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Soil subject to a three-year pot experiment was analyzed, in which the following factors were considered: (i) soil contamination with nickel (0, 75, 150 and 225 mg Ni kg⁻¹ of soil); (ii) liming (0 Ca and Ca in a dose calculated according to 1 Hh of soil); (iii) organic fertilization (with no organic fertilization, rye straw and brown coal from the Turów coalmine). The test plant was orchardgrass, which was harvested in the amount of four cuts each year of the experiment. The copper and zinc fractions were assessed using the BCR method, whereas the pH was calculated using the potentiometer method. The total content of copper and zinc in the analyzed soil did not exceed the values of the geochemical background of the soils of Poland. No impact of the varied content of nickel in the soil on the total content and distribution of Cu and Zn was found in the investigated fractions. Liming caused an increase in the total content of zinc in the analyzed soil and a decrease in the content of Cu and Zn in the F1 fraction. The straw and brown coal caused an increase in the content of both metals in the soil in the F3 fraction.
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Nickel in the environment

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The importance of nickel (Ni) in the environment is an issue that is gaining broader recognition. While nickel is an element essential for plants, it is also a heavy metal. Nickel is a component of nine metalloenzymes, including urease, which participates in urea hydrolysis. It also helps some plants to protect themselves against pathogens and herbivorous insects. There are many sources of Ni in the environment, which can be a problem because at higher concentrations this element is toxic to plants and other living organisms. Therefore, standards have been defined for the Ni content in air, water, soil and plants. Its content is monitored in the air. More and more frequently, attention is paid to this element as an allergen in humans. In the world, attempts have been made to phytoextract nickel from contaminated soils using nickelphilous plants, the so-called hyperaccumulators, and even to recover the metal from these plants by so-called phytomining. On the other hand, nickel-containing fertilizers are marketed and used in cases of nickel deficiency in plants. In industry, this element is primarily used for the production of steel and alloys. The most recent application of nickel is related to graphene, which was invented 10 years ago. Although nickel contamination does not occur in Poland, we cannot rule out this risk in the future. Thus, it is important to monitor the fate of nickel in the environment.
Both urea and urease were subjects of early scientific investigations. Urea was the first organic molecule to be synthesized and jack bean urease was the first enzyme ever to be crystallized. About 50 years later it was shown to be the first nickel metalloenzyme. Since then, nickel-dependent ureases have been isolated from many bacteria, fungi and higher plants. They have similar structures and mechanisms of catalysis. A urease apoenzyme needs to be activated. This process requires participation of several accessory proteins that incorporate nickel into the urease forming catalytic site. In this review, ureases from various organisms are briefly described and the similarities of their structures discussed. Moreover, the significance of urea recycling in plants is explained and recent literature data about the function and activation of plant ureases are presented.
The role of contact with nickel-containing coins has been controversial. The aim of our study was to compare the release of nickel from Euro (1 and 2) coins and from Polish coins (2 PLN and 5 PLN) at 4ºC and 32ºC in an immersion test using artificial sweat according to the EU reference method. Nickel extract was analyzed at 8 time points starting from 1 h up to 168 h. After 7 days of test duration at 32ºC, nickel ion concentration was 96.27±4.01 μg/cm2, 79.31±1.95 μg/cm2, 38.34±1.19 μg/cm2, and 32.17±2.36 μg/cm2 for 2 Euro, 1 Euro, 5 PLN, and 2 PLN, respectively. The amount of nickel ion released at 4ºC was reduced by about 70% and 40% for Euro and Polish coins, respectively. These values exceed the limit acceptable for prolonged contact with human skin as defined by the EU Nickel Directive, which indicates that nickel may be capable of eliciting allergic reactions in subjects handling nickel-containing coins daily.
Substances present in soils are of natural or anthropogenic origin. As a result of industrial activity, large quantities of potentially hazardous elements such as heavy metals are deposited in the environment. They may occur as unavailable (similar to the total form), or available for plants. The content of nickel in the soil from the former sanitary zone of the Głogów Copper Smelter does not exceed the Polish threshold values for industrial areas; however, its content may be influenced by other factors such as content of organic matter and soil reaction. In this article we present the results of the nickel pollution in the former sanitary zone of the Głogów Copper Smelter.
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Enzymatic activity of nickel-contaminated soil

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A pot experiment was performed to determine the effect of soil contamination with nickel, applied at a dose of 100, 200, 300 and 400 mg kg-1, on the activity of dehydrogenases, urease, acid phosphatase and alkaline phosphatase. The impact of nickel on the enzymatic activity of soil was studied on samples of heavy loamy sand and light silty loam. The experiment was conducted in two series: in the first one soil was cropped to yellow lupine, and in the second one it was left uncropped. Soil samples were analyzed on day 14, 28, 42 and 56. It was found that soil contamination with nickel reduced the activity of all the enzymes. This negative influence was most noticeable in the case of dehydrogenase. The activity of urease and alkaline phosphatase was higher in light silty loam, while the activity of dehydrogenases and acid phosphatase was higher in heavy loamy sand. The activity of dehydrogenases and urease was higher in soil cropped to yellow lupine, whereas the activity of acid phosphatase and alkaline phosphatase was higher in uncropped soil. Yellow lupine was sensitive to excessive amounts of nickel in the soil, and partly alleviated the adverse impact of this heavy metal on urease activity, but did not reduce its inhibitory effect on the other enzymes.
This paper presents the occurrence, properties and origin of chromium, nickel and cobalt in environmental samples - soil, water and the atmosphere. Methods of determining the above-mentioned elements, as well as the ways of preparing environmental samples for chemical analyses were presented. The content of chromium, nickel and cobalt in the atmosphere, the naturally occurring waters and soil was shown, along with the permissible concentrations of the above elements in the three divisions of the environment legally required in Poland and other countries in the world.
An analysis of industrial wastes is a difficult and complicated procedure because of their complex contents which depend on the nature of production and technology being used. The present paper gives selected analytical problems concerning the determination of nickel and iron in wastes coming from the metalurgical industry. It has been concluded that the methods of mineralizing wastes need to be adjusted to their complexity. For emulsion wastes the most suitable method is microwave mineralization.
Nickel tolerant callus lines of Setaria italica L. were developed from callus cultures grown on MS medium supplemented with 0.5 mg·dm⁻³ kinetin+2.0 mg·dm⁻³ 2,4-D+2.0 mg·dm⁻³ Ni⁺². Standard growth parameters such as callus fresh and dry weight, growth tolerance index were used as indicators of nickel toxicity. Measurements as early as 2 weeks after the beginning of the treatments did not yield consistent results. However, growth tolerance index at 4, and 8 weeks after the beginning of treatments yielded significant differences among the non-tolerant and tolerant calli. The tolerant calli has enhanced growth at 2.0 mg·dm⁻³ Ni⁺² while non-tolerant calli showed a reverse trend in growth in the presence of 2.0–2.5 mg·dm⁻³ of nickel. The tolerant calli differentiated into mass of embryogenic calli within 4 weeks of culture which could be maintained for prolonged period without loss of regenerative capacity.
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