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Tests were done on the model station situated on the territory of Szczecin–Pomorzany Water Treatment Plant. The tested water came from Kurowski Channel. Kurów is the river bank water intake from Kurowski Channel – an artificial arm of Western Odra River. Pumping station is situated 300 m far from the Kurowski Channel. Water is supplied to the intake chamber of the pumps using the pipeline 1400. Three pumps of Flyght CP 3305 type in the alternating system force the raw water through two pipelines 700 PE to Pomorzany Water Treatment Plant situated 3 km far from it. Kurowski Channel is supplied from the main stream of Odra River and the inflowing underground waters of Wysoczyzna. Average composition of the water coming from Kurowski Channel is much more better than the water taken directly from Odra River.
The aim of the present study was to evaluate the effect of fish size (BW 11.7 g in the PS group, and 28.1 g in the PL group), feeding (SDA effect), and starvation (4, 13, 19 or 26 days in the PS-S group, and 4 or 13 days in the PL-S group) on oxygen consumption (OC, mg O₂ kg⁻¹ h⁻¹), and ammonia excretion (AE, mg TAN kg⁻¹ h⁻¹) of the juvenile pike-perch Stizostedion lucioperca (L.) reared in water recirculation systems. The fish were fed high-protein commercial trout pellets for 18 h d⁻¹ at daily rates of 2.5% (PS) or 1.2% (PL) of stock biomass. The average OC and AE values of the fed pike-perch from the PS group were equal to 355.70 mg O₂ kg⁻¹ h⁻¹ and 21.21 mg TAN kg⁻¹ h⁻¹, respectively, and were 35% (OC) and 62% (AE) higher than in the PL group (P<0.05). The metabolic rate of the starved fish was also inversely related to fish size and showed significant differences (P < 0.05). The energetic value of SDA was 62.8 kJ kg fish d (7.3% offeed digestible energycontent) in the PS group, and 31.6 kJ kg fish d (5.2% of digestible energy) in the PL group. The starvation period of four days was too short for OC and AE stabilization. The values of both parameters stabilized in the second and third week of starvation.
The oxygen consumption of a sandy beach interstitial community was determined on four occasions (January, May, August, October) on the Gulf of Gdańsk (southern Baltic Sea). The study was carried out at four locations on the beach slope (littoral, waterline, splash zone and middle beach). Oxygen consumption varied from 158–159 cm3 O2 m−2 h−1 at the underwater site and waterline to 20–36 cm3 O2 m−2 h−1 in the middle beach. According to these data, interstitial organisms are able to utilize from 206 to 1641 mg of organic carbon per square metre per day. In general, metabolic activity decreased gradually from the waterline towards the middle beach, and a significant correlation was found between oxygen consumption and sediment water content. Changes in oxygen consumption on the beach slope were statistically significant.
This study used a novel in vivo model to test the hypothesis that nutritive and non-nutritive blood flow distribution can still be observed under conditions of high vascular tone and oxygen delivery at rest and in metabolically active (twitch contracting) skeletal muscle. Experiments were performed in a constant flow autologous pump-perfused hindlimb in anaesthetised male Wistar rats. Agonists were tested at rest with a flow rate of 1ml•min-1, and during hindlimb muscle twitch contractions (sciatic nerve stimulation: 6V, 1Hz, 0.05ms, 3min) at a flow rate of 2ml•min-1. Oxygen consumption was determined from hindlimb venous and arterial blood samples. Resting perfusion pressure at 1ml•min-1 was 92±3 mmHg (N=15) and oxygen consumption was 0.41±0.05 µmol•min-1•g-1. Serotonin increased perfusion pressure and significantly decreased basal hindlimb oxygen consumption at rest. During acute muscle contraction this effect on oxygen consumption was diminished. Noradrenaline significantly increased perfusion pressure but had no significant effect on basal hindlimb oxygen consumption. Vasoconstriction that impacts upon muscle metabolism occurs in vivo, which potentially could be due to selective redistribution of blood flow. However, during muscle contraction local release of vasodilatory regulation can overcome exogenously-induced vasoconstriction. These results support the hypothesis that dual vascular pathways may explain differential vasoconstriction and how it impacts upon muscle metabolism.
The aim of the study was to determine the oxygen consumption (OC, mg O₂ kg⁻¹h⁻¹) and ammonia production (AE, mg TAN kg⁻¹h⁻¹) of perch reared in a recirculating system at the optimal temperature for this species of 23°C. The studies were conducted when the fish were undergoing intense fattening on artificial feed. Measurements of metabolic rate were taken 24 hours per day in six size groups of fish (mean body weights (BW) in each group - 18.4, 30.7, 46.4, 56.5, 67.8, 82.3 g). Within the studied perch size range, OC decreased from 336.2 to 185.0 mg O₂ kg⁻¹h⁻¹, and AE from 22.0 to 5.6 mg TAN kg⁻¹h⁻¹. An increase in body weight by 1 g led to an average decrease in oxygen consumption by a mean of 2.53 mg O₂ kg⁻¹h⁻¹ and ammonia excretion by 0.28 mg TAN kg⁻¹ h⁻¹. During the analyzed period, the body weight of perch, the arithmetic dependence between OC-BW and AE-BW, was linear and the determination coefficients R² of the linear regression equations describing these relationships were highly statistically significant at a value exceeding 0.9.
The aim of the study was to determine what impact feeding juvenile common whitefish, Coregonus lavaretus (L.), different feed rations had on rearing indexes and oxygen consumption during culture in recirculation systems. Three feeding treatments were applied: 2% (group L), 3% (group M), 4% (group H) of fish biomass. The final juvenile common whitefish weight increased with increasing daily feed rations. Fish from group H (largest feed ration) had statistically significantly (P < 0.05) higher body weights (50.3 g) and higher feed conversion ratios (1.14) than did the fish in the other two groups. However, the lowest oxygen consumption (192.9 mg O2 kg-1 h-1) was noted in group L (smallest feed ration). The results of the experiment indicated that the size of the daily feed ration has a significant impact on the rearing results and oxygen requirements of juvenile common whitefish. The optimum daily feed ration for juvenile whitefish weighing 10-45 g and at a water temperature of 18.0°C is 3% of the stocking density.
Anaesthetics are needed when handling fish, especially during tagging. However, most anaesthetics applied at present have a strong toxic effect on fish. For this reason it is only permissible to keep fish anaesthetized for a short time. A new anaesthetic, Propiscin, which allows fish to be anaesthetized for up to 0.5 h, has been successfully tested. It contains a 0.2% stabilized solution of etomidate and can be used as a bath. When administered correctly, the required disappearance of sense perception and motor reflexes in the fish can be obtained in about 2 - 4 min. The low toxicity of this pharmacological confection has been proved according to a full set of clinical, toxicological, hematological and biochemical criteria. Clinical tests have been conducted on with many fish species, mainly salmonids.
This study investigated the impact of water temperature on the growth, feed uptake, and oxygen consumption of juvenile whitefish, Coregonus lavaretus (L.), reared in a recirculating system. The fish used in the study had a mean initial weight of 7.9 ± 0.2 g and a mean body length of 8.9 ±0.1 cm. The study was conducted at water temperatures of 20, 22, and 24°C (each group in replicates of four). The best weight gain and body length growth were obtained at the water temperature of 22°C. Increasing the temperature to 24°C caused a statistically significant decrease in fish growth rate (P < 0.05). The feed conversion ratios of the fish reared at water temperatures of 20 and 22°C were close at 0.89 and 0.90, respectively, while at the temperature of 24°C this ratio was statistically significantly higher at 1.17 (P < 0.05). Temperature was not found to have a statistically significant impact on fish survival or on the amount of feed consumed. Water temperature had an impact on oxygen consumption, and the mean value of this index at a temperature of 20°C (186.6 mg O₂ kg⁻¹ h⁻¹ ) was significantly statistically lower (P < 0.001) than in the water at temperatures of 22 and 24°C, at which the mean values were 349.3 and 409.9 mg O₂ kg⁻¹ h⁻¹, respectively. Based on the results obtained, it was confirmed that the upper thermal threshold during the rearing of whitefish juveniles is 22°C.
Tachykinins (TK) are family of peptides including substance P (SP), substance K (SK) and neuromedin K (NK) that have been found in the nerves of the gastrointestinal tract and proposed to act as neurotransmitters to affect the motor, secretory and circulatory functions of the gut, but little is known about their action on the pancreas. In this study three series of tests were carried out to determine the action of SP, SK and NK on pancreatic secretion in conScious dogs and amylase release from the dispersed rat pancreatic acini and to correlate the alterations in pancreatic secretory and circulatory effects of TK in anesthetized dogs. SP, SK and NK infused i. v. in graded doses (0.12-1.0 µg/kg per h) in conscious dogs stimulated pancreatic protein outputs reaching, respectively, 38% and 23% of the maximal response to CCK (40 pmol/kg per h). HCO₃ outputs were also significantly increased but the highest response did not exceed about 5% of secretin (328 pmol/kg per h) maximum. Cholinergic blockade by atropine abolished the pancreatic responses to tachykinins. When added at various concentrations (10⁻¹¹ -10⁻⁷ M) to the incubation medium of rat dispersed pancreatic acini, SK, SP and NK increased in concentration-dependent manner the release of amylase from the resting pancreatic acini and augmented the enzyme release induced by CCK-8 and by urecholine. In anesthetized dogs infused with a background dose of secretin (82 pmol/kg per h), addition of SP, SK and NK caused an immediate and dose-dependent increase in the pancreatic blood flow, oxygen consumption and pancreatic secretion accompanied by a dose-dependent decrease in arterial blood pressure. This study shows that TK are potent pancreatic circulatory stimulants and moderate secretagogues both in vivo and in vitro, acting, at least in part, via cholinergic pathway.
Evidence indicates that nitric oxide (NO) suppresses myocardial oxygen consumption (MVO2) and regulates myocardial substrate oxidation, however data from in vivo and isolated heart preparations are conflicting. In addition, cardiac endothelin (ET-1) release has been shown to increase with inhibition of NO synthase (NOS), however the effects of ET-1 on myocardial energetics is not clear. We employed the isolated rat heart model to assess the role of NO and ET-1 on myocardial function and metabolism. Oxidation of glucose and FFA was measured using [U-14C]glucose and [9,10-3H]palmitate. NOS inhibition with NG-methyl-L-arginine acetate salt (L-NMMA, 50 µM), resulted in an increase in MVO2 at a given rate of myocardial external workload, and no change in myocardial glucose or FFA oxidation. ET-1 (25 pM), which caused coronary vasoconstriction similar to that produced by L-NMMA, also increased MVO2 without an effect on cardiac workload, or substrate oxidation, suggesting a role for ET-I in the regulation of myocardial energetics. We assessed also the effect of ETA/ETB receptor blockade (tezosentan; 5 nM) on MVO2 and glucose and FFA oxidation and observed no effect, suggesting that basal ET-1 production does not play a role in regulating MVO2 or substrate selection. In conclusion, inhibition of NOS or the addition of ET-1 resulted in an increase in MVO2, but did not affect glucose or FFA oxidation.
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