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The potential of mycorrhization of lettuce in hydroponic conditions was tested in a two-year experiment carried out in the autumn of 2011 and 2012 in an unheated foil tunnel. The experimental factors were as follows: inoculation of plants with arbuscular mycorrhizal fungi (AMF+ or AMF-), concentration of phosphorus in a nutrient solution (10 or 40 mg dm-3) and the type of substrate (rockwool or coconut coir). The inoculation of plants with arbuscular mycorrhizal fungi of the genus Glomus was performed twice, i.e. while sowing seeds and when planting lettuce plants into mats. The impact of the experimental factors on the degree of colonization of lettuce roots by AMF as well as the yield and nutritional status of plants, expressed as the content of macro- and micronutrients, was studied. The mycorrhizal frequency in roots inoculated with AMF reached 51.36%. The nutrient solution concentration of phosphorus and the type of substrate did not have any significant effect on the mycorrhizal frequency. There were no differences in the yield and content of macronutrients in lettuce leaves between plants inoculated and not inoculated with AMF (AMF+, AMF-). Likewise, there were no differences in the yield caused by different phosphorus levels in the nutrient solution or the types of substrate. Plants growing on rockwool accumulated significantly less P, Ca and Mg in comparison to plants growing on coconut coir. Regarding Ca, this effect was especially pronounced in plants receiving the nutrient solution with a higher concentration of P, i.e. 40 mg dm-3. Mycorrhiza had a significant effect on the content of Cu, Mn and Zn in lettuce leaves. Inoculated plants were characterized by a lower content of these micronutries compared to plants grown without AMF. With respect to Mn, this effect was particularly evident in plants grown on rockwool.
Vegetative experiments were carried out in the years 2002–2004 in two specialistic horticultural farms growing the most popular in Poland and in the Netherlands cultivars of anthurium (Anthurium cultorum Birdsey): ‘Baron’, ‘Choco’, ‘Midori’, ‘Pistache’, ‘President’ and ‘Tropical’. Plants were grown in expanded clay with the use of drop fertigation with standard nutrient for anthurium in inert substrates (in mg‧dm⁻³): N-NH₄ < 14.0, N-NO₃ 105.0, P 31.0, K 176.0, Ca 60.0, Mg 24.0, S-SO₄ 48.0, Fe 0.840, Mn 0.160, Zn 0.200, B 0.220, Cu 0.032, Mo 0.048, pH 5.5–5.7, EC 1.5 – 1,8 mS‧cm⁻¹. Subject of studies was the differentiation of microelement content in drainage waters driped from the substrate in relation to the supplied nutrient. Manganese was the nutrient wich was most intensely decreased (by –65.5%), followed by iron (by –51.9%) and zinc (by –45.2%). On the other hand, an increase was found in copper (by +11.1%) and in boron (by +16.6%). The recognition of changes in the contents of nutritive components in the drainage waters is a basis for the elaboration and implementation into the horticultural practice of closed fertigation systems with nutrient recirculation.
Vegetation studies were conducted from 2002 to 2004, in two leading farms, located near Poznan where have obtained optimum anthurium (Anthurium cultorum Birdsey) yielding. Plants were grown in expanded clay with the application of fertigation with a standard nutrient solution (in mg·dm⁻³): N-NH₄<14.0, N-NO₃ 105.0, P 31.0, K 176.0, Ca 60.0, Mg 24.0, S-SO₄ 48.0, Fe 0.840, Mn 0.160, Zn 0.200, B 0.220, Cu 0.032, Mo 0.048, pH 5.5 – 5.7, EC 1.5 – 1.8 mS·cm⁻¹. Significant changes of macroelement contents were found in drainage water in comparison to nutrient solution. The most decreased contents (in %) of macroelements were found for phosphorus (51.2), potassium (26.7) and nitrogen (15.4). The EC value showed a downward trend of about 9.5%. Nutrients which concentration increased in drainage water were (in %): calcium (27.9), sulphur (14.3) and magnesium (5.5). The knowledge of variations of nutrient concentrations in drainage waters is the basis for the practical application of closed fertilization systems with the recirculation of nutrient solution.
Greenhouse experiments with tomato were conducted in the years 2004-2006. The aim was to study the effect of nutrient solution pH on the availability of phosphorus in soilless culture of tomato. Tomato plants were grown on mats made of shredded rye straw, and on slabs of peat and rockwool. The plants were fertilized with a standard nutrient solution of different pH: 4.5, 5.0, 5.5, 6.0 and 6.5. Such different pH levels of the nutrient solution used in soilless culture modified the pH of the root growth zone. This effect was more evident in rockwool than in the organic media (especially straw). The phosphorus content in the root zone was closely associated with the pH value of the supplied nutrient solution and the kind of growing medium. Considerably lower concentrations of phosphorus were recorded in the organic media (straw, peat) than in rockwool. As the pH level of the nutrient solution increased, so did the pH of the root zone, whereas the amount of available P-PO4 decreased. The course of these changes was similar in all the substrates. However, the dynamics of the changes was weaker in the organic media. With an increase in the pH of the nutrient solution, the average phosphorus content in tomato leaves decreased. The P-PO4 concentration was higher in the leaves of tomato plants growing in rockwool compared to those in the organic media. A significantly higher marketable yield was obtained from tomato plants cultivated in rockwool than in the organic media. For all the growing media, the highest yield of tomatoes was obtained after feeding the plants with the nutrient solution of pH 5.5.
Effects of growing media and concentration of nutrient solution on growth, flowering, evapotranspiration and macroelement content of media and leaves of Tymophylla tenuiloba were evaluated under ebb-and-flow conditions. Two media: peat and peat + perlite (3: l, v/v), and four concentrations of nutrient solution: 1.0, 1.5, 2.0, 2.5 mS cml were applied. High quality plants were produced in both media and all concentration of nutrient solution. The lowest evapotranspiration was measured at the highest concentration of nutrient solution. N concentration of leaves was high in all treatments. Concentrations of K, Ca, and Mg decreased with increasing concentration of nutrient solution. Opposite was found for P. At the end of cultivation the lowest pH was measured in the upper layer of growing media. The highest total soluble salt level was measured in the upper layers. Upper layers accumulated more N-N03, P, Ca, and Mg. Mineral element eon tent of both media was high in all concentrations of nutrient solution. Low concentration of nutrient solution at 1.0 mS cm-1 is recommended, although Tymophylla tenuiloba can be also cultivated at higher concentrations of nutrient solution up to 2.5mS cm-l, if placed on the same bench with other bedding plants requiring more nutrients.
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Selenium is an essential element for humans, animals and some species of microorganisms. In higher plants, however, the role of selenium is still unclear. Because selenium enrichment may influence the nutrient balance of plants, a study was done to test the effects of selenite-Se (Na2SeO3⋅5H2O) on selected macronutrients content in maize (Zea mays L. var. saccharata Kcke. cv. Złota Karłowa) seedlings. Plants were grown in Hoagland I nutrient solution (pH 6,2) amended with selenite at 0 (control), 5, 25, 50 and 100 μmol⋅dm-3 for 14 days. The dry weight of the shoots was then analyzed for phosphorus (P), potassium (K), calcium (Ca) and magnesium (Mg) content. Phosphorus and calcium content increased, while potassium content decreased with increasing selenium treatments. No significant differences were found for magnesium level. Plant growth was affected by excessive selenium concentration. At low concentration (5 μmol⋅dm-3), selenium tended to stimulate the plant growth and the root elongation but at higher concentrations (50 and 100 μmol⋅dm-3) the dry mass accumulation and root tolerance index severely decreased. The study revealed that disturbances of growth and reduction of plant’s biomass at the presence of high selenium concentrations in the nutrient solution may have resulted from the disturbance of mineral balance of plants, namely accumulation of large amounts of calcium and phosphorus in shoot tissues.
This study was conducted to evaluate the effects of higher conductivity of nutrient solution created by nitrate or chloride salts of potassium and calcium on growth characteristics of pepper plants (Capsicum annuum var annuum) during four months of growth period. Two EC5 and EC8 dS/m of Hoagland nutrient solutions were prepared using various salt combinations, namely: KCl + CaCl2, KNO3 + CaNO3, and KNO3 + CaNO3 + NaCl. Hoagland nutrient solution with EC 1.8 dS/m served as a control. Higher conductivity treatments had different effects on pepper plant growth. The most significant reduction in growth parameters of plant height, shoot fresh weight, fruit yield and nutrients uptake was in plants treated with KCl + CaCl2 particularly at EC8. Application of KNO3 + CaNO3 particularly at EC5, showed no difference as compared to the control regarding many growth parameters. Application of KNO3 + CaNO3 at EC5 resulted in higher shoot fresh weight compared to the control. All salinity treatments, except from KNO3 + CaNO3 at EC5, reduced the fruit yield compared to the control. Treatments of KCl + CaCl2 and KNO3 + CaNO3 + NaCl particularly at EC8 of nutrient solution, resulted in higher leaf proline concentration, catalase and peroxidase enzymes activity compared to the control. Other conductivity treatments showed no difference in catalase or peroxidase enzymes activities. Significantly the lowest amount of leaf N, K, Mg and Ca was in KCl + CaCl2 at EC8. On the other hand, the highest leaf macronutrient concentrations were in KNO3 + CaNO3 at EC5 and/or EC8 that showed only higher leaf N and Ca values compared to the control. Leaf micronutrient concentrations were the highest in KNO3 + CaNO3 at EC5 that generally showed no difference with control plants. However, application of KCl + CaCl2 particularly at EC8 and to less extent KNO3 + CaNO3 + NaCl at EC8, reduced the leaf micronutrient concentrations. Application of KNO3 + CaNO3 at EC5 increased and KCl + CaCl2 or KNO3 + CaNO3 + NaCl at EC8 decreased the leaf Fe concentration compared to control plants.
The aim of investigations conducted in the years 2005–2007 was to determine the chemical composition of nutrient solutions in the root environment of tomato grown in wood fiber and rockwool, under the influence of diverse levels of nitrate nitrogen in the nutrient solution amounting 200, 220 and 240 mg N-NO3·dm-3. With an increase in nitrate nitrogen content in nutrient solutions used in plant fertigation a significant increase was observed in the contents of N-NO3 in nutrient solutions of the root environment, collected from wood fiber and rockwool. No such effect was found for contents of N-NH4, P, K, Ca, Mg, Fe, Mn, Zn, Cu, B, Na, Cl or pH and EC. In case of wood fiber the following nutrient concentration series was found in nutrient solutions of the root environment in relation to the nutrient solution flowing from the drippers: Na > Cu > Ca > Zn > K > Cl > B > N-NO3; the following were reduced Fe > Mg > P-PO4 > N-NH4 > Mn. Nutrients being concentrated in root environment solutions during tomato growing in rockwool were: Na > Ca > Cu > Fe > Cl > K > Zn > B > S-SO4 > N-NO3, while contents of Mg > P-PO4 > N-NH4 > Mn decreased. Despite of a wide range of carbon to nitrogen ratio (C:N) in wood fiber (123–127), no significant reduction of nitrates was shown in the root environment. It was a result of adequate application frequency of nutrient solutions during a day.
In order to determine their phytoextraction potential, four clones of Salix (1. Salix alba – clone 68/53/1; 2. Salix alba – clone 106/54/0; 3. Salix matsudana – clone SM 4041; and 4. Salix nigra – clone 0408) were exposed to elevated concentrations of Cd, Ni and Pb-EDTA in a water culture solution. The translocation ratio to upper plant parts was very low for all applied heavy metals and, therefore, the metal uptake was restricted to the roots, especially regarding Pb. The ability of the clones to extract and translocate Cd, Ni and Pb differed depending on the quantity of metal content in the nutrient solution and of the willow genotype. The ability of the investigated clones to accumulate Cd in leaves is to our knowledge among the highest so far recorded compared to other hydroponic trials in literature. The preference for Cd-stimulated root growth was determined. This genotype-specific response could be part of a mechanism for Cd resistance.
Effects of the phosphorus content in a nutrient solution (15 or 50 mg P dm-3), growing substrate (rockwool or coconut fiber) and the plant growth stage (for roots: 71 or 113 days after transplanting DAT; for leaves: 71 or 92 DAT) on the chemical composition of roots, the phosphorus content in leaves and the expression of genes encoding proteins involved in the transport of phosphorus from the medium to the plant were investigated in tomato cv. Admiro F1 grown in a foil tunnel. A fertigation system without recirculation was used. Regardless of the plant age and growing substrate, tomatoes fertilized with a nutrient solution containing 50 mg P dm-3 had more phosphorus, iron, boron and copper in roots and more phosphorus in leaves. Irrespective of the stage of plant growth and phosphorus level in the medium, the content of almost all macro- and microelements was higher in roots of plants grown in rockwool than in coconut fiber. The stage of plant growth significantly affected the mineral composition of roots as well as the P content in tomato leaves. More phosphorus was stored in roots of younger plants, whereas the phosphorus content was lower in younger than in older leaves. Our analysis of the gene expression showed that transporters encoded by LePT1-LePT4 were involved in phosphate nutrition. Expression of the genes was generally (except LePT4) higher in plants treated by the solution containing 15 mg P dm-3 than in plants treated by 50 mg P dm-3. The expression of genes LePT2, LePT3 in roots of older plants (113 DAT) was generally higher than in young plants.
The objective was the evaluation of nutrient losses to soil during horticultural plant cultivation in greenhouses where open fertigation systems are used. Losses were estimated by comparing the chemical composition of nutrient solution effluent from the drippers to the growing media with nutrient leakage from the media to the soil (called drainage or drainage waters) during soilless culture of tomato, cucumber, roses and gerbera. Largest losses were of potassium (up to 413 kg K/month/ha), nitrates (up to 231 kg NO₃-N/month/ha), calcium (up to 220 kg Ca /month/ha) and sulphur (up to 101 kg SO₄-S/month/ha). Smallest losses were of microelements (from 0.01 kg of Mn and Cu to 3.46 kg of Fe per month/ha). In Poland, the greatest horticultural pollution source for the environment is caused by the fertigation of tomato, whose cultivation area covers 2,500 ha, and whose production cycle lasts about 10 months. The least pollution arises from gerbera, which covers only about 90 ha. Sources of agricultural point pollution are generally attributed to animal farms. On the basis of the presented study the source of point pollution is not only assigned to typical agricultural production, but also to greenhouse production systems using soilless culture and open fertigation.
Chloride and sodium are the main ions contributing to soil salinity in many regions but chloride is an essential element necessary for plant growth and development. It also controls the growth of some pathogens in plants. In this experiment, the effect of the chloride ion in nutrient solution on yield and fruit quality of two strawberry cultivars: 'Selva' and 'Camarosa' grown in hydroponic culture, was studied. Three kinds of nutrient solutions were used: 1) Hoagland-Arnon solution as control; 2) Hoagland-Arnon in which potassium nitrate was replaced with potassium chloride and ammonium nitrate was added as a nitrogen source and 3) the previous medium supplemented with 1.5 mmol l-1 magnesium chloride. Plant growth, total fruit yield, fruit firmness and leaf chlorophyll content were higher in plants grown in the second solution than the others, but there was no significant difference between the three solutions in terms of single fruit weight, soluble solids content and fruit dry weight. However, using 1.5 mmol l-1 magnesium chloride in a nutrient solution also increased the height of plants as well as total fruit production. The results showed that adding the chloride ion to the nutrient solution had no negative effects on fruit quality and leaf chlorophyll content.
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