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Results of experiments with isolates A1 and A2 from populations occurring in Poland in the years 1993-1998 were compared. Mating types A1 and A2 did not significantly differ with respect of virulence spectrum, pathogenicity level and virulence diversity (Shannon index). After pairing isolates A1 and A2 formed oospores; their morphological characteristics were described. On selected fields a test was performed whether the oospores could play a role as a source of primary infection. Local populations occurring on three fields were characterized. Results obtained on investigated 3 separate fields, located in 2 voivodeships of southern Poland were compared. It was stated on the basis of the proportion of both mating type isolates, race complexity and diversity, that oospores could play a role as a source of primary infection, at least in one of localities in Boguchwała, podkarpackie voivodeship.
The morphological variation of Chara rudis A. Braun oospores was examined in a local population in a deep (43 m), hardwater (34 mg Ca dm–3) and mesotrophic (Ptot 0.095 mg P dm–3, SD 8 m) lake (NW Poland). Two variants of population density and three variants of depth were taken into account. Oospores are 712.8 ± 37.6 (600–817) μm long and 452.6 ± 34.2 (350–516) μm wide. They have 8–13 ridges. The outer membrane is slightly granulated. The isopolarity index (length/width × 100) is 158.4 ± 9.9 (140–190). The variation coefficient varies from 5% for oospore length to around 10% for fossa width. It was shown that the differences between oospores coming from individual depth and density variants are statistically significant. The biggest (733.21 ± 33.3 μm) oospores occurred on individuals growing at a depth of 3 m, while the smallest ones (673.3 ± 43.4 μm) at 5 m. The oospores from highly dense aggregations (> 50 individuals × 0.1 m–2) were much longer and wider than oospores from scattered (<10 individuals × 0.1 m–2) individuals. The observed intrapopulation variation of oospores can be regarded as a response to environmental changes along the depth gradient.
Morphological features of oospores of Chara baueri A. Braun, one of the rarest charophyte species worldwide, were studied based on 100 oospores collected from a small and temporarily dried mid-field pond near Cedynia, Western Poland. This is the first Polish and fifth presently known locality of this species. For comparison 67 oospores from a German population (similar pond localized near Batzlow, Germany) were also measured. So far, data on morphology of C. baueri oospores as well as the species ecology are limited. The only more detailed study of oospores for this species was earlier performed on 15 oospores from Kazakhstan. Largest polar axis (LPA, length), largest equatorial diameter (LED, width), isopolarity index (ISI = LPA/LED × 100), number of ridges, width of fossa, distance from apical pole to LED (AND) and anisipolarity index (ANI = AND/LPA × 100) were measured. The comparative analysis revealed that the oospores from Poland are generally bigger and more prolate than the German ones. The differences for most of studied parameters were statistically significant. The finding is discussed in the context of habitat differentiation of both studied sites. Moreover, the results obtained of oospore measurements for both populations differs from most of the data known so far from the literature.
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