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Oviposition behaviour is recognized as an ecologically significant trait in insects. Since herbivorous insect females have a potential to lay eggs on unsuitable host species, it is hypothesized that this behaviour may be the first step in host-range expansion. The most intriguing phenomenon, found in some insect species, is the ability of females to lay eggs on unsuitable places if hosts are unavailable; this behaviour is termed ‘egg-dumping’. Egg-dumping can be adaptive plastic response in environments where hosts, either preferred or less suitable, may eventually become available. Transgenerational effects of this behaviour presume changes in larval feeding experience on different host species. Also, diverse larval experience may induce alterations in oviposition behaviour. To test whether egg laying behaviour of adult females can be modified by larval experience, we performed the experiment on two laboratory populations of Acanthoscelides obtectus (Say). First population (P) is housed in the laboratory on the common bean for 200 generations, whereas the second population (C) is derived from the first but has been raised for 197 generations on chickpea, a novel and less suitable host for larval development. In order to analyze differences between short-term and long-term effects of larval experience on eggdumping, both populations have been exposed to each host seed for one generation. The results of the study show that females from P population lay eggs more frequently in the absence of seeds when developed on chickpeas, compared with P females reared on beans. By contrast to P females, in C population, larval experience has no significant effects either on the number of dumped eggs or on the frequency of dumper females. These results demonstrate a significant inter-population diversification of phenotypic plasticity in egg-dumping behaviour depending on larval host experience. In other words, modification of oviposition behaviour, as a consequence of a long-term larval nutritional stress, can evoke previously unrecognized transgenerational effects.
In a population of Rhode Island White hens heritability of egg formation, clutch characters and traditional selection traits as well as the genetic and phenotypic correlations between them were estimated via multitrait animal model. Over 1300 birds and about 4000 birds were recorded in two consecutive generations for oviposition time and traditional traits, respectively. The heritability estimates obtained for age at first egg (h2=0.42), egg weight (h2=0.50) and body weight (h2=0.42) were considerably higher than those for initial egg production (h2=0.22), clutch traits (h2 between 0.11 and 0.23) and oviposition time (h2 between 0.13 and 0.19). Both genetic and phenotypic correlations between clutch traits and traditional selection traits were low, except for initial egg production and maximal clutch length (rg=0.40 and rp=0.38). As expected, negative correlations were registered for number of clutches and average clutch length. It indicates an opportunity of selection aimed at improvement of egg production persistence by an increase in the average clutch size. Oviposition time was favourably correlated with traditional selection criteria.
Studies on rearing the beetles of the Western corn rootworm (WCR) (Diabrotica virgifera Le Conte) under laboratory and field conditions were carried out in 2008–2009. Obtained results allowed for the identification of the reproductive potential and longevity of the females of this species under different rearing conditions. The method used for rearing beetles may be used for further studies on the development and control of larvae under controlled conditions.
In emus, the duration of the fertile period was measured following a single artificial insemination (AI) and investigated the effect of time of AI in the egg cycle on the duration of the fertile period. Semen was collected by artificial cloaca, pooled and used undiluted for AI within 30 minutes. For insemination, a female was followed until she assumed the voluntary crouch. A speculum was then inserted into the cloaca and an insemination straw introduced into the vagina to a depth of 1-2 cm, and semen deposited. Following a single insemination with 100, 200 or 400 million spermatozoa, female emus laid fertilized eggs for 10.0±0.4, 12.0±0.9, and 15.0±0.6 days. When 400 million spermatozoa were used for insemination on Day 1, 2 or 3 of the oviposition cycle, the duration of the fertile period appeared to change in a day-dependent manner. After AI on Day 1, female emus laid fertilized eggs for 15.8±1.1 days, after AI on Day 2 for 12.5±2.2 days, and after AI on Day 3 for 10.0±1.5 days. The results suggest that female emus need to be inseminated the day after oviposition to maximize the duration of their fertile period.
Paraeucosmetus pallicornis (Dallas) is a new pest in Indonesia which decreases rice production and quality. This pest causes the grains to become flat, hollow, brownish, break easily when milled, and bitter. This research represents the first study on Calotropis gigantea extract as an oviposition deterrent and ovicide against P. pallicornis. The study was conducted under laboratory conditions using four extract concentrations i.e. 0.5, 1.0, 1.5 and 2.0%. The oviposition deterrent effect was determined by counting the number of eggs laid and hatched. Percent reproductive control (PRC) and ovicidal activity was calculated using Tennyson’s formula. Results indicated that all concentrations of C. gigantea leaf extract reduced the number of eggs laid and hatched. The PRC also showed a gradual reduction of oviposition of the P. pallicornis and the ovicidal activity ranged between 86.5 and 100%. The extract concentration which showed the highest potential as an oviposition deterrent and ovicide against P. pallicornis was in the range of 1.0–2.0%. The overall results indicated that C. gigantea leaf extract has the potential to be used as an oviposition deterrent and ovicide against P. pallicornis.
Insect growth regulators generally have a selective effect on the target insects and have practically no apparent side effect on non-target organisms especially vertebrates. Hence, insect growth regulators could be a suitable choice to control pests in stored products. Ten-day-old larvae of Indian meal moth Plodia interpunctella (Hübner) were expressed to the juvenile hormone analogue pyriproxyfen in order to have an effect on growth, metamorphosis, reproduction, lipid and protein contents of ovaries. The larvae were treated by 0.02, 0.04, 0.08, 0.16, and 0.3 ppm of JHA in an artificial diet where controls received acetone alone. The results indicated significant differences in duration of growth, mean longevity of hatched adults, percentage of emerged normal adults, abnormal pupae, hatched larvae and mean oviposition ratein addition to the lipid and protein of ovaries compared to the controls. An inhibition concentration of fifty (IF50) for prevention of emerging adults was recorded 0.134 ppm. Pyriproxyfen caused significant defects in the legs and wings of some adults and sever morphological changes in the ovaries of emerged adults. The results showed that pyriproxyfen may be applied as an insecticide to decrease the damage caused by Indian meal moth on stored products. Pyriproxyfen can be used with low side effects to humans.
A total number of 70 species representing 44 genera of all the subtribes within the tribe Pedinini sensu Iwan 2004 (Dendarina, Eurynotina, Platynotina, Pedinina, Melambiina, Loensina, Leichenina, Pythiopina) have been examined. The terminology of the female genital structures has been standardized. The use of the internal female genitalia in the classification of Tenebrionidae is presented. The features of the opatrinoid type of ovipositor (sensu Tschinkel and Doyen 1980) (1) dorso-lateral position of gonostylus; (2) 4 lobes of coxites; (3) paraproct partly enclosing the 1st lobe of coxite; (4) transverse orientation of baculus of 1st lobe of coxite are characterized for the examined taxa.
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