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This paper deals with normal values of some serum biochemical parameters in broiler chickens with regard to their age and sex. Studies were carried out every week on clinically healthy broiler chickens of the Vedetta race, both females and males aged 2-7 weeks. In blood serum collected from the birds, levels of total protein, glucose, total cholesterol, triglycerides, calcium (Ca) and inorganic phosphorus (P) as well as the activity of some enzymes including alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (AP), lactate dehydrogenase (LDH), creatine kinase (CK), cholinesterase (ChE) were determined. No differences in the analysed biochemical index values between the females and the males were found. The following indices were found to be age dependent: levels of total protein (increased from 5 weeks of age), triglycerides and AP activity (decreasing with age), CK activity (increased in females from 5 and in males from 6 weeks of age), ChE activity (decreased in females from 6 and in males from 5 weeks of age).
The aim of the study was to develop an intravital method of collecting hemolymph and to establish standards for the biochemical parameters of the hemolymph of the Cornu aspersum snail species. The research material consisted of 80 Cornu aspersum maxima snails. Hemolymph was collected from the main vessel with a sterile syringe and needle after removing a fragment of the shell. Hemolymph was studied biochemically by the colorimetric method. The parameters evaluated were aspartate transaminase (AST), alanine transaminase (ALT), amylase (AMYL), urea (UREA), and triglycerides (TG). An average of 0.54 ml hemolymph was collected from the animals tested. After 24 hours, a film formed in place of the removed shell fragment and it underwent mineralization within a few days. All animals included in the study survived, and from some of them it was possible to collect hemolymph several times at intervals of several days. The proposed method of hemolymph collection made it possible to obtain material to develop standards for the biochemical parameters of hemolymph and consequently to monitor the health status of these mollusks.
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