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1

Mass production of nucleopolyhedrovirus of the satin moth Leucoma salicis [LesaNPV]

100%
Ziemnicka J.
Journal of Plant Protection Research
|
2007
|
tom 47
|
nr 4
457-467
Nucleopolyhedrovirus (NPV) of the satin moth Leucoma (=Stilpnotia) salicis L. was produced by infecting the larvae with the LesaNPV strain obtained from epizootic center in Katowice. The infected larvae were reared under laboratory, greenhouse and insectarium conditions. Because L. salicis can not be reared on a semi-synthetic food, the insects were maintained on natural products. Efficiency of the mass virus production depended on an insect growth stage, virus concentration and number of infected larvae in a rearing container. The fourth-instar larvae were the best for LesaNPV replication. Inoculation of younger larval stages (third instar stadium) provided less number of inclusion bodies (insects were dying sooner and did not meet their maximum body weight). On the contrary inoculation of older stages (fifth and sixth instars) resulted in slower virus replication and low larva mortality. The virus concentration of 3 x 109 of inclusion bodies per container was the optimum inoculum for the mass virus production (double infection with the virus concentration of 1.5 x 109 inclusion bodies/1000 larvae). The larvae reared at high-density became more infected and it caused their earlier death and in consequences low virus efficiency. Rearing the insect at low density (less than 10 larvae per 1.0L container) was conducive for both an increase of insect body mass and virus replication as well. The highest number of inclusion bodies per one larva (5.3 x 109-7.7 x 109) and the highest total number of inclusion bodies (152 x 1011-188 x 1011) were achieved under these rearing conditions in a greenhouse and insectarium.
2

Cloning and partial characterization of a gene in Bombyx mori homologous to a human adiponectin receptor

100%
Zhu M., Chen K., Wang Y., Guo Z., Yin H., Yao Q., Chen H.
Acta Biochimica Polonica
|
2008
|
tom 55
|
nr 2
241-249
In this study, we report the cloning and characteristics of an adiponectin-like receptor gene from Bombyx mori (BmAdipoR) with highly conserved deduced amino-acid sequences and similar structure to the human adiponectin receptor (AdipoR). Structural analysis of the translated cDNA suggested it encoded a membrane protein with seven transmembrane domains. BmAdipoR was found to be expressed in multiple tissues and highly expressed in Malpighian tubules, fat body and testis. BmNPV (Bombyx morinucleopolyhedrovirus) bacmid system combined with confocal microscopy revealed that BmAdipoR was targeted to the cell membrane. We also found that infection with BmNPV did not have an effect on BmAdipoR mRNA quantity in the midgut of susceptible Bombyx moristrain (306) at 48 h, but BmAdipoR mRNA quantity increased significantly at 72 h. We concluded that BmAdipoR gene was a membrane protein ubiquitously expressed in Bombyx moritissues and that its expression was altered by treating with BmNPV.
3

The first record of nucleopolyhedrovirus isolated from the satin moth Leucoma salicis L. [Lepidoptera, Lymantriidae] in Turkey

100%
Yaman M.
Folia Biologica
|
2008
|
tom 56
|
nr 3-4
273-276
4

Outbreaks and natural viral epizootics of the satin moth Leucoma salicis L. [Lepidoptera: Lymantriidae]

80%
Ziemnicka J.
Journal of Plant Protection Research
|
2008
|
tom 48
|
nr 1
23-39
Long standing systematic observations on Leucoma salicis populations revealed numerous occurrences of outbreaks and natural viral epizootics. Number of insects appearing in outbreaks at peak density ranged from 450 to 3250 on 10 examined poplar trees (up to 2 m height). Abundance of virus within population of such a high density increased along with an increase of insect population. Populations with high density and high level of nucleopolyhedrovirus (LesaMNPV) and cypovirus did not last long and collapsed suddenly. Outbreaks of the satin moth were favored by warm and humid conditions while warm and dry spring, summer months were conducive to viral epizootic. Most outbreaks happened in the years with mean temperatures of spring and summer months above 15°C and 50-60% RH while, most epizootics were recorded at similar temperature conditions but lower RH, i.e. 40-50%.
5

Effects of viral epizootic induction in population of the satin moth Leucoma salicis L. [Lepidoptera: Lymantriidae]

80%
Ziemnicka J.
Journal of Plant Protection Research
|
2008
|
tom 48
|
nr 1
41-52
An epizootic was induced by introduction of Leucoma salicis nucleopolyhedrovirus (LesaMNPV) into satin moth (L. salicis) population feeding on poplar Populus nigra L. Introduction of LesaMNPV virus at a rate 4 x 102 of inclusion bodies per tree into insect population (stage L3 and L4) resulted in a rapid outbreak collapse both in the peak and early decline phases. A rate of epizootic development depended on healthiness of satin moth larvae before the treatment. Insect population with a high level of nucleopolyhedrovirus (app. 21% and 26% infected insects) reached the epizootic peak on the 18th day after the treatment (85% and 86% infected insects). Induced epizootic resulted in the decline phase of L. salicis population that continued for subsequent 6 years. Accidental occurrence of fungus Beauveria bassiana (Bals.) Vuill. extended the population decline phase for the period of at least 8 years. Induced epizootic caused a rapid collapse of satin moth outbreak with direct transition from the population peak phase into the population collapse phase with omission of the decline phase. This pattern was not observed in not treated populations. Artificially induced epizootics affected healthiness of insect pupae and adults as well the reproductive potential of females and healthiness of offspring. The weight of pupae obtained from treated larvae was lower when compared with the control. Virus infections were found more frequently in female pupae than in male pupae. This resulted in a 6-28 fold decrease of the number of egg masses and 3.5-5 times lower numbers of eggs deposited by females. Offspring of infected pairs showed symptoms of viral infection (15-28% infected larvae) and the number of offspring was 70-800 times lower in comparison with offspring of not infected pairs. This study presents the results of induced epizootic and has been the first attempt to review and sum up results of long-term research on evaluation of nucleopolyhedrovirus efficacy in natural conditions.
6

Characterization of a nucleopolyhedrovirus isolated from the laboratory rearing of the beet armyworm Spodoptera exigua [Hbn.] in Poland

80%
Jakubowska A., Vlak J. M., Ziemnicka J.
Journal of Plant Protection Research
|
2005
|
tom 45
|
nr 4
279-286
A nucleopolyhedrovirus isolated from the beet armyworm Spodoptera exigua (Polish laboratory culture), SeMNPV (P), morphologically similar to the viral bioinsecticide virus Spod-XR , was characterized molecularly and biologically. Phylogenetic analysis based on three conserved baculovirus genes, polh, lef-8 and pif-2, showed the highest homology of SeMNPV (P) to Mamestra brassicae (Mb) MNPV and M. configurata (Maco) MNPV, and much less to SeMNPV (Spod-XR). These findings were confirmed by genomic DNA restriction profile analyses. Bioassays revealed that SeMNPV isolated from the commercial bioinsecticide Spod-XR was themost infectious for S. exigua, while the infectivity of SeMNPV (P) and MbMNPV was significantly lower. These data suggest that SeMNPV (P) is a variant of MbMNPV.
7

A Turkish isolate of Neodiprion sertifer nucleopolyhedrovirus [NeseSNPV]

80%
Yaman M.
Bulletin of the Polish Academy of Sciences. Biological Sciences
|
2003
|
tom 51
|
nr 4
8

Expression of Bombyx mori nucleopolyhedrovirus ORF76 in permissive and non-permissive cell lines by a novel Bac-to-Bac-BmNPV baculovirus expression system

80%
Su W. J., Wu Y., Wu H.-L., Zhu S.-Y., Wang W.-B.
Polish Journal of Microbiology
|
2008
|
tom 57
|
nr 4
Open reading frame 76 of Bombyx mori nucleopolyhedrovirus (BmNPV), designated as Bm76, is a gene whose function is completely unknown. With EGFP fused to the 3' terminal of Bm76 as the reporter gene and BmNPV bacmid as the expression vector, a recombinant bacmid was successfully constructed expressing Bm76-EGFP fusion protein under the control of polyhedrin promoter in Bombyx mori cells (Bm cells), BmNPV's permissive cell line, laying the foundation for rescue experiment of Bm76 deletion mutant. Moreover, the supernatant from Bm cells transfected with the recombinant bacmid was used to infect Trichoplusia Ni cells (Tn cells), BmNPV's non-permissive cell line. Unexpectedly, the expression of Bm76-EGFP fusion protein in some Tn cells was detected, implying that viral DNA was replicated in these cells. The causes are being studied for the inability of BmNPV to produce enough viable budded viruses in Tn cells despite of viral DNA replication.
9

Interakcje nukleopoliedrowirusa AgseNPV i preparatu chemicznego Nurelle D 550 EC u rolnicy zbozowki Agrotis segetum [Den. et Schiff.]

71%
Ziemnicka J.
Progress in Plant Protection
|
2008
|
tom 48
|
nr 4
1340-1344
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