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Introduction. 110-m and 400-m hurdles races are based on anaerobic energy metabolism which can induce muscle fatigue and muscle fiber damage. The most common biochemical parameters used in controlling athletes’ training loads and post-exercise fatigue are blood lactate (La) concentration and creatine kinase (CK) activity. Aim of Study. The aim of the study was to determine and compare runners’ biochemical response after 110-m and 400-m hurdles races. The influence of warm-up before both races was taken into consideration. Material and Methods. Eight male hurdlers took part in this research. They were subjected to two test exercises: a 110-m and a 400-m hurdles race. During each test, pre-warm-up, post-warm-up, and post-exercise capillary blood from a fingertip was collected to determine the La concentration and CK activity. Furthermore, during both sprint runs the athletes’ time (to the nearest 0.01 s) and heart rate (HR) were measured. Results. Each kind of exercise test increased the La concentration and CK activity. More significant changes of both biochemical parameters occurred after the 400-m race. Furthermore, after each warm-up significant increases of La and CK levels were observed. After the 400-m hurdles race higher HR values were noted (184.50 ± 8.32 compared to 177.50 ± 11.14 after the 110-m sprint). Conclusions. Both specialist warm-up and 110 and 400 meters hurdles races lead to significant changes in athletes’ physiological and biochemical blood parameters. La concentration and CK activity demonstrate greater muscle fatigue and muscle fiber damage after a 400-m than a 110-m hurdles race.
During human locomotion the ability to generate and sustain mechanical power output is dependent on the organised variability in contractile and metabolic properties of the muscle fibres that comprise the active muscles. In studies of human exercise we have used a micro-dissection technique to obtain fragments of single muscle fibres from needle biopsies before and after exercise. Each fibre fragment is divided into two parts. One part is used to characterize the fibre type in respect of the heavy chain myosin isoform expressed. The other part of the fragment is analysed for high energy phosphate concentrations. Fibres are classified on the basis of expressing either type I, type IIA, or type IIX myosin heavy chain isoforms. It should be noted however that in the type II population many fibres co-express both IIA and the IIX isoforms and we therefore characterize these fibres on the basis of the degree of co-expression. We have used this technique to examine the time course of high energy phosphate concentration and fatigue in different fibre populations during exercise. The progressive reduction of power during maximal sprint efforts may be interpreted as the cumulative effect of metabolic depletion in successive fibre type populations from IIX to IIXa to IIAx to IIA to I. One important application of the micro-dissection technique is that PCr content may also be used as a very sensitive metabolic marker for fibre type recruitment during very short duration concentric, isometric and eccentric exercise.
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