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Krawczuk I. and Górski J.: The significance of plasma triacylglycerols in metabolism of free fatty acids and triacylgrylcerols in rat skeletal muscles. Acta Physiol. Pol., The continuous uptake of plasma triacyl- gylcerols has been shown to be necessary to maintain the stable resting concentration of free fatty acids and triacylglycerols only in a muscle composed of fast - twitch oxidative gylcolytic fibres.
The purpose of this work was to compare the fatty acid composition of the following reserve lipid tissues: mane, perirenal and intermuscular, and muscles: semimembranous with thigh adductor, middle gluteal, dorsal longissimus and infraspinous with serratus. Perirenal tissue contained significantly less unsaturated fatty acids (59.96%, p<0.05) compared to mane (61.43%, p<0.05) and intermuscular (61.24%, p<0.05) tissues. The tissues differed in the content of palmitic, stearic, oleopalmitic, miristic, and linolenic acids. All the tissues had a predominance (from 2.87% to 3.92%) of linolenic acid over linoleic acid, unique to animal fats. The percentage of particular fatty acids, except palmitic and linoleic acids in all muscle lipids was very similar. In contrast to reserve lipid tissues, the content of linoleic acid in muscle lipids was higher by 1.68% to 4.18% than of linolenic acid.
Muscle lipids of ten fish species (freshwater: zander, whitefish, roach, burbot and crucian carp; farmed: rainbow trout and carp; and Baltic species: garfish, flounder and sprat) were analysed for fatty acids composition (GC/MS), lipid fractions (HPLC) and susceptibility to oxidation. 100 g of meat of lean freshwater and Baltic fish (roach, flounder, burbot and zander) can provide 50% less n-3 PUFAs than the meat of rainbow trout or whitefish and 4-6 times less than sprat. The susceptibility to oxidation of freshwater fish lipids was higher than in the Baltic species of similar PUFAs content.
Lipid was extracted from the lipid containing muscle of Liner silver grunter (Pomadasys hasta) by solvent extraction and then purified by standard method. The physico-chemical properties of lipid sample were determined and compared with those of other standard fats and oils. Fatty acid composition of the lipid sample was investigated by Thin Layer Chromatographic (TLC) examination. Gas Liquid Chromatographic (GLC) examination was performed by the methyl esters mixture prepared from the lipid sample. The de-oiled muscle of the fish was studied for the determination of ash content, fiber content, moisture content and protein content. The lipid containing selected muscle was analyzed quantitatively for the determination of percentages of protein, cholesterol and minerals (N, P, K, Ca, and Na). The lipid containing muscle was also analyzed for the determination of the amount of eleven metals by using Atomic Absorption Spectrophotometric method. The lipid sample was evaluated by microbial activity (bacterial activity against nine bacteria and fungal activity against seven fungi).
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