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2-/4-/2-(4-chlorbenzamido) ethyl/phenoxy/-2-methyl-propionic acid (CBMP) reduced the intensity of cytopathic effect, delayed the time of its appearance for 24—48 hours and finally caused a significant lowering of ТСID₅₀ titer of herpes simplex in monkey kidney cell cultures and of pseudorabies virus in chick embryo cell cultures. On the other hand CBMP did not exert any effect on the multiplication of Roakin strain of NDV in chick embryo cell cultures. The preparation did not inactivate directly the viruses being examined and did not influence their adsorption. It is suggested that antiviral effect of CBMP is due to disordering the lipid metabolism in cells what causes the disturbances of virus envelope synthesis.
Foot-and-mouth disease virus (FMDV) is a single-stranded, positive-sense RNA virus belonging to the genus Aphthovirus in the family Picornaviridae. FMDV enters cells via the mechanism of receptor-mediated endocytosis in which the low pH of the endosomal compartment triggers uncoating of the viral genome. FMDV enters cells by attaching itself to cellular receptor molecules of the integrin family. For FMDV the receptor has been identified as the Arg-Gly-Asp (RGD) binding integrin. The integrin-binding RGD is located in the G-H loop of VP1 and it is highly conserved among all seven serotypes. The FMDV genome organization is similar to that of other picornaviruses. The genome is composed of three parts, the 5’ non-translated region (5’NTR), the coding region and the 3’ non-translated region (3’NTR) containing a heteropolymeric segment and poly(A) tail, which is required for viral replication. The 5’NTR plays important roles in cap-independent translation initiation of the viral polyprotein and in viral genome replication. After translation, the polyprotein is cleaved into four primary cleavage products: the amino terminal L protease; P1-2A, the precursor of the capsid proteins; 2BC and P3 which are cleaved into nonstructural proteins.
The infection of dogs with distemper virus (CDV), adenovirus type 1 (CAV-1) and parvovirus (CPV) is still being diagnosed by practitioners, even in vaccinated dogs. Laboratory techniques used for the isolation and identification of these viruses are usually time consuming and often their accuracy is not satisfactory. The presented studies concentrated on the adaptation of PLA (Peroxidase Linked Assay) for the evaluation of CDV, CAV-1 and CPV replication in tissue cultures. Comparable studies on titration of viruses tested on the basis of cytopathic effects and results of PLA revealed that the titre of CDV and CPV evaluated by CPE was lower than calculated using PLA. The difference was equal to 0.6 log for CDV and 0.7 log CPV. These results confirm four to five-fold higher sensitivity of PLA. Titre of adenovirus type 1 measured by both methods did not demonstrate significant differences which can be explained by production of regular and characteristic CPE and rapid spread of cell destruction. Results of PLA revealed that the expression of viral antigens takes place between 18 and 24 hours after cell inoculation, whereas the first cytopathic effect was visible after 48-96 hours. The repeatability of results obtained by PLA was significantly higher with p<0.05 than titration of viruses on the bases of CPE in the case of CAV-1 and CDV. The presented results confirm the high sensitivity and specificity of PLA, which could be widely used in studies on CDV, CAV-1 and CPV.
W badaniach prowadzonych uprzednio w Katedrze i Zakładzie Mikrobiologii Lekarskiej stwierdzono, że niektóre pochodne naturalnych seskwiterpenów oraz taksolu wykazywały aktywność przeciwwirusową, hamując namnażanie wirusa HSV-1 in vitro. Celem niniejszej pracy było uzyskanie wglądu w przeciwwirusową i przeciwbakteryjną aktywność siedmiu nowo zsyntety- zowanych związków chemicznych tej grupy.
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