The developement of DNA microarray technique has opened the possibility of large-scale studies on genomic determinants of growth and maliganancy of neoplasms. It enables a wide analysis of cancer gene expression in a short period of time. Such defined and specific profiles of gene expression are a molecular signature (“molecular portrait”), which in the future could be used as a reliable marker of cancer malignancy and prognosis. Molecular identification of breast cancer sub-types and their relation with prognosis was one of the most important microarray studies. Unfortunately, in contrast to medical oncogenomics, the development of veterinary oncogenomics is not so dynamic. The first oncogenomic studies on canine mammary gland tumor cell lines were performed at Utrecht University. They successfully identified deregulated pathways pertaining to cell line phenotype similar to the published human breast cancer data sets. The results presented in this paper show that high proliferative and anti-apoptotic potential of canine mammary gland cancer cells may be associated with an enhanced expression of genes involved in the Ca²⁺ signaling pathway (clamodulin 1, 2 and 3, and ryanodine receptor) and growth hormone cellular pathway. Moreover, a comparative study of gene expression in canine mammary tumor tissue and primary cell lines derived from those tumors was conducted. The obtained results show that the transcriptional profile of the primary cell culture resembles that in tumor tissue, and prove that cell cultures can be used as a reliable model for oncogenomic and pharmacogenomic studies. These promising results make it possible to anticipate a progressive development of veterinary oncogenomics.
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.