Wyniki wyszukiwania

1

Methyl-CPG-binding protein 2 [MECP2]: role in development and gene expression

100%

Stratling W. H., Yu F., Thiesen J., Buschdorf J.

Cellular and Molecular Biology Letters

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2000

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tom 05

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nr 2

2

Comparison of nuclear protein SH-DNA ratio in species differing in 2C DNA content and dynamics of DNA endoreplication

100%

Marciniak K.

Folia Histochemica et Cytobiologica

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1993

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tom 31

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nr 1

3

Tissue specific interactions of the nuclear proteins with the bovine tyrosine hydroxylase gene

100%

Lenartowski R., Pluskota W., Wojciechowski W., Goc A.

Cellular and Molecular Biology Letters

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1997

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tom 02

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nr 2

4

Neoplastic-associated nuclear proteins in colorectal cancer

88%

Kilianska Z., Szymczyk P., Wojcik M., Brys M., Jakubik J., Berner A., Hanausek-Walaszek M., Berner J., Krajewska W. M.

Cellular and Molecular Biology Letters

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1997

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tom 02

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nr 2

5

Analysis of the low molecular weight nuclear polypeptide by isoelectrofocusing of nuclear proteins first separated by gel electrophoresis in SDS

88%

Borowicz B., Domaniewski J.

Archivum Veterinarium Polonicum

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1994

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tom 34

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nr 3-4

6

Alterations in cell nuclei during apoptosis

75%

Rogalinska M.

Cellular and Molecular Biology Letters

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2002

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tom 07

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nr 4

Apoptosis is a genetically programmed phenomenon that aids in maintaining homeostasis in multicellular organisms. The characteristic morphological features of apoptosis are highly conservative and are dependent on the cell type and the apoptotic inducer. The nuclear events occurring during apoptosis include changes at the molecular level (i.e. DNA cleavage, modifications of nuclear polypeptides, and proteolysis of several proteins important for cell maintenance), and, consequently, alterations at the morphological level (i.e. chromatin condensation, nuclear shrinkage, DNA fragmentation and apoptotic body formation). These events are still not fully understood. It is very probable that a progressive decrease in pH could also be an essential factor for the induction of nuclease and protease activities, and an important element of the optimal conditions for their function. This review details the current state of knowledge on apoptotic nuclear events, with particular focus on the proteins involved in the execution of apoptosis in cell nuclei, and on the differences in substrate cleavage profiles for different types of cell undergoing celi death.

7

Novel mechanisms of neurogenesis and neural repair

75%

Gotz M.

Acta Neurobiologiae Experimentalis

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2019

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tom 79

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nr Suppl.1

We study the mechanisms of neurogenesis in order to implement them for neuronal repair. I will present unpublished work about the molecular function of Trnp1, a novel nuclear protein, with key roles in promoting neural stem cell self‑renewal and neurogenesis. Trnp1 shows unprecedented functions in regulating several nuclear processes by its N-terminal intrinsically disordered region, which is highly conserved in mammals. I will then show that Trnp1 is also critical for direct neuronal reprogramming and provide an update on the recent breakthrough in direct glia-to-neuron conversion after brain injury. I will then move on to discuss the integration of replaced neurons into the circuitry of the murine cerebral cortex – that normally does not integrate new neurons at adult stages – and present unpublished data about the mechanisms regulating this integration. Taken together, our knowledge about basic mechanisms of neurogenesis allows us to make great strides towards neuronal repair.

8

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Identification and characterization of S-nitrosylated nuclear proteins in Arabidopsis thaliana

75%

Chaki M., Shekariesfahlan A., Lindermayr C.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2013

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tom 94

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nr 2

9

Cross-linking of nuclear proteins to DNA by platinum compounds

75%

Walter Z., Wozniak K., Kowalik J.

Cellular and Molecular Biology Letters

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1997

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tom 02

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nr 4

In this work we investigated the lymphocyte nuclear proteins which participated in DNA-protein cross-links induced by three platinum compounds: cis-DDP, trans-DDP and carboplatin. Our studies indicated that many proteins were cross- linked to DNA in the intact cell. Trans-DDP was the most effective cross-linker. The cross-linking process depended on time of the cells incubation with aforementioned compounds. Carboplatin produced the DNA-protein cross-links more slowly and less effectively than cis-DDP. After incubation of the cells with cis-DDP, trans- DDP and carboplatin a similar protein pattern was obtained. In case of all compounds studied 34.5 kDa protein was abundantly represented.

10

Novel regulators of photosynthesis

63%

Leister D.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2013

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tom 94

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nr 2

11

Multiple extraction method for DNA, RNA and nuclear protein isolation from peripheral blood

63%

Ferry A. E., Pace B. S.

Cellular and Molecular Biology Letters

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1998

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tom 03

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nr 4

Obtaining adequate samples for nucleic acid or protein analysis from a limited number of cells can be a difficult task. The steps for isolation of DNA, cytoplasmic RNA and nuclear proteins from mononuclear cells collected from a single peripheral blood sample are outlined below. A previously described technique for rapid isolation of nuclear proteins was modified to acquire RNA and DNA of sufficient quantity and quality to perform analyses at the molecular level without altering the quality of protein extracted. This approach is applicable for use with peripheral blood mononuclear cells, primary cultures and immortalized cell lines.

12

In search for effective modulators of the proteasome activity

51%

Karpowicz P., Stoj J., Gaczynska M., Osmulski P., Jankowska E.

Acta Neurobiologiae Experimentalis

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2013

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tom 73

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nr 1

Proteasome is a multi-activity enzyme involved in a ubiquitin-dependent turnover of cytoplasmic and nuclear proteins. It recognizes and digests short-lived regulatory proteins, influencing cellular processes as crucial as progression of the cell cycle, transcription, oncogenesis and flux of substrates through metabolic pathways. The enzyme is responsible also for the housekeeping chores, degrading misfolded or oxidatively damaged proteins. Defects in the proteasome action play a causal role in development of a number of diseases, among which are cerebral ischemia and neurodegenerative disorders such as Huntington’s, Alzheimer’s, and Parkinson’s diseases. Being a multifunctional proteolytic machinery, the proteasome must act under a strict control to prevent massive degradation of all intracellular proteins, which would result in a cell death. One of the levels of such a control is the proteasome structure itself. The core particle called 20S proteasome is a barrel-like structure made up of four rings of seven subunits each. The outer (α) rings play predominantly a structural role forming a kind of a gated channel leading to the proteolytic chamber. The inner-β-rings harbor six active sites, concealed inside the cavity formed by the β subunits. So far, the only proteasome-targeting agents used in clinics are competitive inhibitors, directly blocking the enzyme’s active sites. However, the multi-subunit barrel-like structure of the 20S proteasome encourages to test compounds which can target allosteric interactions between subunits and influence the gating mechanism, involved in the control of the substrates’ uptake. Such modulators may provide a precise and substrate-specific regulation of the proteasome catalytic performance. Additionally, targeting the allosteric interactions may enable not only inhibition but also stimulation of the proteasome, which is crucial in managing disorders connected with the proteasome not sufficient activity, such as neurodegenerative diseases. A variety of protein ligands, interacting with the outer ring of the 20S proteasome and modulating its activity, is already known. They can serve as templates for design of putative small-molecule allosteric drugs. In an effort to find synthetic compounds able to enhance or suppress the performance of the proteasome active centers we utilize one of such protein ligands – HIV-1 Tat protein. The protein is known to inhibit the core proteasome and to interfere with the physiological PA28 activator in its binding to the 20S. G48RKKRRQRRRPS59 fragment of HIV-1 Tat (Tat1) occurred to be very efficient in the 20S proteasome inhibition. By single and multiple alanine substitutions we have recognized “hot spots” in the sequence of Tat1. NMR and molecular dynamics calculations allowed us to correlate these putative pharmacophores with the structural turns. By introduction of a non-peptide turn-inducing modification to the Tat1 sequence we have obtained the derivatives highly toxic for human cultured cancer cells HeLa.S3. The work was supported by grants: NCN 2011/01/B/ST5/06616 and DS/8440-4-0172-2

13

Abscisic acid does not influence the subcellular distribution of the HYL1 protein from Arabidopsis thaliana

51%

Lesicka-Gorecka J., Szarzynska B., Sawczak M., Bagdiul I., Gorski P., Jarmolowski A., Szweykowska-Kulinska Z.

Acta Biochimica Polonica

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2008

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tom 55

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nr 3

517-524

HYL1 is a nuclear protein involved in the processing of miRNAs but its exact function remains unknown. Arabidopsis thalianahyl1mutants exhibit hypersensitivity to ABA. We decided to answer the question whether ABA affects the HYL1 protein localization within the cell and show that it does not. We also studied the expression of HYL1 in different tissues and organs. In this paper we show for the first time the expression profile of the HYL1 protein using anti-HYL1 antibodies. The protein is present in seedlings and mature plants in all organs studied, with the highest amount in inflorescences. A. thalianaHYL1 protein has several repetitions of a 28-amino-acid sequence at the C-terminus that confer protein instability. Our bioinformatic analysis of HYL1 homologs in different Brassicaspecies shows that this repetition is typical only for Arabidopsis. This may suggest a relatively late evolutionary acquisition of the C-terminal domain.

14

Induction of DNA breakage in X-irradiated nucleoids selectively stripped of nuclear proteins in two mouse lymphoma cell lines differing in radiosensitivity

51%

Kruszewski M., Iwanenko T.

Acta Biochimica Polonica

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1998

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tom 45

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nr 3

The role of nuclear proteins in protection of DNA against ionizing radiation and their contribution to the radiation sensitivity was examined by an alkaline version of comet assay in two L5178Y (LY) mouse lymphoma cell lines differing in sensitivity to ionizing radiation. LY-S cells are twice more sensitive to ionizing radiation than LY-R cells (D0 values of survival curves are 0.5 Gy and 1 Gy, respectively). Sequential removal of nuclear proteins by extraction with NaCl of different concentrations increased the X-ray induced DNA damage in LY-R nucleoids. In contrast, in the radiation sensitive LY-S cell line, depletion of nuclear proteins practically did not affect DNA damage. Although there is no doubt that the main cause of LY-S cells' sensitivity to ionizing radiation is a defect in the repair of double-strand breaks, our data support the concept that nuclear matrix organisation may contribute to the cellular susceptibility to DNA damaging agents.

15

Changes in the relative content of nuclear, nucleolar and cytoplasmic proteins to DNA in species with different DNA endoreplication dynamics. I. Endosperm

38%

Marciniak K.

Bulletin of the Polish Academy of Sciences. Biological Sciences

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1993

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tom 41

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nr 3

Ograniczanie wyników

Methyl-CPG-binding protein 2 [MECP2]: role in development and gene expression

Comparison of nuclear protein SH-DNA ratio in species differing in 2C DNA content and dynamics of DNA endoreplication

Tissue specific interactions of the nuclear proteins with the bovine tyrosine hydroxylase gene

Neoplastic-associated nuclear proteins in colorectal cancer

Analysis of the low molecular weight nuclear polypeptide by isoelectrofocusing of nuclear proteins first separated by gel electrophoresis in SDS

Alterations in cell nuclei during apoptosis

Novel mechanisms of neurogenesis and neural repair

Identification and characterization of S-nitrosylated nuclear proteins in Arabidopsis thaliana

Cross-linking of nuclear proteins to DNA by platinum compounds

Novel regulators of photosynthesis

Multiple extraction method for DNA, RNA and nuclear protein isolation from peripheral blood

In search for effective modulators of the proteasome activity

Abscisic acid does not influence the subcellular distribution of the HYL1 protein from Arabidopsis thaliana

Induction of DNA breakage in X-irradiated nucleoids selectively stripped of nuclear proteins in two mouse lymphoma cell lines differing in radiosensitivity

Changes in the relative content of nuclear, nucleolar and cytoplasmic proteins to DNA in species with different DNA endoreplication dynamics. I. Endosperm