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Amount of food supplied to nestlings by their parents is considered to affect the development of nestling physiological condition. In this study we supplied parental Great Tits Parus major with extra food, larvae of Tenebrio molitor, put into feeders close to nest-boxes, assuming that this should facilitate parental care and, as a consequence, nestling nutrition. The following nestling characteristics measured 13 days after hatching were analysed: body mass, haematocrit, blood concentrations of haemoglobin, glucose and triglycerides, heterophil-to-lymphocyte ratio (H/L), and patagium swelling after PHA injection. Nestlings from extra food broods were significantly heavier than control ones. They also had lower H/L, which indicated lower stress. No other variable was significantly affected by the experiment. Possibly, the rainy weather and non-restrictive natural trophic conditions during the experiment caused weakening of the net benefits from extra food.
Insectivorous birds have very diversified diet, but particular species usually show some specialisation, which leads to a varying level of dependence on special prey. Their reproductive cycles are dependent on the availability of appropriate arthropods; in the case of Blue Tits Cyanistes caeruleus and Great Tits Parus major reproduction is usually coordinated with the availability of caterpillars as the key food for nestlings. Therefore a picture of nestling diet, with some estimates of the actual frequency of caterpillars and alternative prey, is an important component of explanations of aspects of Tit life-histories. As in most cases a rough assessment of diet composition and relative proportions of prey items is satisfactory, we suggest that faecal analysis is a feasible method to get such a picture. Droppings may be collected to examine the diet of individual nestlings grouped in broods, at a particular age stage or at many stages reflecting development. The most time-consuming part of this method includes segregation and identification of prey remains in the laboratory. We draw attention to the procedures and the most diagnostically useful features of arthropod prey of Tits. Especially, we provide clues to identification of the remains of different arthropods. As an example, clypeus proved to be the most valuable structure to identify caterpillars, while chelicerae were the most diagnostically significant in Arachnids. Exemplary results on diet spectrum for the Blue Tit and Great Tit are also presented. Faecal analysis is fast and effortless at the sampling stage, with almost all effort being postponed to the stage of laboratory work.
The study was conducted in the Lake Karaś reserve (N Poland), where the density of Willow Warblers in osier and birch thickets reached 28.7-33.3 p/10 ha. Collar rings were used to collect the food brought to the nestlings. 487 food samples were collected from 26 nests, comprising 2573 food items. 167 of the samples were from females (652 individual food items), 95 from males (616 food items). The nestlings were fed mainly on Arthropoda (94.6%) and Mollusca (5.4%). The dominant food items were Diptera (29.8%), Homoptera (28.7%), Ephemeroptera (10.5%), Araneida (7.6%) and Trichoptera, (6.5%). The composition of the dominant food items and the average mass of food deliveries changed markedly with nestling growth. The types of food brought by each sex also varied significantly. Males brought heavier portions of food consisting of larger numbers of lighter prey. However, the composition of these portions was not as diverse as those brought by females. This may be related to differences between males and females in their respective territories and foraging techniques.
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