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The objective of this study was to compare the efficacy of human chorionic gonadotropin (hCG) for the stimulation of ovulation within 48 h after treatment in mares, which had a ≥35 mm follicle. A total of 41 oestrus cycles of 21 mares (9 Thoroughbred, 6 Dutch Warmblood, and 6 Friesian), between 7 and 12 years of age were used for this study. All the mares were intramuscularly treated with Hcg, at the dose of 3 000 IU when a follicle ≥35 mm was detected. They were examined daily by transrectal ultrasonography, until the ovulation was confirmed. It was found that the diameter of follicle before ovulation was more significant, and oestrus duration was longer in Friesian mares than those of Thoroughbred and Dutch Warmblood ones. Moreover, the percentage of mares ovulating within 48 h after hCG treatment was significantly lower in Friesian mares, when compared to that of the other breed mares. There was no significant difference in these parameters between Thoroughbred and Dutch Warmblood mares. In conclusion, the efficacy of hCG may be different among mares of different breeds. The percentage of ovulation within 48 h after hCG treatment was lower in Friesian mares when compared to the other breeds. Therefore, various strategies are needed for the stimulation of ovulation in Friesian mares.
Substance P (SP) infused into the third cerebral ventricle blocks spontaneous ovulation in female rats, probably through catecholaminergic neurons. The studies presented in this paper were undertaken to investigate whether SP exerts its suppressing effect on ovulation in 6-hydroxydopamine (6-OHDA) pretreated female rats. After 8-12 days following 6-OHDA pretreatment female rats were infused, on the day of proestrus, with vehicle and all animals were found to ovulate or with a solution containing 5 nmol of SP and 89% of female rats were then found to ovulate. In the group pretreated with vehicle and subsequently infused with SP, ovulation was found to occurr only in 25% of animals. The obtained results indicate that spontaneous ovulation in 6-OHDA-pretreated female rats cannot be blocked by i. c. v. administration of SP, and it may be concluded that SP exerts its suppressing effect through the monoaminergic neurons.
On the day of ovulation and next on day 7 after ovulation the ovaries of 20 sheep were examined using the transrectal ultrasound method. To verify the results, similar examination was made with the laparoscopic method. Depending on the stage of the sexual cycle (day 1 vs day 7) the number of Graafian follicles (∅ ≥5 mm) was determined as well as the total number of corpora lutea found in both ovaries. Out of the total number of 53 Graafian follicles found laparoscopically, 42 (79,2%) were detected with the ultrasound method. The detectability of corpora lutea with the ultrasound method was found similar (79,5%). A considerable improvement in the accuracy of ultrasonic measuring was observed when the determinations were repeated next year. This reflects the effect of the experience gathered by the person taking the measurements.
Multiple ovulations and resulting multiple pregnancies leading to abortions or births of underdeveloped foals are important problems in Thoroughbred reproduction. The incidence of multiple ovulation during one ovarian cycle per mare in 44 Thoroughbred mares was determined by ultrasonic scanning. Twenty-two mares (50%) ovulated two (27.3%) or three (22.7%) dominant follicles during observed follicular phase. Overall 76 dominant follicles were ovulated, including 45 (59.2%) from right and 31 (40.8%) from left ovary. The mares at the age 6-10 years tended to have higher multiple ovulation rate (63.2°/0) than those below 6 or of more than 10 years old (both age groups amounting to 40%). Multiple ovulation rate was found lower in lactating than in non-lactating mares (-16.7 and 51.7%, respectively). No significant differences in the incidence of single vs multiple ovulation were found between the left and right ovary, age, or reproductive status of mares. The pregnancy on day 17 occurred in 7 (15.9%) single-ovulating mares. Twelve multiple-ovulating mares (27.3%) were diagnosed to be pregnant, including 7 (15%) with single and 5 (11.4%) with twin pregnancy. The pregnancy rate did not significantly differ between single- and multiple-ovulating mares.
The aim of this study was to investigate superovulatory responses of cows according to the status of various follicular classes on day 0 (day of initiation of the superovulatory treatment). One hundred and two superovulation cycles were selected. The animals were superstimulated between days 8 and 12 of oestrous cycle with 400 mg of Folltropin-v given in decreasing doses over a 4 d period and luteolysis was induced with two intramuscular injections of cloprostenol. The ovaries of all cows were examined by ultrasonography on day 0, at oestrus, and on day 7 after oestrus. The results showed that the follicular and ovulatory responses were higher (P<0.0l ) in cows that were superovulated in the presence of a high (≥6) number of follicles than in the presence of a low (≤5) number of follicles 4 to 6 mm in diameter. Follicular and ovulatory responses did not differ according to the status of the three other follicular classes studied. Additionally, the mean numbers of fertilised structures and unfertilised ova were not influenced by the status of any of the follicular classes studied. In conclusion, the follicular and ovulatory responses were influenced by the status at initiation day of the superovulatorv treatment.
Progesterone (P4), which is produced by the corpus luteum (CL), creates proper conditions for the embryo implantation, its development, and ensures proper conditions for the duration of pregnancy. Besides the non-genomic activity of P4 on target cells, its main physiological effect is caused through genomic action by the progesterone nuclear receptor (PGR). This nuclear progesterone receptor occurs in two specific isoforms, PGRA and PGRB. PGRA isoform acts as an inhibitor of transcriptional action of PGRB. The inactive receptor is connected with chaperone proteins and attachment of P4 causes disconnection of chaperones and unveiling of DNA binding domain (DBD). After receptor dimerization in the cells’ nucleus and interaction with hormone response element (HRE), the receptor coactivators are connected and transcription is initiated. The ratio of these isoforms changes during the estrous cycle and reflects the different levels of P4 effect on the reproductive system. Both isoforms, PGRA and PGRB, also show a different response to the P4 receptor antagonist activity. Connection of the antagonist to PGRA can block PGRB, but acting through the PGRB isoform, P4 receptor antagonist may undergo conversion to a strongly receptor agonist. A third isoform, PGRC, has also been revealed. This isoform is the shortest and does not have transcriptional activity. Alternative splicing and insertion of additional exons may lead to the formation of different PGR isoforms. This paper summarizes the available data on the progesterone receptor isoforms and its regulatory action within the female reproductive system.
Effects on the reproduction of C. gariepinus after ovulation stimulation with carp pituitary homogenate (CPH), Ovopel or Dagin were investigated. After the application of Ovopel all the females spawned while after CPH or Dagin treatment spawning was reduced to 83.3%. No statistically significant effect of the stimulator was found on the weight of eggs expressed in g and in % of female B.W. however, the highest mean values of these parameters were found after Dagin. A statistically significant effect of the stimulator was noted for the percentage of fertilization and living embryos after 24h and 28h incubation. The highest percentage of live embryos after a 28h incubation of eggs was found after the treatment with Dagin and the lowest after Ovopel; the difference between the means of these stimulators was statistically significant. The females ovulated 12h after Ovopel treatment yielded eggs of a higher weight and of significantly higher quality compared with those obtained 3h later. The relative effectiveness of reproduction, expressed as the number of live embryos per kg of female B.W., was highest after Dagin treatment.
A relatively high rate of multiple ovulation and resulting multiple pregnancies are one of main problems in Thoroughbred reproduction. The aim of the study was to examine the relationship between the intensity of oestrous behaviour and incidence of multiple (MO) vs single (SO) ovulations in 47 Thoroughbred mares. The frequency of five distinct receptive and 12 non-receptive behavioural traits was analysed. For quantitative analysis of sexual behaviour an 8-point behavioural score (BS) expressing increasing mare’s sexual receptivity was used. MO occurred in 48.9% of ovarian cycles observed. Neither frequencies of receptive behaviours, nor BS differed significantly between MO and SO mares. Overall pregnancy rate per one ovulatory cycle was estimated as 40.4%. Pregnancy rate was higher in MO mares (52.2%) as compared to SO mares (29.2%); however, the difference was not proved significant.
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