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Isolation and expression of CsFAD7 and CsFAD8, two genes encoding omega-3 fatty acid desaturase from Camellia sinensis

100%

Ma Q.-P., You E., Wang J., Wang Y., Ding Z.-T.

Acta Physiologiae Plantarum

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2014

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tom 36

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nr 09

The plastidial ω-3 fatty acid desaturase catalyses the production of trienoic fatty acids (TAs) in plant chloroplasts and plays an important role in plant responses to environmental stress. In this study, the full-length cDNAs encoding two plastidial ω-3 desaturases, designated CsFAD7 and CsFAD8 (GenBank Accession No. JX943516 and KC847167, respectively), were isolated from the tea plant (Camellia sinensis L.) using RT-PCR and RACE. Codon usage analysis revealed that U- and A-ended codons were preferentially used in these two genes. Sequence analysis showed that the deduced amino acid sequences of CsFAD7 and CsFAD8 had high homology to plastidial ω-3 desaturases from other plant species. Expression analysis by real-time PCR revealed that both genes are tissue-specific and expressed the highest levels in shoots. Meanwhile, CsFAD7 and CsFAD8 responded to various abiotic stresses and hormones, but in very different manners. Taken together, these results suggest that CsFAD7 and CsFAD8 are both responsive to abiotic stress signals; however, they may play very different roles during stress tolerance in tea plants.

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Genetic resources of flax (Linum usitatissimum L.) as very rich source of alpha-linolenic acid

100%

Silska G.

Herba Polonica

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2017

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tom 63

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nr 4

Introduction: Polish oilseed and flaxseed collection is a source of genotypes containing very high amounts of α-linolenic acid. Objective: The objective of the study is to test the seeds for the fat content and fatty acids composition in the oil pressed from the 9 tested accessions of flax (Linum usitatissimum L.). Our goal is to promote the Polish flax collection, which seeds are unique as one of the richest sources of α-linolenic acid. Methods: Assays to determine the content of fat and fatty acids composition in linseed oil were performed at the IHAR-PIB Biochemical Laboratory in Poznań. The fat content was determined by infrared analysis (calibration performed on the basis of seed sample at IHAR-PIB in Poznań) by means of a NIRS 6500 spectrophotometer with a reflection detector within the range of 400–2500 nm. The composition of fatty acids was determined by means of a method proposed by Byczyńska and Krzymański (1969), based on gas chromatography of methyl esters of fatty acids contained in linseed oil. The following varieties of flax were investigated: Tabare (INF00111), Szegedi 30 (INF00427), Olin (INF 00444), Redwood 65 (INF00523), Dufferin (INF00540), AC Mc Duff (INF00648), Alfonso Inta (INF00683), Olinette (INF00687), Royale (INF00689). Results: The content of α-linolenic acid (ALA, C18:3) in evaluated genotypes of flax ranged from 48.9 (Royale) to 59.9% (Alfonso Inta). Content of linoleic acid (LA, C18:2) in evaluated genotypes of flax ranged from 12.4 (Tabare) to 17.1% (AC Mc Duff). The content of oleic acid (OA, C18:1) of 9 accession of flax ranged from 17.1 (Alfonso Inta) to 26.7% (Royale). The content of stearic acid in evaluated genotypes of flax ranged from 2.3 (Alfonso Inta) to 5.0% (Tabare, Szegedi 30) and the content of palmitic acid ranged from 4.7 (Dufferin) to 6.0% (Olin). The content of fat ranged from 42.7 (Olin) to 52.0% (AC Mc Duff). The fatty acid ratio n-6/n-3 ranged from 0.23/1 (Tabare) to 0.32/1 (AC Mc Duff).

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The effect of omega-3 fatty acids on expression of connexin-40 in Wistar rat aorta after lipopolysaccharide administration

100%

Frimmel K., Vlkovicova J., Sotnikova R., Navarova J., Bernatova I., Okruhlicova L.

Journal of Physiology and Pharmacology

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2014

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tom 65

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nr 1

4

Effect of nanoencapsulation on the reactivity of omega fatty acids under thermal processing conditions

100%

Mogol B. A., Gokmen V., Lumaga R. B., Fogliano V., Shimoni E.

Polish Journal of Food and Nutrition Sciences

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2011

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tom 61

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nr Suppl.1

5

Chia seeds (Salvia hispanica): health promoting properties and therapeutic applications - a review

84%

Marcinek K., Krejpcio Z.

Roczniki Państwowego Zakładu Higieny

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2017

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tom 68

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nr 2

Chia has been known for over 5,500 years. Chia seeds were one of the most important components of the diet of Mayas and Aztecs. The chemical composition and technological properties of chia give the plant a high nutritional potential. Chia is a good source of polyunsaturated fatty acids: omega-3 and omega-6, soluble dietary fiber. It also contains appreciable amount of proteins and phytochemicals. Nutritional value of chia is the reason why it is used in prophylaxis of several non-infectious diseases such as obesity, hypertension, cardiovascular diseases (CVDs), cancer and diabetes. Nutritional and therapeutic aspects of chia are currently being researched by many scientific centres. The aim of this article is to present the nutritional and therapeutic values of chia.

6

Health-oriented cold meats

84%

Woicka-Bekas E.

Polish Food

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2010

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nr 1

7

Influence of a diet containing beta-carotene and omega-3 fatty acids on the biochemical and nonspecific humoral immunity indicators and on the results of experimental calf rearing

84%

Zwierzchowski G., Micinski J., Pogorzelska J., Siwicki A., Wojcik R., Kobzhassarov T. Z., Bermagambetova N., Shaikamal G. I., Fijalkowska M.

Journal of Elementology

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2016

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tom 21

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nr 1

The aim of this study has been to determine the effect of a diet containing β-carotene and omega-3 fatty acids on the biochemical and nonspecific humoral immunity indicators and on the results of rearing calves to day 60 of life. The study was conducted in 2011-2012, on a herd of Polish Holstein-Friesian cows. 54 calves from the herd were divided into 3 groups, 18 animals in each: CTR, TRT1 and TRT2. During the colostral period lasting for 5 days after birth, calves were drinking the mother’s colostrum. From 6 to 60 days, calves were watered milk replacer formulation. The control group (CTR) consisted of calves after the colostral period given milk replacer formulation without any supplementation. In the two other groups, calves received milk replacers with the supplementation of b-carotene (TRT1 group) in the amount of 25 mg/calf/day or a suspension of liver omega-3 oil (TRT2 group) in the amount of 5g/calf/day. All calves received solid feed ad libitum. The research showed that the parameters of the metabolic profile of calves (ALT, ASP, CHOL, GLU, UREA, ALP, TG, CRE) were within the normal range, indicating good health of the calves. Moreover, the supplementation had a positive impact on the chosen immunological parameters, including a higher IgG concentration, especially at the end of the milk drinking period. The result of B-carotene in a diet was the highest daily gain in the period from 30 to 60 days of life among the analysed groups of calves. No such effect was achieved in the TRT2 group of calves, as their body weight was the lowest. However, noteworthy is the beneficial impact of beta-carotene and omega-3 oil on the non-specific humoral immunity parameters, which was manifested by fewer cases of clinical diarrhoea and upper respiratory tract diseases.

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The physical traits and fatty acids profile of ostrich meat enriched in n3 fatty acids as influenced by duration of refrigerated storage and type of packaging

84%

Polawska E., Poltorak A., Wyrwisz J., Wierzbicka A., Gutkowska K., Pomianowski J., Zdanowska-Sasiadek Z., Wojcik A., Kawka M., Raes K., De Smet S.

Animal Science Papers and Reports

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2014

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tom 32

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nr 4

The aim of the study was to evaluate physical traits and fatty acids profile of ostrich meat enriched in n3 fatty acids as affected by refrigerated storage (for 14 days) and type of packaging (VAC vs. skin-packaging – SP). During refrigerated storage time drip loss after 7 days was significantly (P<0.001) higher in VAC as compared to SP samples. No significant differences in the SFA content in meat during storage in both types of packaging types recorded. Although there were no significant differences (P=0.067), a tendency for higher MUFA values was observed during storage in VAC packed meat. A significant decrease (P<0.05) in the content of PUFA after 7 and 14 days of storage was also observed in VAC packed meat as compared to fresh meat, whereas, when skin packaging was used, no differences in the PUFA concentration were found. Considering this, the SP can be recommended packaging for ostrich meat industry.

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The role of dietary fats for preventing cardiovascular disease. A review

84%

Szostak-Wegierek D., Klosiewicz-Latoszek L., Szostak W. B., Cybulska B.

Roczniki Państwowego Zakładu Higieny

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2013

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tom 64

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nr 4

At the present, there is a pandemic of chronic non-communicable disease (NCD) affecting most countries of the world. The World Health Organisation (WHO) has identified the main contributing determinants to be cardiovascular disease (CVD), diabetes, malignant cancer and chronic disease of the respiratory system. Unhealthy nutrition, as well as other adverse lifestyle health behaviour are recognised to be part of the prime factors responsible. According to WHO guidelines, a healthy lifestyle should include substituting saturated fatty acids (SFAs) with polyunsaturated fatty acids (PUFAs) together with eliminating trans-fatty acids from the diet and limiting the intake of refined carbohydrates in conjunction with increasing the consumption of fruit, vegetables, nuts and wholegrain cereal products. Recent studies on the relations between CVD prevention and dietary fats have been however unclear. The present study thus aims to provide a review of current evidence and opinion on the type of dietary fat most appropriate for preventing arteriosclerosis. The adoption of dated recommendations on the need to increase dietary PUFA in both Northern Europe and America has led to n-6 PUFAs being predominant in diets as compared to n-3 PUFAs. This disproportion may have caused mortality to rise, due to CVD, as a result of arteriosclerosis in these countries. In contrast, a traditional Mediterranean diet yields a PUFA n-6/n-3 ratio of 2:1, which is much lower than for the aforementioned northern countries. Some authors however consider that assessing this ratio is irrelevant and that decreasing n-6 PUFA may be harmful. Such differences of opinion leads to confusion in adopting an effective approach for arteriosclerosis management regarding dietary n-6/n-3 ratios. Moreover, recent studies have added much controversy to the notion that the characteristics of SFAs are responsible for arteriosclerosis. These found that replacing dietary SFAs with carbohydrates did not reduce the risk of ishaemic heart disease (IHD). Furthermore, changing to monounsaturated fatty acids (MUFAs) gave equivocal findings, but only changing to PUFAs reduced the risk of IHD. This last statement however requires qualification in that dietary n-6 PUFAs increases the risk of IHD. It is only the n-3 PUFAs that are beneficial. Up till now these controversies remain unsolved. It is however noteworthy that adopting a Mediterranean diet reduces IHD mortality. This is explained by a low consumption of SFAs but high intake of unsaturated fatty acids including n-3 PUFAs, and is linked to choosing the right vegetable fats. Oils that contain a-linoleic acid (ALA) are to be preferred in the diets of northern countries.

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Eicosapentaenoic acid as mitochondria protective agent in endothelial cells exposed to stress

84%

Goralska J., Sliwa A., Zapala B., Gruca A., Czech U., Awsiuk M., Dembinska-Kiec A.

Polish Journal of Food and Nutrition Sciences

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2011

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tom 61

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nr Suppl.1

11

Characterization of fatty acid composition in the European beaver [Castor fiber L.]

84%

Zalewski K., Martysiak-Zurowska D., Chylinska-Ptak M., Nitkiewicz B.

Polish Journal of Environmental Studies

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2009

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tom 18

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nr 3

493-499

Lipids obtained from the muscular and adipose tissues of the European beaver were isolated by the modified Folch method. Fatty acids were converted to methyl esters and separated by high-resolution gas chromatography (HR-GC). The content and composition of beaver fat depended on the sex of a given animal. The adipose subcutaneous tissue of the female contained the most fat (approximately 70.5%). The fat content of muscular tissue was very low in both male and female beavers. Adipose tissue lipids of the beaver contained fatty acids ranging in chain length from 12 to 22 carbon atoms. Polyunsaturated fatty acids have the highest proportion in of total FA content of adipose tissue in the beaver, which distinguishes these lipids from the lipids of adipose tissues in other mammals. The results of the present study also confirmed a unique FA composition in the tail fat of the beaver, including a very high content of alpha-linolenic acid (ALA, 18:3 n-3) (on average 20.0%) and the sum of n-3 fatty acids (on average 20.45%). In addition, a very low content of the sum of saturated fatty acids (on average 14.93%) was observed, and an extremely low content, as for animal fat, of palmitic acid 16:0 (on average 10.53%).

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Modulation of connexin-43 by omega-3 fatty acids in the aorta of old spontaneously hypertensive rats

84%

Dlugosova K., Okruhlicova L., Mitasikova M., Sotnikova R., Bernatova I., Weismann P., Slezak J., Tribulova N.

Journal of Physiology and Pharmacology

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2009

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tom 60

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nr 3

63-69

Hypertension alters expression of connexin-43 (Cx43) in cardiovascular system. The aim of the study was to investigate the effect of omega-3 polyunsaturated fatty acids (30 mg/day for 2 months) on expression of Cx43 in the aorta of 1-year-old male spontaneously hypertensive rats (SHR). Spatial distribution and expression of Cx43 in aortic wall of SHR and age-matched Lewis rats were determined by immunofluorescent method and Western blot. NO synthase (NOS) activity and endothelium-dependent relaxation of the aorta were measured as well. Immunofluorescent pattern of Cx43 was identified in endothelial and smooth muscle cells of the aorta of all experimental groups studied. However, local decrease in the number and intensity of fluorescent spots and reduced phosphorylation of Cx43 were observed in SHR in contrast to normotensive LEW. Omega-3 fatty acid diet increased Cx43 immunolabeling in endothelium and media of SHR comparing to untreated ones. Parallel, 3-fatty acids significantly elevated phosphorylation of Cx43 in the aorta of SHR (p<0.001). Despite the omega-3 fatty acids reduced blood pressure and stimulated aortic NOS activity in SHR, endothelium-dependent relaxation of the aorta did not significantly change. Results indicate that the aorta of old SHR might partially benefit from 3-PUFA supplementation due to increased Cx43 phosphorylation, NOS activity and decreased blood pressure.

13

Comparison of sensory quality of omega-3 fatty acids enriched and conventional eggs and frankfurters

84%

Modzelewska-Kapitula M., Zywica R.

Towaroznawcze Problemy Jakości. Polskie Towarzystwo Towaroznawcze

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2017

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nr 2

14

Capillary zone electrophoresis [CZE] of flaxseed phenolic constituents - a short report

84%

Rybarczyk A., Pegg R. B., Amarowicz R.

Polish Journal of Food and Nutrition Sciences

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2008

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tom 58

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nr 2

Phenolic compounds were extracted from flaxseed using 80% (v/v) methanol. The resultant crude extract was hydrolysed under basic conditions. Secoisolariciresinol diglucoside (SDG) was separated from the prepared extract using semi-preparative HPLC. Secoisolariciresinol (SECO) was liberated from SDG by acid hydrolysis. The chemical structures of SDG and SECO were confirmed by ESI-MS. Capillary zone electrophoresis (CZE) was employed for the separation of phenolics from the crude extract, the extract resulting after base hydrolysis, and the two lignans. CZE was found to offer a quicker analysis of SDG with higher separation efficiency than with a conventional HPLC methodology. The retention time for SDG was noted at 5.33 min. The migration times of SDG and SECO were close together; a base line separation of both lignans was not achieved. The electropherogram of the crude flaxseed extract showed only one broad peak with a retention time of 6.02 min.

15

Systemic immune-inflammation index (SII) and neutrophil to lymphocyte ratio (NLR) are useful markers for assessing effects of anti-inflammatory diet in patients before coronary artery bypass grafting

67%

Szymanska P., Rozalski M., Wilczynski M., Golanski J.

Roczniki Państwowego Zakładu Higieny

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2021

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tom 72

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nr 3

16

Omega-3 fatty acids and atorvastatin suppress ventricular fibrillation inducibility in hypertriglyceridemic rat hearts: implication of intercellular coupling protein, connexin-43

67%

Bacova B., Radosinska J., Knezl V., Kolenova L., Weismann P., Navarova J., Barancik M., Mitasikova M., Tribulova N.

Journal of Physiology and Pharmacology

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2010

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tom 61

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nr 6

17

Quality of lipids in marinated herring

67%

Domiszewski Z., Bienkiewicz G., Plust D., Kulasa M.

Electronic Journal of Polish Agricultural Universities. Series Food Science and Technology

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2011

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tom 14

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nr 1

18

Halomonas sp. nov., an EPA-producing mesophilic marine isolate from the Indian Ocean

67%

Salunkhe D., Tiwari N., Walujkar S., Bhadekar R.

Polish Journal of Microbiology

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2011

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tom 60

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nr 1

Marine samples from the Indian Ocean were used to isolate and characterize the organisms with respect to their fatty acid profiles. Six mesophilic isolates (MBRI 6, MBRI 8, MBRI 9, MBRI 10, MBRI 12 and MBRI 13) were obtained from three different water samples. They were i) Gram-negative, ii) catalase positive, iii) produced acid from glucose and maltose, iv) tolerated 5 to 15% NaCl v) except MBRI 9, showed pH tolerance in the range of 5.0 to 9.0 with optimum pH 7.0 to 8.0 v) grew well at 30°C and were able to grow in the range of 15 to 45°C. EPA, an essential omega-3 fatty acid, was produced by these isolates in the range of 12 to 60% at 30°C. MBRI 12 was found to be a potential source as it produced 60% EPA. This isolate was further identified by partial 16S rDNA sequencing and phylogenetic analysis revealed that the strain belonged to Gammaproteobacteria and was closely related to Halomonas bolviensis (96% sequence similarity, 570 bp). Thus a new genus of Halomonas may be included in earlier reported EPA- producing prokaryotic genera affiliated to the Gammaproteobacteria.

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Ocena jakosci zywieniowej suplementow dlugolancuchowych wielonienasyconych kwasow tluszczowych omega-3 obecnych na polskim rynku farmaceutycznym

67%

Kolanowski W., Mowinska W.

Bromatologia i Chemia Toksykologiczna

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2006

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tom 39

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nr 2

155-164

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The necessity of adequate nutrition with diets containing omega-3 and omega-6 fatty acids for proper brain development, function and delayed aging: Review

67%

Kochman K., Czauderna M.

Journal of Animal and Feed Sciences

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2010

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tom 19

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nr 4

Ograniczanie wyników

Isolation and expression of CsFAD7 and CsFAD8, two genes encoding omega-3 fatty acid desaturase from Camellia sinensis

Genetic resources of flax (Linum usitatissimum L.) as very rich source of alpha-linolenic acid

The effect of omega-3 fatty acids on expression of connexin-40 in Wistar rat aorta after lipopolysaccharide administration

Effect of nanoencapsulation on the reactivity of omega fatty acids under thermal processing conditions

Chia seeds (Salvia hispanica): health promoting properties and therapeutic applications - a review

Health-oriented cold meats

Influence of a diet containing beta-carotene and omega-3 fatty acids on the biochemical and nonspecific humoral immunity indicators and on the results of experimental calf rearing