachowicz N., Semczuk-Sikora A., Czekierdowski A., Kotarski J.

Polish Journal of Environmental Studies

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2004

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tom 13

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nr Suppl.2, Part 1

Objective: The biological effects of variable magnetic fields (MF) generated by widely used electrical devices on living organisms are not well understood. However MF may be potentially hazardous for pregnant women, this problem was not profoundly studied. The aim of this study was to evaluate the influence of the variable magnetic field (MF) on the placental metabolism by measurement of glucose consumption and lactate production in the human placenta under dual recirculating perfusion conditions. Material and methods: Altogether 20 term human placental cotyledons were exposed to homogeneous variable MF of B = 2 mT and f = 50 Hz (n=10; group E,) or of B = 5 mT and f = 50 Hz (n=10; group E2) during 3 hours dual close perfusion in vitro. 10 term placental cotyledons were perfused without an exposure to the MF and they served as a control group. Results: The results showed no effect of used MF on the glucose consumption either in group E, or in group E2in. There was also no influence of MF on lactic acid production in group Er In group E2, however, a significant increase of lactic acid production in the fetal circulation from 90lh to 180th minute and in the maternal circulation at 180 minute was noted. Conclusions: An increase in the lactic acid production in the group E2in observed during the experiment may result from hindered oxygen supply to the placenta and intensified anaerobic metabolism of glucose.

Ograniczanie wyników

Myocardial calcium compartmentation and contractile control

Effect of the addition of zinc and selenium ions on the stability of the Biolasol liquid used for perfusion, reperfusion and preservation of parenchymal organs of the abdominal cavity

Exploring possibilities for an alternative approach in experimental schistosomiasis mansoni: the peritoneal cavity of mice

Changing morphology in the human cotyledon subjected to dual perfusion in vitro exposed to variable magnetic fields

Perfusion culture promotes differentiation of oral keratinocytes in vitro

Influence of a rat kidney perfusion using the anti-MHC class II monoclonal antibody and reduced dose of 15-deoxyspergualine on the allogeneic graft survival

Influence of endogenous and exogenous histamine on the placental vascular resistance in diabetes complicated pregnancy

Effect of galanin on substance P- and vasoactive intestinal polypeptide-induced nociceptive trigemino-hypoglossal reflex in rats

Influence of dopamine on vascular resistance - human placenta perfusion study

Effect of cerebral ventricles perfusion with morphiceptin and met-enkephalin on trigemino-hypoglossal reflex in rats

Alterations of tumor and normal tissue of human lung cancer resection specimens after isolation perfusion

The influence of the magnetic field on the glucose consumption and lactic acid production in the in vitro perfused human placental cotyledon