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An evaluation was made of the ossification level of the metacarpal and phalangeal bones in human foetuses of both sexes from the 4th to the 9th month of gestation. Our results indicate that ossification of phalangeal bones 1 to 5 always started at the distal end of the phalanx and endochondral ossification prevailed in the proximal phalanx of the thumb.
There is no agreement in the literature as to the time of the onset and progress of the vertebral column ossification. The aim of the present study was to determine the precise sequence of ossification of the neural arches and vertebral centra. Histological and radiographic studies were performed on 27 human foetuses aged from 9 to 21 weeks. It was found that the ossification of vertebrae commences in foetuses aged 10 and 11 weeks. Ossification centres appear first for neural arches in the cervical and upper thoracic vertebrae and by the end of 11th week they are present in all thoracic and lumbar neural arches. In the vertebral centra in foetus of 10 weeks ossification was found in the lower 7 thoracic and first lumbar vertebrae. By the end of 11th week ossification is present in the lower 4 cervical, all thoracic, all lumbar and 4 sacral vertebral centra. The study indicates that ossification of the neural arches proceeds in the craniocaudal direction, whereas in the vertebral centra it progresses from the lower thoracic vertebrae into both directions. Different shapes of ossification centres were also described. (Folia Morphol 2013; 72, 3: 230–238)
The paper presents an anatomical description of the ossified interclinoid ligament which was found in a male human skull. In the case studied the ossified ligament exists as a bony bridge between the anterior and posterior clinoid processes on the left side of the skull. The length of this connection was measured as 5.0 mm, while its thickness was 3.2 mm. We conjecture that the presence of a considerably thick bony trabecula within the sella region might have had an impact on the course of the internal carotid artery or the oculomotor nerve, causing compression of these structures.
The core of the limb bone cortex of mammals and birds is made of rapidly deposited, fibro-lamellar bone tissue (also present in non-avian theropods), which is usually surrounded by an avascular outer circumferential layer (OCL) of slowly deposited parallel-fibered bone. We present the first comparative allometric study of the relative OCL thickness (expressed as a fraction of the diaphyseal radius) in modern birds. Body size explains 79% of the OCL variation in thickness, which is inversely correlated with size, that is, shows negative allometry (slope -0.799). This may explain the apparent absence of OCL in the ratites. Since the OCL is deposited at the end of growth, we propose that its relative thickness probably correlates with the amount of slow, residual growth, which our results suggest to be on the average larger in small birds.
The aim of the study was an early diagnosis of bones with retained endochondral ossification through clinical and radiological examinations, in order to determine the factors causing the etiology of disease and to indicate necessary precautions to be taken in the prophylaxis of the resulting possible deformations. This study comprised of 51 dogs of different breeds, age and genders, brought to the clinic and complaining of gait abnormality or abnormal bone structure and which were subsequently diagnosed with retained endochondral ossification. Radiological examination indicated that while varying degrees of retained endochondral ossification were observed in the distal ulnar growth plates in all cases, retained endochondral ossification was present in the distal radial growth plate together with the ulna in one case and in all the growth plates in another case. It was established that 22 cases had left their mothers at an early age and been fed meat products and that 17 cases had received additional calcium and phosphorus in their diets. Following clinical and radiological examination, bilateral carpal valgus was diagnosed in 43 cases and bilateral carpal varus in 8 cases. Radius curvus deformation was also observed in cases where the condition had lasted for a long time. Ulnar ostectomy was performed in 18 cases diagnosed with retained endochondral ossification in the ulna and had not yet reached maturity. Seventeen cases in which the lesions were only in the beginning phase were treated by a change of diet. It was concluded that growth ceased temporarily in bones with retained endochondral ossification and that measures should be taken to avoid possible deformation, and, additionally, that feeding played a significant role in the etiology of the disease.
Ossification during embryogenesis of female and male mouse fetuses between 14.5 and 18.5 days post coilum (d.p.c.) was investigated with two methods. The Alizarinred based method allows differential staining of cartilage and bone tissue on whole animal, however standard histochemical methods allow detailed identification of cells involved in ossification process. There were minor differences in the time of occurrence of some ossification centers between male and female mouse fetuses in a period of 14.5 - 18.5 d.p.c. At 14.5 d.p.c. ossification of female but not male mandible was observed and at 18.5 d.p.c. histochemistry demonstrated ossification in the phalanges in male but not in female.
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