Preferencje help
Polish version English version Język
Widoczny [Schowaj] Abstrakt
Liczba wyników

Znaleziono wyników: 15

Liczba wyników na stronie
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników

Wyniki wyszukiwania

Wyszukiwano:
w słowach kluczowych:  neurogenesis
help Sortuj według:

help Ogranicz wyniki do:
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
1

Novel mechanisms of neurogenesis and neural repair

100%
Gotz M.
Acta Neurobiologiae Experimentalis
|
2019
|
tom 79
|
nr Suppl.1
We study the mechanisms of neurogenesis in order to implement them for neuronal repair. I will present unpublished work about the molecular function of Trnp1, a novel nuclear protein, with key roles in promoting neural stem cell self‑renewal and neurogenesis. Trnp1 shows unprecedented functions in regulating several nuclear processes by its N-terminal intrinsically disordered region, which is highly conserved in mammals. I will then show that Trnp1 is also critical for direct neuronal reprogramming and provide an update on the recent breakthrough in direct glia-to-neuron conversion after brain injury. I will then move on to discuss the integration of replaced neurons into the circuitry of the murine cerebral cortex – that normally does not integrate new neurons at adult stages – and present unpublished data about the mechanisms regulating this integration. Taken together, our knowledge about basic mechanisms of neurogenesis allows us to make great strides towards neuronal repair.
2

Genetic and molecular mechanisms in neurogenesis

100%
Sura P., Srebro Z.
Acta Biologica Cracoviensia. Series Zoologia
|
2004
|
tom 46
3

Genomic imprinting and the epigenetic regulation of adult neurogenesis

84%
Ferron S., Kleine I., Ferguson-Smith A. C.
Acta Neurobiologiae Experimentalis
|
2013
|
tom 73
|
nr 1
Genomic imprinting is a normal process causing genes to be expressed from only one of the two parental chromosome homologues according to their parental origin. Imprinted genes function in a range of developmental processes. In recent years, data has emerged indicating discordance of imprinting between mouse and man, polymorphic imprinting between different individuals and tissue-specific imprinting within individuals. This suggests that imprinting might be an adaptable and dynamic process with the potential to act as a mechanism regulating gene dosage in different developmental contexts. Delta-like homologue 1 (Dlk1) is a paternally expressed imprinted gene that encodes both a transmembrane protein and a secreted isoform generated by alternative splicing, and is an atypical member of the Notch/Delta/Serrate family of developmental signalling molecules. Although widely expressed during embryonic development, only a few tissues including neurogenic regions of the brain retain Dlk1 expression in adults. Analysis of neurogenesis in the SVZ of Dlk1 mutant mice shows a reduction in the numbers of stem cells in vivo and an impairment of newborn neurons incorporated into the olfactory bulb as well as fewer primary neurospheres in vitro suggesting that normal levels of Dlk1 are necessary for the life-long maintenance of neural stem cells (NSCs). Within the SVZ, DLK1 is a niche factor secreted by astrocytes and that membrane-bound DLK1 is required in NSCs to respond to it. In contrast to the neighbouring Gtl2 gene, we observe specific absence of Dlk1 imprinting in the stem cell and astrocyte populations in the SVZ niche indicating that the mechanism conferring biallelic expression can override the imprint selectively at Dlk1 to control normal neurogenesis in the adult brain. This neurogenic requirement for both the maternally and paternally expressed alleles of the canonically imprinted Dlk1 gene supports the hypothesis that control of gene dosage by absence of imprinting is an important developmental process. We are testing the hypothesis that other imprinted genes important in neurogenesis may also modulate imprinting to control gene dosage.
4

Role of ubiquitin ligases in neural stem and progenitor cells

84%
Naujokat C.
Archivum Immunologiae et Therapiae Experimentalis
|
2009
|
tom 57
|
nr 3
177-188
5

Serotonin and neurogenesis in the hippocampal dentate gyrus of adult mammals

84%
Djavadian R. L.
Acta Neurobiologiae Experimentalis
|
2004
|
tom 64
|
nr 2
6

Multipotent stem cells in the adult mammalian central nervous system

84%
Wozniak W.
Folia Morphologica. Supplement
|
1999
|
tom 58
|
nr 2
7

The classification of microglial activation phenotypes on neurodegeneration and regeneration in Alzheimer’s disease brain

67%
Varnum M. M., Ikezu T.
Archivum Immunologiae et Therapiae Experimentalis
|
2012
|
tom 60
|
nr 4
8

Reduction of the number of new cells reaching olfactory bulbs impairs olfactory perception in the adult opossum

67%
Grabiec M., Turlejski K., Djavadian R.
Acta Neurobiologiae Experimentalis
|
2009
|
tom 69
|
nr 2
168-176
In adult mammals cells generated in the subventricular zone (SVZ) migrate to olfactory bulbs (OB). Functional significance of this continuous neurogenesis is not clear. We injected opossums (Monodelphis domestica) for seven consecutive days with a 5HT1A agonist (8-OH-DPAT or buspirone), or its antagonist WAY100635. One hour after each of these injections bromodeoxyuridine (BrdU), a marker of dividing cells was also injected. Two months later, when newly generated neurons settled in the OB and matured the ability of these opossums to detect hidden food by olfactory cues was tested. Afterwards, numbers of BrdU-labeled cell nuclei in their OB were counted and a phenotype of labeled cells established. In all groups investigated the majority of new cells differentiated into neurons (55-76%) and a lower proportion into astroglia (6-12%). Numbers of BrdU-labeled cells differed depending on the applied treatment: both agonists of the 5HT1A receptor increased these numbers, while its antagonist decreased them. The increased number of new OB interneurons did not change the time required for finding all three food items and therefore did not improve the opossums' performance in this test of the olfactory perception. However, opossums that had the reduced number of new generated OB cells searched longer for each food item and in consequence took three times longer to find all three crickets, than did opossums from other groups. In conclusion, lower numbers of new neurons in the opossums OB correlated with their worse behavioral performance in a test based on olfactory perception.
9
Content available remote

Stem cells for neural regeneration - a potential application of very small embryonic-like stem cells

67%
Ratajczak J., Zuba-Surma E., Paczkowska E., Kucia M., Nowacki P., Ratajczak M. Z.
Journal of Physiology and Pharmacology
|
2011
|
tom 62
|
nr 1
The goal of regenerative medicine is to ameliorate irreversible destruction of brain tissue by harnessing the power of stem cells in the process of neurogenesis. Several types of stem cells, including mesenchymal stem cells, hematopoietic stem cells, as well as neural cells differentiated from embryonic stem cell lines, have been proposed as potential therapeutic vehicles. In this review paper we will discuss a perspective of stem cell therapies for neurological disorders with special emphasis on potential application of cells isolated from adult tissues. In support of this our group found that murine bone marrow contains a mobile population of Oct-4+CXCR4+SSEA-1+Sca-1+lin–CD45– very small embryonic-like stem cells (VSELs) that are mobilized into peripheral blood in a murine stroke model. The number of these cells in circulation increases also after pharmacological mobilization by administration of granulocyte colony stimulating factor (G-CSF). Recently we found that VSELs are present in various non-hematopoietic adult organs and, interestingly, our data indicate that the brain contains a high number of cells that display the VSEL phenotype. Based on our published data both in human and mice we postulate that VSELs are a mobile population of epiblast/germ line-derived stem cells and play an important role as an organ-residing reserve population of pluripotent stem cells that give rise to stem cells committed to particular organs and tissues - including neural tissue. In conclusion human VSELs could be potentially harnessed in regenerative medicine as a source of stem cells for neurogenesis.
10

Developmental expression of P5 ATPase mRNA in the mouse

67%
Weingarten L. S., Dave H., Li H., Crawford D. A.
Cellular and Molecular Biology Letters
|
2012
|
tom 17
|
nr 1
P5 ATPases (ATP13A1 through ATP13A5) are found in all eukaryotes. They are currently poorly characterized and have unknown substrate specificity. Recent evidence has linked two P5 ATPases to diseases of the nervous system, suggesting possible importance of these proteins within the nervous system. In this study we determined the relative expression of mouse P5 ATPases in development using quantitative real time PCR. We have shown that ATP13A1 and ATP13A2 were both expressed similarly during development, with the highest expression levels at the peak of neurogenesis. ATP13A3 was expressed highly during organogenesis with one of its isoforms playing a more predominant role during the period of neuronal development. ATP13A5 was expressed most highly in the adult mouse brain. We also assessed the expression of these genes in various regions of the adult mouse brain. ATP13A1 to ATP13A4 were expressed differentially in the cerebral cortex, hippocampus, brainstem and cerebellum while levels of ATP13A5 were fairly constant between these brain regions. Moreover, we demonstrated expression of the ATP13A4 protein in the corresponding brain regions using immunohistochemistry. In summary, this study furthers our knowledge of P5-type ATPases and their potentially important role in the nervous system.
11

Oxygen-glucose deprivation (OGD) promotes microglia activation and gliogenesis but not neurogenesis in organotypic hippocampal slice culture (OHC)

67%
Ziemka-Nalecz M., Wojcik-Stanaszek L., Zajac H., Zalewska T.
Acta Neurobiologiae Experimentalis
|
2013
|
tom 73
|
nr 1
Organotypic hippocampal cultures are used as an alternative model for studying molecular mechanism(s) of neurogenesis after combined oxygen-glucose deprivation (OGD) mimicking ischemic conditions. The aim of the present work was to investigate the effect of OGD on stem/progenitor cells proliferation and/or differentiation in the hippocampus. Our attention was primarily focused on the relationship between neurogenesis-associated processes and activity of matrix metalloproteinases (MMPs). Cell proliferation was detected by using BrdU incorporation. Newly generated BrdU (+) cells were identified by labeling with specific cell markers. In order to check the activity and localization of MMPs we conducted in situ zymography in conjunction with immunohistochemistry. In our experimental conditions OGD-insult followed by 24 h of recovery caused the damage of neuronal cells in CA1. At 1 week cell death appears all over the hippocampus. We found that expected stimulation of endogenous neurogenesis fails as a source of compensation for the lost neurons in OGD-treated cultures. The modulation of culture microenvironment after ischemia favors the dominant proliferation of glial cells expressed by the enhancement of newly-generated oligodendrocyte progenitors. In addition, during our study we also detected some BrdU labeled nuclei encapsulated by GFAP positive processes. However, the majority of BrdU positive cells expressed microglial specific stain, particularly pronounced in CAlarea. The OGD-promoted responses involved activation of metalloproteinases, which matches the progression of gliogenesis. On the other hand, the high activity of MMPs associated with microglial cells implicate their involvement in the mechanism participating in OGD-induced cell damage.
12
Content available remote

Modeling the neurovascular niche: Implications for recovery from (CNS) injury

67%
Madri J. A.
Journal of Physiology and Pharmacology. Supplement
|
2009
|
tom 60
|
nr 4
13

Recent advances in neonatal pain research

67%
Kostarczyk E.
Folia Morphologica. Supplement
|
1999
|
tom 58
|
nr 2
14

Genetic models to study adult neurogenesis

67%
Filipkowski R. K., Kiryk A., Kowalczyk A., Kaczmarek L.
Acta Biochimica Polonica
|
2005
|
tom 52
|
nr 2
In the central nervous system (CNS) generation of new neurons continues throughout adulthood, when it is limited to the olfactory bulb and hippocampus. The knowledge regarding the function of newly-generated neurons remains limited and is vigorously investigated using diverse approaches. Among these are genetically modified mice, most of them of knock-out type (KO). Results from 23 diverse KO mouse models demonstrate the importance of particular proteins (growth factors, nitric oxide synthases, receptors, cyclins/cyclin-associated proteins, transcription factors, etc.) in adult neurogenesis (ANGE) as well as separate it from developmental neurogenesis. These results bring us closer to revealing the function of newly generated neurons in adult brains.
15

Ependymal cells and astrocytes generate neurons

67%
Wozniak W.
Folia Morphologica. Supplement
|
1999
|
tom 58
|
nr 2
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.