Cholecystokinin like activity was measured in duodenal perfusates obtained from a 30 cm closed loop of the proximal duodenum in anaesthetized neonatal calves. The concentration and the output of the CCK activity in the duodenal perfusates increased significantly during pectrical stimulation of the vagus nerve, whereas the blood plasma concentration of CCK (external jugular vein and portal vein) remained unchanged and lower than that found in the duodenum. On the other hand, intravenous infusion of CCK raised the blood plasma but not the duodenal CCK concentrations. In conclusion, CCK-like activity is present in the duodenal lumen. The luminal release mechanism is at least partly regulated by the vagus nerves.
3
Dostęp do pełnego tekstu na zewnętrznej witrynie WWW
During the early postnatal period in calves various adaptational changes occur. These functional, morphological and also metabolic alteration are reflected by blood plasma protein changes as they are secreted and shed from many cells and tissues. Blood plasma protein pattern of an adult cattle differs in some respect when compared with neonatal calves. There exist a very few data concerning 2-D maps of neonatal calves blood plasma. The above prompted us to establish protein pattern of this biological fluid characteristic of healthy, 7 day old, Polish Black-and-White (Polish Friesian) breed calves. Blood plasma proteins of the isoelectric point ranging from 4.0 to 7.0 were analyzed by the aid of high resolution two-dimensional electrophoresis (2-DE). Subsequently, 79 excised protein spots corresponding to 23 different gene products were identified using matrix-assisted laser desorption/ionisation mass spectrometer (MALDI-TOF MS). Protein map obtained in the present study may be useful in assessing the changes in the calves blood plasma protein profiles occurring in response to different physiological and/or pathophysiological factors.
4
Dostęp do pełnego tekstu na zewnętrznej witrynie WWW