Wyniki wyszukiwania - AGRO

1

Variability of the position of organs in Hymenolepis diminuta tapeworms at different ages

100%

Stradowski M.

Acta Parasitologica

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1993

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tom 38

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nr 1

Hymenolepis diminuta tapeworms of the WMS "strain", obtained at 1 month and at 2.5 months after the administration to rats of cysticercoids, showed a right-hand side position of the genital pores (PGP). In the 5th month of infection strobilas of variable PGP were also found to appear. These two types of strobilas (of right-hand side and variable PGP) appeared till the end of the observation period in the 23rd month after the infection of rats. It was established that the variability of PGP depends on the duration of the infection and not on the age of the final host. Apart from typical proglottids containing 1 testis on the poral side and 2 testes on the aporal side (lp2a) there were also proglottids of the following numbers and placement of testes (NDT): 0p0a, 0pla, 0p2a, 0p3a, 0p4a, 0p5a, 1p0a, 1p1a, 1p3a, 1p4a, 1p5a, 2p0a, 2p1a, 2p2a, 2p3a, 2p4a, 3p0a and 3p1a. Correlation was established between the frequency of occurrence of PGP changes and the number of proglottids with the following NDT: 1p2a, 2p1a, 3p0a, 3p1a and all deviations from 1p2a.

2

Coenzyme Q10 as an antioxidant and its relation to HMGCoA reductase inhibitors

88%

Littarru G. P.

Polish Journal of Environmental Studies

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1997

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tom 06

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nr 2

3

Ultrastructural studies on organs of cadmium-poisoned rats treated with oxygen-ozone mixture

88%

Kuryszko J., Madej J. A., Madej P.

Archivum Veterinarium Polonicum

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1995

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tom 35

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nr 1-2

4

Changes of H2O2 level in organs of lupine seedlings exposed to lead

88%

Przymusinski R., Bartczak J., Gwozdz E. A.

Polish Journal of Natural Sciences. Supplement

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2003

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tom 01

5

Adenylate cyclase - properties, activity and methodology

75%

Jaworska-Adamu J., Cybulska R.

Folia Morphologica

|

1991

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tom 50

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nr 1-2

6

Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik

Content of sodium and potassium in tissues and organs of free-ranging European bisons

75%

Kosla T., Skibniewski M., Skibniewska E.

Journal of Elementology

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2015

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tom 20

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nr 2

The study aimed at assessing the sodium and potassium content in chosen tissues and organs of free-ranging European bisons in Białowieża Primeval Forest depending on the gender and age of animals. In order to determine the content of elements in parenchymal tissues, ribs and hair, the ICP-OES method was used. In the hooves, sodium and potassium were determined with the help of ICP-MS. The sodium content in organs and skin appendages varied from 0.30 in hair to 4.77 mg g-1 in ribs. In the analysis of the age effect, some significant differences were observed between the investigated groups in the sodium content of the hoof wall, namely, a higher mean value was noted in young individuals. The potassium content in the examined samples was within the range of 0.96 in the hoof wall to 3.63 mg g-1 in kidneys. Significant age dependent differences were noted only in the ribs. Sodium and potassium concentrations in the hoof wall were correlated in a highly significant way. Similar dependences also appeared between the content of sodium and potassium in kidneys and liver and kidneys and muscles. On the basis of the results it can be concluded that the status of sodium and potassium supply in the European bison from Białowieża Forest is adequate.

7

PIP – jaka jest? (polemika)

75%

Szewczyk J.

Ochrona Pracy

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1991

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tom 45

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nr 10

8

Wybory

75%

Jakubowska A.

Zachodni Poradnik Łowiecki. Kwartalnik Wojewódzkiego Sejmiku Łowieckiego w Poznaniu

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2010-2011

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nr 1[33]

9

Production of transgenic pigs expressing human alpha 1,2-fucosyltransferase to avoid humoral xenograft rejection

75%

Lipinski D., Jura J., Zeyland J., Juzwa W., Maly E., Kalak R., Bochenek M., Plawski A., Szalata M., Smorag Z., Slomski R.

Medycyna Weterynaryjna

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2010

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tom 66

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nr 05

316-322

The use of animals as a source of organs and tissues for xenotransplantation may overcome the growing shortage of human organ donors. However, the presence of xenoreactive antibodies in humans, directed against the swine Gal antigen present on the surface of xenograft donor cells, leads to the complement activation and an immediate xenograft rejection as a consequence of hyperacute reaction. In order to prevent a hyperacute rejection, it is possible to alter the swine genome with human genes modifying the set of the donor’s cell surface proteins. The aim of this study was to prepare a pCMVFut genetic construct and then introduce it into the swine genome in order to obtain transgenic pigs expressing human α1,2-fucosyltransferase and thereby avoid a humoral xenograft rejection. The pCMVFut gene construct containing the human gene encoding α1,2-fucosyltransferase enzyme under the human cytomegalovirus immediate early promoter was introduced by microinjection into a male pronucleus of a fertilized porcine oocyte. The screening procedure involved isolating genomic DNA from microsections of pigs’ ears, the amplification of two PCR fragments and the entire sequencing of positive samples. The mapping of the transgene was performed by fluorescence in situ hybridisation (FISH) and transgene expression, while its impact on the reduction of the Gal epitope level on the surface of pig cells was assessed by flow cytometry of primary cultured skin fibroblasts. The influence of the human complement was measured by testing the sensitivity of nontransgenic and transgenic cells to complement-mediated cytotoxicity upon exposure to human serum. As a result of this experiment, the founder male pig was obtained with the transgene mapping to chromosome 14q28. An RT-PCR analysis revealed the expression of the HT gene in different tissues of transgenic pigs. A flow cytometry analysis revealed a reduction in the level of epitop Gal on the cell surface of skin fibroblasts isolated from transgenic pigs. The complement-mediated cytotoxicity assay showed increased viability of transgenic cells in comparison with nontransgenic ones, which confirmed the protective influence of HT expression. In this study we demonstrated that the constitutive transgenic expression of human H-transferase (α1,2-fucosyltransferase) can decrease the amount of Galα1,3Gal (Gal epitope) on the surface of pig cells, which is consistent with the results of other researchers. The expression of α1,2-fucosyltransferase modified the cell surface carbohydrate phenotype of transgenic pig cells, resulting in the expression of the universally tolerated 0 blood group antigen (H antigen) and a subsequent reduction in the expression of Gal epitope, as evaluated by flow cytometry analysis. Apart from the principal data, the flow cytometry analysis revealed no significant differences between the Gal epitope level achieved by CMVFUT heterozygous boar founder TG 1154 and transgene homozygous pig 433 from the F2 generation. The flow cytometry results were confirmed by the cytotoxicity assay. We found no statistical difference in the survival rate between transgenic homozygous and heterozygous cells under the influence of 50% human serum with an active complement system. Both homozygous and heterozygous cells had the same level of lysis protection.

10

Accumulation dynamics of PCBs in selected organs and tissues of carp [Cyprinus carpio L.]

75%

Ciereszko W.

Acta Ichthyologica et Piscatoria

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2001

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tom 31

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nr 2

The study was aimed at the PCB (Clophen A50) accumulation dynamics in the gill filaments, muscle tissue, perintestinal adipose tissue, alimentary tract and the liver of cultured carp, Cyprinus carpio L. when taken, only, either from contaminated water or contaminated diet. The highest accumulation dynamics was observed in first 5-10 days of intoxication followed by a visible decrease resulted partly from increase in a growth rate of the tested organs and tissues as well as from the smaller daily intake per weight unit of the tested fish.

11

Effect of low frequency electromagnetic fields on [3H]glucose uptake in rat tissues

75%

Sieron A., Brus H., Konecki J., Cieslar G., Szkilnik R., Nowak P., Noras L., Kwiecinski A., Kostrzewa R. M., Brus R.

Polish Journal of Environmental Studies

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2007

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tom 16

|

nr 2

The aims of this study were to evaluate the influence of an extremely low-frequency electromagnetic field (ELF-EMF) on [³H]glucose uptake in the peripheral tissues and organs of rats. Rats were exposed to EL F-EM F (frequency-10 Hz, induction -1.8-3.8 mT) one hour daily for 14 consecutive days. Control animals were sham exposed. On the 15th day (24 hours after last exposure) rats were injected with D-[³H]-6-glucose 500μCi/kg IP. Fifteen minutes later animals were sacrificed by decapitation and peripheral tissues were excised and examined for radioactivity (desintegrations per minute, DPM/100 mg wet tissue weight), which expressed [³H]glucose uptake. In most of the examined tissues and organs, such as liver, kidney, heart muscle, cartilage, connective tissue, tendon and skin, [³H]glucose uptake in EL F-EM F-exposed animals was significantly higher as compared to that in the sham control. Exposure to EL F-EM F did not influence [³H]glucose uptake in the thoracic aorta and the skeletal muscle. It is concluded that ELF-EMF impacts tissue glucose uptake by facilitating glucose transport via cell membranes, dependent and probably also independent of its role in increasing insulin action in insulin-dependent tissues.

12

Heavy metals in the organs of anseriform birds

75%

Danczak A., Ligocki M., Kalisinska E.

Polish Journal of Environmental Studies

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1997

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tom 06

|

nr 5

The anseriform birds studied in the present work were taken from Słońsk Reserve, the Baljic Seacoast and from a breeding farm in Kołuda Wielka. The contents of metals (lead Pb, cadmium Cd, zinc Zn, copper Cu) in the livers and kidneys of male and female ducks (Anas platyrhynchos, mallard; Clangula hyemalis, long-tailed duck) and geese (Anser anser, greylag goose; A.anser f.domestica, domestic goose) were compared. The highest Cd level was found in the kidneys of mallards from the Słońsk Reserve, the lowest in the kidneys of domestic geese. Moreover, the highest levels of Pb, Zn and Cu were recorded in mallards. Analyzing the contents of metals in the livers of tested birds, the highest levels of Pb, Cd and Zn were also found in mallards and geese from Słońsk. The highest Cu level was found in the livers of domestic geese. The levels of all studied metals, except copper, was higher in the livers of females.

13

The accumulation of chlorinated hydrocarbons in selected organs of fresh-water fish after exposure to gamametox powder

75%

Kiziewicz B.

Polish Journal of Environmental Studies

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1993

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tom 02

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nr 2

Gas chromatography was used to determine lindan and methoxychloride residues in the muscle, brain and liver of selected fish species caught in Popielewo pond in Białystok province. The fish (Carassius carassius L, Cyprinus carpio L, Leucaspius delineatus Heckel) were exposed to an effective concentration of 0,5 ppm Gamametox Powder in water for 30 days. The experiment showed that the highest accumulation of lindan and methoxychlor was in the brain. In the brain of Cyprinus carpio the level was up to 2.41 ppm lindan. The level methoxychlor in livers was similar to the level of this pesticide in the brain, while the level of lindan in livers was considerably the lowest The lowest residues of lindan and methoxychlor accumulation were observed in muscle, where the levels were up to 0.0149 and 0.0351 ppm (Leucaspius delineatus and Cyprinus carpio). Organochlorine pesticide was detected in samples offish from Popielewo pond (control group), but this level was very low. Therefore, it is suggested that the natural environment (Popielewo pond) also contains low concentrations of organochlorine.

14

The systemic changes in content of free amino acids in the organs of winter triticale induced by grain aphid [Sitobion avenae F.] feeding

75%

Ciepiela A. P., Sempruch C., Michalak A.

Polish Journal of Natural Sciences. Supplement

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2003

|

tom 01

15

Influence of long-term nitrate exposure on calves

75%

Baranova M., Mal'a M., Burdova O., Zezula I.

Bulletin of the Veterinary Institute in Puławy

|

1999

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tom 43

|

nr 1

A long-term nitrate load was given to calves by oral administration of aqueous solution of KNO₃ to 6 five-week-old experimental calves. The calves were divided into two groups, three animals in each. During a period of six weeks the calves in the experimental group 1, received 2 g of KNO₃ every day, while those of the 2nd group were administered 5 g of KNO₃ per day. After 6 week application, when the weight of the calves reached 75 kg on average, the doses administered were increased to 5 g in the first and 10 g in the second group. The application lasted additional 20 days. The determination of the percentage of methaemoglobin in the blood of the experimental and control calves in the course of the experiment was used to investigate the response of the calves to orally administered nitrate. The mean values of MtHb determined on the last day of administration were lower in both experimental groups of calves than those detected on the first day of the administration of the nitrate (Figs 1-3). The long-term intake of nitrate in a dose of 2-10 g KNO₃, per head daily was not reflected in a deteriorated state of health or in the decreased weight gain of experimental animals in comparison with the control. In order to determine the distribution of the substances administered, the content of residual nitrate and nitrite in the muscles and organs of experimental and control animals was also determined (Figs 4-5).

16

Activities of glutathione peroxidase and catalase in organs from vanadium-treated rats

63%

Krosniak M., Krzysciak W., Kareta A., Grybos R.

Acta Biologica Cracoviensia. Series Zoologia

|

2009

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tom 51

39-48

17

Effect of bentonite on supplemental zinc uptake and distribution in rats

63%

Grosicki A., Rachubik J.

Bulletin of the Veterinary Institute in Puławy

|

2010

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tom 54

|

nr 4

Rats were offered ad libitum zinc in twice the level of the standard diet (23 mg/kg diet), and bentonite (2% additive) for 28 d, together with traces of zinc chloride (labelled with zinc-65) given intragastrically. Results provided evidence that bentonite increased the body retention of zinc in comparison to the diet without this agent. Furthermore, the addition of bentonite did not influence feed intake, organ to body ratios, and haematological values, although a visible decrease in body weight gains following bentonite feeding was noted. These findings may be useful when bentonite fertilised diet is provided to animals pastured in areas with higher zinc levels.

18

Results of long-standing mycological analyses of biological materials originating from selected organ ontocenoses - yeast and yeast-like fungi

63%

Dynowska M., Goralska K., Troska P., Baranska G., Biedunkiewicz A., Ejdys E., Sucharzewska E.

Wiadomości Parazytologiczne

|

2011

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tom 57

|

nr 2

This paper reviews the results of an extensive monitoring study, spanning 20-years of observation from three medical centres in Olsztyn on the dynamics and species diversity of fungi most frequently colonizing the respiratory and digestive systems of humans. The experimental materials were swabs and specimens from the gastrointestinal tract, swabs from the oral cavity and pharynx, as well as sputum and bronchial fluid from the respiratory system. The biological material was subjected to routine mycological diagnostics, taxonomic determination and identification. In total, 41 species of yeast and yeast-like fungi were isolated, including 34 from the respiratory and 25 from the digestive system. In the last decade, a significant increase has been noticed in the counts of fungi, especially the gastrointestinal tract, reported from people. As many as 18 species were isolated from both systems – they were predominated by fungi of the genus Candida and their perfect forms. Worthy of notice are also frequent isolations of yeast (Saccharomyces spp.) and detection of an endemic species, Paracoccidioides brasiliensis, in the respiratory system, and of the sexual stages of Rhodosporidium diobovatum and Rhodosporidium kratochvilovae from the gastrointestinal tract.

19

The effect of natrium fluoride on selected internal rat organs - an ultrastructural study

63%

Dabrowska E., Balunowska M., Szynaka B., Letko R.

Acta Toxicologica

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2005

|

tom 13

|

nr 1

20

Absence of CSF-1 in macrophage - deficient op-op mice has differential effects on macrophage colony stimulating activity [M-CSA] released by various organs and cells

63%

Wiktor-Jedrzejczak W., Sell K. W., Szwech P., Ahmed-Ansari A.

Folia Histochemica et Cytobiologica

|

1995

|

tom 33

|

nr 4

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